The second intracellular loop of the yeast Trk1 potassium transporter is involved in regulation of activity, and interaction with 14–3-3 proteins

Masaryk, Jakub; Kale, Deepika; Pohl, Pavel; Ruiz-Castilla, Francisco J.; Zimmermannová, Olga; Obšilová, Veronika; Ramos, José; Sychrová, Hana

doi:10.1016/j.csbj.2023.04.019

Cited by 3 publications

(3 citation statements)

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“…However, unlike the aforementioned negative regulation of Nha1, Bmh binding activates Trk1. At low K + levels, Trk1 activity depends on two residues of the second intracellular loop (Ser 882 and Thr 900 ), but Thr 900 phosphorylation alone is responsible for Bmh binding, in line with the finding that the Thr 900 -to-Ala mutation markedly slows growth under low K + (Masaryk et al, 2023). Yet again, though, the precise molecular mechanisms whereby Bmh proteins modulate the function of Nha1 and Trk1 channels remain unknown.…”

Section: -3-3 Proteins Regulate Yeast Ion Transporters Trk1 and Nha1supporting

confidence: 72%

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The yeast 14-3-3 proteins Bmh1 and Bmh2 regulate key signaling pathways

Obsilova,

Obsil

2024

Front. Mol. Biosci.

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Cell signaling regulates several physiological processes by receiving, processing, and transmitting signals between the extracellular and intracellular environments. In signal transduction, phosphorylation is a crucial effector as the most common posttranslational modification. Selectively recognizing specific phosphorylated motifs of target proteins and modulating their functions through binding interactions, the yeast 14-3-3 proteins Bmh1 and Bmh2 are involved in catabolite repression, carbon metabolism, endocytosis, and mitochondrial retrograde signaling, among other key cellular processes. These conserved scaffolding molecules also mediate crosstalk between ubiquitination and phosphorylation, the spatiotemporal control of meiosis, and the activity of ion transporters Trk1 and Nha1. In humans, deregulation of analogous processes triggers the development of serious diseases, such as diabetes, cancer, viral infections, microbial conditions and neuronal and age-related diseases. Accordingly, the aim of this review article is to provide a brief overview of the latest findings on the functions of yeast 14-3-3 proteins, focusing on their role in modulating the aforementioned processes.

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Section: -3-3 Proteins Regulate Yeast Ion Transporters Trk1 and Nha1supporting

confidence: 72%

“…(E) Different mechanisms of regulation of yeast transporters by 14-3-3 proteins. 14-3-3 binding downregulates the cation efflux activity of Nha1 but upregulates the activity of the potassium transporter Trk1 ( Smidova et al, 2019 ; Masaryk et al, 2023 ). (F) Endocytosis regulation.…”

Section: 14-3-3 Proteins Regulate Fundamental Cellular Processes In Y...mentioning

confidence: 99%

The yeast 14-3-3 proteins Bmh1 and Bmh2 regulate key signaling pathways

Obsilova,

Obsil

2024

Front. Mol. Biosci.

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“…In particular, calcium contributes to cell wall stability, whereas copper and iron function as enzyme cofactors, driving diverse reactions. Potassium maintains cellular equilibrium and zinc is critical in enzyme regulation and yeast growth (Masaryk et al , 2023). However, the presence of mercury alongside these mineral nutrients is noteworthy due to its toxic nature.…”

Section: Discussionmentioning

confidence: 99%

Local staples, and global solutions: optimizing the effect of traditional fermentation on heavy metals and mineral nutrients

Anyimah-Ackah

2023

NFS

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Purpose This study aims to examine the effect of traditional fermentation on gari’s total heavy metal and mineral nutrient content. Design/methodology/approach This study used a quantitative approach, descriptive-analytical design to baseline the risk of heavy metals and experimental design to assess the effect of traditional fermentation. Data were analyzed using descriptives, univariate and multivariate analysis. Findings Although gari is rich in mineral nutrients (total calcium 3.9 ± 0.1 g/kg, copper 5.5 ± 0.02 mg/kg, iron 97.1 ± 5.8 mg/kg, potassium 9.1 ± 0.29 g/kg and zinc 3.4 ± 0.11 mg/kg), the significant levels of heavy metals (total arsenic 1.2 ± 0.01, cadmium 2.5 ± 0.04, lead 1.7 ± 0.01, mercury 2.8 ± 0.01 and tin 1.7 ± 0.02 mg/kg) present are a cause for concern. The results further suggested that traditional fermentation has reductive effects on some heavy metals and stabilizing or concentrating effects on mineral nutrients. Research limitations/implications This paper provides evidence that traditional fermentation may have exploitable differential effects on heavy metal contaminants and mineral nutrients that should be further explored. Practical implications Thise study reports fermentation implications for mitigating food with high heavy metal contaminants with minimal nutrient loss. Originality/value This study fulfills an identified need to optimize traditional fermentation to ensure food safety and nutrient security.

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