The search for sulfate-reducing bacteria in mat samples from the lost city hydrothermal field by molecular cloning

Microbial utilization of abiogenic carbon and hydrogen in a serpentinite-hosted system

“…The S-isotope compositions of dissolved sulfate are consistent with sulfate reduction, with d 34 S values ranging from +21& (seawater compositions) to +30&, versus Vienna Canyon Diablo Troilite (Kelley et al, 2005). Lipids specific for sulfate reducing bacteria (SRB) have been identified in the chimneys (Kelley et al, 2005), as have genes closely related to the SRB Desulfotomaculum, which has been identified as the most likely sulfate reducer in the chimneys (Brazelton et al, 2006;Gerasimchuk et al, 2010).…”

“…Phylogenetic studies have identified sulfate reducing bacterial communities in the Lost City chimneys that are closely related to Desulfotomaculum alkaliphilum and Desulfotomaculum halophilum (Kelley et al, 2005;Brazelton et al, 2006;Gerasimchuk et al, 2010). These species have optimum growth temperatures of 53°C and 35°C, and maximum growth temperatures of 58°C and 40°C, respectively, and other Desulfotomaculum species grow at similar temperature ranges (Campbell and Postgate, 1965;Widdel and Pfennig, 1977;Rabus et al, 2006).…”

“…SO 4 2À has a slope of À1, while those of SO 4 2À vs. H 2 and HS À vs. H 2 are 0.14 and À0.22, respectively (Table 3, Fig. 7); lipids and genes specific to sulfate reducers have been found in the chimneys (Kelley et al, 2005;Brazelton et al, 2006;Gerasimchuk et al, 2010); thermodynamic calculations demonstrate that sulfate reduction can supply 3-27 times more metabolic energy in unmixed anaerobic fluids than methanogenesis (Table 5). 2.…”

Microbial diversity and adaptation to high hydrostatic pressure in deep-sea hydrothermal vents prokaryotes

“…The Bacteria domain is dominated by Epsilonproteobacteria detected in hydrothermal fluids, in sea water, hydrothermal sediments, microbial mats and in association with macrofauna (Reysenbach et al 2000(Reysenbach et al , 2002Alain et al 2002b;Teske et al 2002;Huber et al 2003;Page et al 2004;Suzuki et al 2005;Campbell et al 2006;Gerasimchuk et al 2010;Crépeau et al 2011;Sylvan et al 2012); other bacterial groups were also detected, such as Alpha-, Beta-, Delta and Gammaproteobacteria, Aquificales, Desulfobacteriales, Thermotogales, Deinococcus-Thermus, Deferribacteres, Firmicutes, CFB (Cytophaga-Flavobacteria-Bacteroidetes), Acidobacteria, Verrumicrobia and Planctomycetes (Alain et al 2002a;Reysenbach et al 2002;Teske et al 2002;Huber et al 2003;Page et al 2004;Cré-peau et al 2011;Orcutt et al 2011;Sylvan et al 2012). More than one hundred species of Bacteria (17 phyla, 72 genera, 113 species) and Archaea (3 phyla, 17 genera and 62 species) were isolated and cultured from deep-sea hydrothermal vents, mainly from the Pacific ocean (69-77 % of species) and, in lesser numbers, from the Atlantic (19-28 % of species) and Indian (2-4 % of species) Oceans.…”

Firmicutesis an Important Component of Microbial Communities in Water-Injected and Pristine Oil Reservoirs, Western Siberia, Russia

“…DsrB gene-targeted denaturating gradient gel electrophoresis (DGGE) has been developed for the rapid detection of sulphate reducing microorganisms in environmental samples (Geets et al 2006). DsrB gene-targeted PCR-DGGE has been successfully applied to the characterization of SRB communities in various environments (Geets et al 2006;Dar et al 2007;Foti et al 2007;Miletto et al 2007;Auvinen et al 2009;Gerasimchuk et al 2010). To our knowledge, there are few reports describing the use of this functional marker in oil fields (Agrawal and Lal, 2009;Guan et al 2014).…”