The Sea Cucumber Body Wall Extract Promoted in Vitro Maturation of NMRI Mice Follicles at Germinal Vesicle Stage

Abstract

Background: IVM (in vitro maturation) appear to be certified high pregnancy outcome. Therefore, attempt to find an appropriate culture system using natural products for increasing developmental competencies fascinating. Objectives: This experiment is aimed to evaluate the effect of sea cucumber methanol extract on in vitro maturation of immature mouse oocyte. Methods: In this experimental study, Preantral follicles at germinal vesicle stage were collected from 24-26 days NMRI female mouse ovaries and transferred to IVM medium supplemented with different concentration of sea cucumber methanol extract (5, 10, 20, 30 µg/mL). Antioxidant capacity of sea cucumber extract was evaluated using DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay. Further, oocyte maturation and TNF-α expression were recorded till day 10 th. Data were analyzed by one-way analysis of variance (ANOVA) through SPSS 16. Results: The percentage of arrested oocyte at germinal vesicle stage in the control group was significantly (P < 0.05) higher than treated group (20 µg/mL). There were significant (P < 0.01) differences in percentage of maturated oocyte to metaphase II (MII) stage between treated and control group. DPPH radical scavenging capacity and reduction of TNF-α expression in treated oocyte demonstrated that sea cucumber increased rate of oocyte maturation. In addition, the treated oocyte (20 µg/mL) achieved the highest percentage of MII stage. Conclusions: It was concluded that the sea cucumber extract with optimum concentration can improve oocyte maturation. Sea cucumber extract treatment can be suggested as a novel therapeutic strategy to be used in infertility modality in the future.

Sea cucumbers of the Arabian Peninsula and Iran – A review of historical and current research trends

“…Sea cucumbers from Iran have been the subject of various reproductive endocrinology research activities ( Golestani et al, 2016 , Moghadam et al, 2016 ). Methanol extracts of H. leucospilota promote oocyte maturation a in rats and further studies could lead to development of novel therapeutics to treat infertility ( Khalizadeh et al, 2016 ). Furthermore, peptides hydrolyzed from the muscles of AG sea cucumbers possess anticoagulant properties similar to Heparin ( Samira and Saber, 2017 ).…”

Bioactives From Marine Animals: Potential Benefits for Human Reproductive Health

“…The results show that the extraction can enhance the maturation level of oocytes. Furthermore, the treated oocytes at 20 µg/mL of this sea cucumber extract have the highest percentage of Metaphase II stage (Khalilzadeh et al, 2016). According to Riani et al (2017), sea cucumber also exhibits the pharmaceutical potential for enhancing the quality of male reproduction.…”

WITHDRAWN: Sea Cucumbers of the Arabian Peninsula and Iran - a review of historical and current research trends

“…Sea cucumbers from Iran have been the subject of various reproductive endocrinology research activities ( Golestani et al, 2016 , Moghadam et al, 2016 ). Methanol extracts of H. leucospilota promote oocyte maturation a in rats and further studies could lead to development of novel therapeutics to treat infertility ( Khalizadeh et al, 2016 ). Furthermore, peptides hydrolyzed from the muscles of APG sea cucumbers possess anticoagulant properties similar to Heparin ( Samira and Saber, 2017 ).…”