The Secretion of Immuno-Reactive Luteinizing Hormone and Follicle-Stimulating Hormone by the Human Foetal Pituitary in Organ Culture

Abstract

Foetal human pituitaries of 8\p=n-\18weeks gestational age were maintained in tissue culture for up to 6 days. No morphological change was observed in the tissue during this period. Radioactive glucosamine was continuously incorporated into medium protein and the levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH) and growth hormone in the medium increased with the duration of the culture. Theophylline stimulated the release of both LH and FSH consistently over the 6 days, and the total amount of each hormone released over this period was significantly greater than that in unstimulated control cultures. Cyclic adenosine 3' , 5' \ x=req-\ monophosphate stimulated FSH release to some extent but had no significant effect on LH release.

Differential Release of Individual Gonadotrophins by the Human Foetal Pituitary Gland After Stimulation by Cyclic Amp and Sodium Fluoride

“…Cyclic adenosine 3\m=' \,5\m=' \-monophosphate (cAMP), while enhancing IRFSH release, had only a minimal effect on the levels of IRLH secreted. The present study extends this work utilizing the organ culture technique and radioimmunoassay methods described previously (Groom et al 1971). …”

“…Work in this laboratory has demonstrated the ability of theophylline to stimulate the release of immunoreactive luteinizing hormone (IRLH) and follicle-stimulating hormone (IRFSH) in organ cultures of human foetal pituitary glands (Groom, Groom, Cooke & Boyns, 1971). Cyclic adenosine 3\m=' \,5\m=' \-monophosphate (cAMP), while enhancing IRFSH release, had only a minimal effect on the levels of IRLH secreted.…”

Immunoradiometric and Two-Site Assay of Human Follicle-Stimulating Hormone

“…Plasma was separated by centri¬ fugation and stored at -20°C before assay. Follicle-stimulating hormone was measured in 17 patients and the results were compared with those obtained pre¬ viously on the same samples by a double antibody radioimmunoassay technique in which the 2nd IRP human menopausal gonadotrophin was used as standard (Groom, Groom, Cooke & Boyns, 1971). There was good correlation between the two assays (r = 0-85, P < 0-001) and the mean ± s.e.m.…”

Characterisation of breast skin temperature rhythms of women in relation to menstrual status

“…Saliva was also stored at -20°C after collection. Plasma luteinizing hormone (LH) and follicle stimulating hormone (FSH) were assayed by double antibody radioimmunoassays shown to be specific for these hormones (Groom et al 1971). Concentrations of progesterone and 17a-hydroxyprogesterone in saliva were determined by procedures recently developed in the Institute (Walker et al 1978(Walker et al , 1979a.…”