The secretome from embryonic stem cell cardiomyogenesis: Same signals, different cellular feedbacks

Abstract

Ischemic heart diseases are a global health problem that requires the search for alternative therapies to the current treatments. Thus, an understanding of how cardiomyogenic signals can affect cellular behavior would allow us to create strategies to improve the cell recovery in damaged tissues. In this study, we aimed to evaluate the effects of the conditioned medium (CM), collected at different time points during in vitro cardiomyogenesis of human embryonic stem cells (hESCs), to direct cell behavior. We assayed different cell types to demonstrate noncytotoxic effects from the collected CM and that the CM obtained at initial time points of cardiomyogenic differentiation could promote the cell proliferation. Otherwise, the secretome derived from cardiac committed cells during cardiomyogenesis was unable to improve angiogenesis or migration in endothelial cells, and ineffective to stimulate the differentiation of cardioblasts or increase the differentiation efficiency of hESC. Therefore, we demonstrated that the effectiveness of the CM response varies depending on the cell type and the differentiation step of hESC-derived cardiomyocytes.

Comparative analysis of the potential of the secretomes of cardiac resident stromal cells and fibroblasts

“…To assess the ability of CMs to stimulate the HUVEC or H9c2 migration, we performed a scratch-wound assay, in line with prior studies. 8 Briefly, 1 Â 10 4 cells/well (96-well plates) were maintained in culture until 100% confluence was reached. We then made a scratch in the monolayer using a 200 μl micropipette tip.…”