The secondary laticifer differentiation in rubber tree is induced by trichostatin A, an inhibitor of histone acetylation

Zhang, Shixin; Wu, Shan; Weimin, Tian

doi:10.15302/j-fase-2016125

Cited by 6 publications

(7 citation statements)

References 27 publications

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“…Besides this, we found three genes (TAZ, GNAT, and SNF2) in the FDE dataset that are related to histone acetylation. The specific patterns of histone acetylation could alter the rate of chromatin remodelling in many ways 112 and in particular, a histone deacetylase inhibitor, Trichostatin A, was reported to induce induce SLD in H. brasiliensis 24 . This further suggests the importance of histone acetylation in the regulation of SLD.…”

Section: Resultsmentioning

confidence: 99%

“…Besides, wounding which is often done during commercial exploitation, is an important factor that induces laticifer ring formation 20,21 . In addition, the application of exogenous jasmonic acid (JA), methyl jasmonate, linolenic acid (LA), hydrogen peroxide, polyethylene glycol 6000, trichostatin A, or coronatine could induce the secondary laticifer differentiation (SLD) in H. brasiliensis but this is not the case for ethylene, salicylic acid or abscisic acid treatment 18,22–24 . Among the factors that could induce SLD, JA is a phytohormone where its signalling pathway has been studied extensively 25,26 .…”

Section: Introductionmentioning

confidence: 99%

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Identification and characterization of jasmonic acid- and linolenic acid-mediated transcriptional regulation of secondary laticifer differentiation in Hevea brasiliensis

Loh

Othman

Singham

2019

Sci Rep

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Hevea brasiliensis remains the primary crop commercially exploited to obtain latex, which is produced from the articulated secondary laticifer. Here, we described the transcriptional events related to jasmonic acid (JA)- and linolenic acid (LA)-induced secondary laticifer differentiation (SLD) in H. brasiliensis clone RRIM 600 based on RNA-seq approach. Histochemical approach proved that JA- and LA-treated samples resulted in SLD in H. brasiliensis when compared to ethephon and untreated control. RNA-seq data resulted in 86,614 unigenes, of which 2,664 genes were differentially expressed in JA and LA-induced secondary laticifer harvested from H. brasiliensis bark samples. Among these, 450 genes were unique to JA and LA as they were not differentially expressed in ethephon-treated samples compared with the untreated samples. Most transcription factors from the JA- and LA-specific dataset were classified under MYB, APETALA2/ethylene response factor (AP2/ERF), and basic-helix-loop-helix (bHLH) gene families that were involved in tissue developmental pathways, and we proposed that Bel5-GA2 oxidase 1-KNOTTED-like homeobox complex are likely involved in JA- and LA-induced SLD in H. brasiliensis. We also discovered alternative spliced transcripts, putative novel transcripts, and cis-natural antisense transcript pairs related to SLD event. This study has advanced understanding on the transcriptional regulatory network of SLD in H. brasiliensis.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification and characterization of jasmonic acid- and linolenic acid-mediated transcriptional regulation of secondary laticifer differentiation in Hevea brasiliensis

Loh

Othman

Singham

2019

Sci Rep

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“…At present, there are many reports about the effect of methyl jasmonate on development of laticifers, for example, inducing the biosynthesis of secondary plant metabolites and stimulating the formation and differentiation of milk ducts [11,12]. On this basis, comparative proteomics studies of latex in E. kansui laticifers treated with methyl jasmonate were conducted.…”

Section: Discussionmentioning

confidence: 99%

“…MeJA promotes the transcription and expression of the vegetative storage protein (VSP) in Medicago sativa [11], and also induces the biosynthesis of secondary plant metabolites. It has been reported that exogenous JAs can stimulate formation and differentiation of laticifers, a specific type of cell, in Hevea brasiliensis , and they can induce the production of numerous secondary metabolities, including latex [11,12].…”

Section: Introductionmentioning

confidence: 99%

iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of Euphorbia kansui L.

Fang

Yao

Zhang

et al. 2019

IJMS

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Autophagy is a well-defined catabolic mechanism whereby cytoplasmic materials are engulfed into a structure termed the autophagosome. Methyl jasmonate (MeJA), a plant hormone, mediates diverse developmental process and defense responses which induce a variety of metabolites. In plants, little is known about autophagy-mediated responses against MeJA. In this study, we used high-throughput comparative proteomics to identify proteins of latex in the laticifers. The isobaric tags for relative and absolute quantification (iTRAQ) MS/MS proteomics were performed, and 298 proteins among MeJA treated groups and the control group of Euphorbia kansui were identified. It is interesting to note that 29 significant differentially expressed proteins were identified and their associations with autophagy and ROS pathway were verified for several selected proteins as follows: α-L-fucosidase, β-galactosidase, cysteine proteinase, and Cu/Zn superoxide dismutase. Quantitative real-time PCR analysis of the selected genes confirmed the fact that MeJA might enhance the expression of some genes related to autophagy. The western blotting and immunofluorescence results of ATG8 and ATG18a which are two important proteins for the formation of autophagosomes also demonstrated that MeJA could promote autophagy at the protein level. Using the electron microscope, we observed an increase in autophagosomes after MeJA treatment. These results indicated that MeJA might promote autophagy in E. kansui laticifers; and it was speculated that MeJA mediated autophagy through two possible ways: the increase of ROS induces ATG8 accumulation and then aotophagosome formation, and MeJA promotes ATG18 accumulation and then autophagosome formation. Taken together, our results provide several novel insights for understanding the mechanism between autophagy and MeJA treatment. However, the specific mechanism remains to be further studied in the future.

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“…The number of the secondary laticifers in the trunk bark is closely related to the rubber yield. The secondary laticifer is differentiated from vascular cambia (Hao and Wu, 2000 [6]), and induced by such factors as jasmonic acid (JA) [6][7][8], mechanical wounding [7], and coronatine (COR) [9][10][11], an active jasmonate homolog [12] Available data show that both the secondary laticifer differentiation [6,13] and the rubber biosynthesis [14] are positively controlled by JA signalling. Therefore, the MYC members in rubber trees should be involved in regulating Forests 2022, 13, 531 2 of 13 such processes.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of MYC Transcription Factor Family Genes in Rubber Tree (Hevea brasiliensis Muell. Arg.)

Zhang

Chao

et al. 2022

Forests

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Myelocytomatosis (MYC) transcription factors play a core regulator in the jasmonic acid signaling pathway, which regulates the secondary laticifer differentiation and rubber biosynthesis in rubber tree (Hevea brasiliensis). However, there are currently no reports on the MYC gene family in rubber trees, an important industrial raw material crop worldwide. In the present study, 32 HblMYCs were isolated and identified. The diversity in gene structure and presence of various cis-regulatory elements in promotors suggest that HblMYCs participate in various biological processes. Based on the expression patterns in the cambium region and laticifer in, respectively, response to coronatine (COR) and tapping, and the phylogenetic relationship with the MYCs that have been functionally identified in other plants, the HblMYC24 and HblMYC30 may be related to laticifer differentiation while the HblMYC6, HblMYC11 and HblMYC15, as well as HblMYC16 and HblMYC21, may positively regulate rubber biosynthesis. The results provide a foundation for understanding the molecular mechanism of jasmonate signaling in regulating laticifer differentiation and rubber biosynthesis in rubber tree.

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The secondary laticifer differentiation in rubber tree is induced by trichostatin A, an inhibitor of histone acetylation

Cited by 6 publications

References 27 publications

Identification and characterization of jasmonic acid- and linolenic acid-mediated transcriptional regulation of secondary laticifer differentiation in Hevea brasiliensis

Identification and characterization of jasmonic acid- and linolenic acid-mediated transcriptional regulation of secondary laticifer differentiation in Hevea brasiliensis

iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of Euphorbia kansui L.

Genome-Wide Identification and Expression Analysis of MYC Transcription Factor Family Genes in Rubber Tree (Hevea brasiliensis Muell. Arg.)

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