The Screening of Therapeutic Peptides for Anti-Inflammation through Phage Display Technology

Zhang, Kangran; Tang, Yezhong; Chen, Qin; Liu, Yang

doi:10.3390/ijms23158554

Cited by 11 publications

(9 citation statements)

References 118 publications

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“…[ 16 , 20 , 21 ] In this study, the length of the generated peptide was 5-mer, but the IC 50 of these functional peptides ranged from 0.53 to 4.35μM, which is comparable stronger than the isolated peptides. [ 16 ] Several studies highlighted the significance of using computer-aided ways for identification chemical compounds or designing peptide inhibitors of protein or enzyme, [ 38 ] suggesting computer-aided methods were gradually widely adopted. Compared with extraction from plants or mammals, the computer-aided method for designing peptides transferred abundant of laboratory work to computational work which avoids of time consumption and was labor saving.…”

Section: Resultsmentioning

confidence: 99%

Discovering peptide inhibitors of thrombin as a strategy for anticoagulation

Zhen,

Wang,

et al. 2024

Medicine

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Unusual blood clots can cause serious health problems, such as lung embolism, stroke, and heart attack. Inhibiting thrombin activity was adopted as an effective strategy for preventing blood clots. In this study, we explored computational-based method for designing peptide inhibitors of human thrombin therapeutic peptides to prevent platelet aggregation. The random peptides and their 3-dimentional structures were generated to build a virtual peptide library. The generated peptides were docked into the binding pocket of human thrombin. The designed strong binding peptides were aligned with the native binder by comparative study, and we showed the top 5 peptide binders display strong binding affinity against human thrombin. The 5 peptides were synthesized and validated their inhibitory activity. Our result showed the 5-mer peptide AEGYA, EVVNQ, and FASRW with inhibitory activity against thrombin, range from 0.53 to 4.35 μM. In vitro anti-platelet aggregation assay was carried out, suggesting the 3 peptides can inhibit the platelet aggregation induced by thrombin. This study showed computer-aided peptide inhibitor design can be a robust method for finding potential binders for thrombin, which provided solutions for anticoagulation.

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Section: Resultsmentioning

confidence: 99%

Discovering peptide inhibitors of thrombin as a strategy for anticoagulation

Zhen,

Wang,

et al. 2024

Medicine

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“…Because of their diverse biological functions, high affinity with specific targets, high membrane permeability, and many other special properties, peptides are considered important screening targets by the pharmaceutical industry [ 119 , 120 ]. The protein content in mushrooms is relatively high, with an average of approximately 23.80 ± 9.82 g of protein per 100 g of mushroom dry weight (d.w.) [ 121 ].…”

Section: Diversity Of Mushroom-derived Metabolites Beneficial To Ad A...mentioning

confidence: 99%

Multiple Metabolites Derived from Mushrooms and Their Beneficial Effect on Alzheimer’s Diseases

et al. 2023

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Mushrooms with edible and medicinal potential have received widespread attention because of their diverse biological functions, nutritional value, and delicious taste, which are closely related to their rich active components. To date, many bioactive substances have been identified and purified from mushrooms, including proteins, carbohydrates, phenols, and vitamins. More importantly, molecules derived from mushrooms show great potential to alleviate the pathological manifestations of Alzheimer’s disease (AD), which seriously affects the health of elderly people. Compared with current therapeutic strategies aimed at symptomatic improvement, it is particularly important to identify natural products from resource-rich mushrooms that can modify the progression of AD. This review summarizes recent investigations of multiple constituents (carbohydrates, peptides, phenols, etc.) isolated from mushrooms to combat AD. In addition, the underlying molecular mechanisms of mushroom metabolites against AD are discussed. The various mechanisms involved in the antiAD activities of mushroom metabolites include antioxidant and anti-neuroinflammatory effects, apoptosis inhibition, and stimulation of neurite outgrowth, etc. This information will facilitate the application of mushroom-derived products in the treatment of AD. However, isolation of new metabolites from multiple types of mushrooms and further in vivo exploration of the molecular mechanisms underlying their antiAD effect are still required.

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“…The functional properties of a protein can be modified through physical hydrolysis, one of which is sonication treatment (K. Zhang et al, 2022). Sonication treatment affected the functional and physicochemical properties of protein isolates due to disruption of the conformation of the protein structure (Zhu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Optimizatoin of Anti-Nuntritional Removal, Protein Extraction and Modification of Functional Properties of Cowpea (Vigna unguiculata L.) Protein with Sonication

Wijatniko,

Murdiati,

Eliza

et al. 2023

IFNP

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Cowpea has high protein content which makes it is potential to be utilized as a protein isolate. However, cowpeas contain some anti-nutritional compounds which can lower the nutritional quality of the cowpeas. It is necessary to reduce the antinutritional contents from the cowpeas.The objective of this study was to investigate the optimum treatments for antinutritional removal and protein extraction to produce protein isolate. Removal of antinutritional was performed by a combination of soaking treatment with the duration of 24, 48, and 72 hours with 5% NaHCO3 solution and boiling for 0, 2.5, and 5 minutes. The protein extraction was conducted by adjusting the pH to various pH at 2, 4, 6, 8, 10, 11, and 12.Soaking of cowpeas seeds for 48 hours with 5% NaHCO3 significantly reduced HCN levels to 0.72 ppm, phytic acid of 9.62 mg / g, and trypsin inhibitor of 7.02 mg / g. The protein concentrate was obtained from protein extraction by using optimum pH at 12 while precipitation of protein by using pH 4 with protein concentration at 70.9%. Sonication with 80%-30 minutes indicated an increase in the water holding capacity, oil holding capacity, emulsion stability, foaming capacity, and stability rather than untreated

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The Screening of Therapeutic Peptides for Anti-Inflammation through Phage Display Technology

Cited by 11 publications

References 118 publications

Discovering peptide inhibitors of thrombin as a strategy for anticoagulation

Discovering peptide inhibitors of thrombin as a strategy for anticoagulation

Multiple Metabolites Derived from Mushrooms and Their Beneficial Effect on Alzheimer’s Diseases

Optimizatoin of Anti-Nuntritional Removal, Protein Extraction and Modification of Functional Properties of Cowpea (Vigna unguiculata L.) Protein with Sonication

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