The Screening and Identification of a Batch of Oleaginous Yeasts

Abstract

Twenty three strains of oleaginous fungus were screened out from the excess sludge by Sudan black B staining method. After 26s rDNA sequence analysis and comparison, 10 of the strains were found to be Pichia jadini, 9 to be Trichosporon laibachii, and the other 4 to be Galactomyces geotrichum, which belong to molds. We focused on 7 strains of Tri. laibachii, and measured their biomass and oil yield. As a result, Tri. laibachii IEM-17 had the highest biomass, as well as the highest oil yield which reached 21.9%.