The secretome from bovine mammosphere-derived cells (MDC) promotes angiogenesis, epithelial cell migration, and contains factors associated with defense and immunity

Abstract

Treatment of bovine mastitis with intramammary antibiotics is common, yet several concerns exist including failed efficacy for individual hosts or pathogens and the inability of approved drugs to revert mastitis-induced tissue damage to healthy tissue capable of returning to full milk production. These issues, in addition to aspects of public health such as accidental antibiotic residues in saleable milk and the potential for antimicrobial resistance, support the need to find alternative therapies for this costly disease. This study shows that the secretome, or collective factors, produced by mammosphere-derived cells (MDC) promotes angiogenesis, epithelial cell migration, and contains proteins associated with immunity and defense; all of which are necessary for healing damaged mammary gland tissue. Furthermore, we found that the MDC secretome remains effective after freezing and thawing, enhancing its therapeutic potential. Our results provide a foundation for further characterization of the individual secreted factors and the rationale for using the MDC secretome as a complementary treatment for bovine mastitis.

Secreted sphingomyelins modulate low mammary cancer incidence observed in certain mammals

“…Canine mammary gland samples were collected from healthy, non-lactating, research beagles (6-10 years old), euthanized for reasons unrelated to this study, by excising at least 2 cm 2 of tissue near the nipple. Samples were processed to establish and maintain MDEC cultures, exactly as described before 11,38,39 . Mammary fibroblasts, isolated from the same mammary tissues to provide tissue-matched cells, were cultured in cell line medium, consisting of Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/ streptomycin (P/S) (cell line medium).…”

Comprehensive Identification of the Human Secretome as Potential Indicators in Treatment Outcome of HPV-Positive and -Negative Cervical Cancer Patients

“…All eukaryotic cells use essentially the same pathway for the synthesis and sorting of secreted proteins (referred as the secretome, short for SPRome). These proteins are important for maintaining cell-cell communication and controlling proliferation and play critical roles in metabolism [9] and immune systems [10]. Many of the secreted proteins can be released from the cells and enter into the blood circulation and therefore have similar expression patterns in tissues and blood samples.…”

Secrets of Mammary Stem Cells Secretome

“…In this study, in addition to enriched expression of ephrin receptors and integrins, the activity of Wnt signalling pathway was uniquely detected in the MaSC [2]. In a recent publication of Scientific Reports journal, researchers from Cornell University, Ithaca, NY has defined secretome of mammosphere derived MaSC and opened up new doors for the possible treatment of bovine mammary gland infections [3].…”