The sea bass Dicentrarchus labrax as a marine model species in immunology: Insights from basic and applied research

Miccoli, Andrea; Picchietti, Simona; Scapigliati, Giuseppe

doi:10.1016/j.aaf.2021.09.003

Cited by 4 publications

(3 citation statements)

References 103 publications

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“…To further evaluate the potential of engineered B. subtilis strains as oral vaccines for fish, both GFP and OmpK containing spores were mixed with commercial feed for European seabass and used to orally vaccinate juveniles for one month. European seabass is both an economically important species in aquaculture and a marine model species in immunology ( 101 ). By the end of the experiment, anti-GFP antibodies were detected in the serum of fish fed the GFP-carrying spores supplemented diet (and not in fish fed the placebo diet) using a purified GFP and a GFP-specific antibody.…”

Section: Discussionmentioning

confidence: 99%

Oral vaccination of fish against vibriosis using spore-display technology

et al. 2022

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Oral vaccines are highly demanded by the aquaculture sector, to allow mass delivery of antigens without using the expensive and labor-intensive injectable vaccines. These later require individual handling of fish, provoking stress-related mortalities.One possible strategy to create injection-free vaccine delivery vehicles is the use of bacterial spores, extremely resistant structures with wide biotechnological applications, including as probiotics, display systems, or adjuvants. Bacterial spores, in particular those of Bacillus subtilis, have been shown to behave as mucosal vaccine adjuvants in mice models. However, such technology has not been extensively explored against fish bacterial disease.In this study, we used a laboratory strain of B. subtilis, for which a variety of genetic manipulation tools are available, to display at its spores surface either a Vibrio antigenic protein, OmpK, or the green fluorescence protein, GFP. When previously vaccinated by immersion with the OmpK- carrying spores, zebrafish survival upon a bacterial challenge with V. anguillarum and V. parahaemolyticus, increased up to 50 - 90% depending on the pathogen targeted. Further, we were able to detect anti-GFP-antibodies in the serum of European seabass juveniles fed diets containing the GFP-carrying spores and anti-V. anguillarum antibodies in the serum of European seabass juveniles fed the OmpK-carrying spores containing diet. More important, seabass survival was increased from 60 to 86% when previously orally vaccinated with in-feed OmpK- carrying spores. Our results indicate that B. subtilis spores can effectively be used as antigen-carriers for oral vaccine delivery in fish.

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Section: Discussionmentioning

confidence: 99%

Oral vaccination of fish against vibriosis using spore-display technology

et al. 2022

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“…We believe that our results, obtained in an attempt to bridge the fields of biological oceanography and experimental toxicology, are biologically significant and actual because i) microlitter particles and fish specimens originated from the same sampling site, ii) microlitter cytotoxicity was measured by the well-established, highly-sensitive and unambiguous direct luciferase-based quantification of cellular ATP (Cree and Andreotti, 1997;Mahto et al, 2010) iii) primary cultures were obtained from organs that are key in ensuring immune barrier and competency and iv) the suitability of the strategy for testing for MP toxicity was overall demonstrated and recently reviewed in details (Revel et al, 2021). In addition, fish have been increasingly established as experimental models in the fields of biomedical sciences and toxicology because they share many similarities with higher vertebrates immunology-wise (Miccoli et al, 2021a;Scapigliati et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

First evidence of in vitro cytotoxic effects of marine microlitter on Merluccius merluccius and Mullus barbatus, two Mediterranean commercial fish species

Miccoli

Mancini²,

Saraceni

et al. 2022

Science of The Total Environment

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“…In addition, fish have been increasingly established as experimental models in the fields of biomedical sciences and toxicology because they share many similarities with higher vertebrates immunology-wise (Miccoli et al, 2021;Scapigliati et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

First evidence of in vitro cytotoxic effects of marine microlitter on Merluccius merluccius and Mullus barbatus, two Mediterranean commercial fish species

Miccoli

Mancini²,

Saraceni

et al. 2021

Preprint

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Marine litter, which is composed mainly of plastics, is recognized as one of the most serious threats to marine ecosystems and a global environmental concern. Microplastics (MPs) densities were estimated in all environmental compartments: marine organisms are highly exposed to and ingest them, resulting in disruption of biological functions. Ecotoxicological approaches have also started elucidating the potential severity of MPs in controlled laboratory studies, but the commercially-available and pristine materials employed hardly reflect the actual composition of the environmental litter, which can be contaminated by chemical pollutants or biological agents. Building on the lack of research employing marine environmental MPs or microlitter as a whole, we characterized the quantity and quality of litter in the coastal epipelagic and in the digestive tract of two commercially-relevant fish species, and exposed primary cell cultures of mucosal and lymphoid organs to marine microlitter. A concentration of 0.30 ± 0.02 microlitter items m-3 was found in the water column of the Northern Tyrrhenian sea. μFT-IR analysis revealed that particles of plastic origin, namely polypropylene, HDPE and polyamide, were present in 100% and 83.3% of M. merluccius and M. barbatus stomachs, respectively, which overall ingested 14.67 ± 4.10 and 5.50 ± 1.97 items. Microlitter was confirmed as a vector of bacteria, fungi and flagellates. Lastly, and for the first time, the apical end-point of viability was significantly reduced in splenic cells exposed in vitro to two microlitter conditions. Considering the role of the spleen in the mounting of adaptive immune responses, our results warrant more in-depth investigations for clarifying the actual susceptibility of the biota to anthropogenic microliter.Highlights0.30 ± 0.02 microlitter items m-3 was found in the coastal epipelagic Northern Tyrrhenian sea14.67 ± 4.10 and 5.50 ± 1.97 items were retrieved from hake and mullet stomach contentsA subsample of the ingested microlitter was of plastic originMicrolitter was validated as a carrier of bacteria, fungi and flagellatesSplenic cells exposed to both microlitter conditions for 72 hours suffered cytotoxicity

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The sea bass Dicentrarchus labrax as a marine model species in immunology: Insights from basic and applied research

Cited by 4 publications

References 103 publications

Oral vaccination of fish against vibriosis using spore-display technology

Oral vaccination of fish against vibriosis using spore-display technology

First evidence of in vitro cytotoxic effects of marine microlitter on Merluccius merluccius and Mullus barbatus, two Mediterranean commercial fish species

First evidence of in vitro cytotoxic effects of marine microlitter on Merluccius merluccius and Mullus barbatus, two Mediterranean commercial fish species

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