The PP 2A‐interactor TIP 41 modulates ABA responses in Arabidopsis thaliana

Plant Signaling & Behavior

Grillo

et al. 2018

Self Cite

Environmental conditions inform the rate of plant growth and development. The target of rapamycin (TOR) signalling pathway is a central regulator of plant growth in response to nutrients and energy, while abscisic acid (ABA) is a main mediator of abiotic stress responses. We recently characterized Arabidopsis TIP41, a predicted TOR pathway component involved in the ABA-mediated response to abiotic stress. Here, we report the ABA sensitivity of tip41 mutants, supporting the relation between TIP41 and the hormone pathway. The analysis of predicted TIP41 functional network identified several protein phosphatases. In particular, candidate protein interactors included catalytic subunits of type 2A protein phosphatases and protein phosphatases 6, which regulate different developmental processes and responses to environmental stimuli. These results provide important information on the role of TIP41 in the cross talk between TOR and ABA pathways.

mentioning

confidence: 99%

TIP41 network analysis and mutant phenotypes predict interactions between the TOR and ABA pathways

Plant Signaling & Behavior

Grillo

et al. 2018

Self Cite

“…Using RNA transcriptome data from potato cells adapted to gradually increasing concentrations of polyethylene glycol (PEG) we identified Arabidopsis orthologous genes, and functionally analysed their role in responses to ABA and osmotic stress (Ambrosone et al, 2015, 2017; Punzo et al, 2018). Following the same rationale, we focused on a DNA-damage-repair/toleration protein coding gene (EST617924, GenBank accession no.…”

Section: Resultsmentioning

confidence: 99%

DRT111/SFPS splicing factor controls ABA sensitivity in Arabidopsis seed development and germination

Possenti³

et al. 2020

Preprint

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RNA splicing is a fundamental mechanism contributing to the definition of the cellular protein population in any given environmental condition. DRT111/SFPS is a splicing factor previously shown to interact with phytochromeB and characterized for its role in splicing of pre-mRNAs involved in photomorphogenesis. Here, we show that DRT111 interacts with Arabidopsis Splicing Factor 1 (SF1), involved in 3’ splicing site recognition. Double and triple mutant analysis shows that DRT111 controls splicing of ABI3 and acts upstream of the splicing factor SUPPRESSOR OF ABI3-5 (SUA). DRT111 is highly expressed in seeds and stomata of Arabidopsis and is induced by long-term treatments with polyethylene glycol and ABA. DRT111 knock-out mutants are defective in ABA-induced stomatal closure and are hypersensitive to ABA during seed germination. Conversely, DRT111 over-expressing plants show ABA hyposensitive seed germination. RNAseq experiments show that in dry seeds, DRT111 controls expression and splicing of genes involved in response to osmotic stress and ABA, light signaling and mRNA splicing, including targets of ABSCISIC ACID INSENSITIVE3 (ABI3) and PHYTOCHROME INTERACTING FACTORs (PIFs). Consistently, expression of the germination inhibitor SOMNUS, induced by ABI3 and PIF1 is up-regulated in imbibed seeds of drt111-2 mutants. Altogether, these results indicate that DRT111 controls sensitivity to abscisic acid (ABA) during seed development, germination and stomatal movements and constitutes a point of integration of the ABA- and light-regulated pathways to control seed germination.One Sentence SummaryArabidopsis splicing factor DRT111/SFPS is required for ABA-mediated responses in seeds

“…Using RNA transcriptome data from potato (Solanum tuberosum) cells adapted to gradually increasing concentrations of polyethylene glycol (PEG) we identified Arabidopsis orthologous genes, and functionally analysed their role in responses to ABA and osmotic stress (Ambrosone et al, 2015(Ambrosone et al, , 2017Punzo et al, 2018). Following the same rationale, we focused on a DNA-damagerepair/toleration protein coding gene (EST617924, GenBank accession no.…”

Section: Drt111 Expression Is High In Seeds and Guard Cells And Is Inmentioning

confidence: 99%

DRT111/SFPS Splicing Factor Controls Abscisic Acid Sensitivity during Seed Development and Germination

Possenti³

et al. 2020

Plant Physiol.

Self Cite

RNA splicing is a fundamental mechanism contributing to the definition of the cellular protein population in any given environmental condition. DNA-DAMAGE REPAIR/TOLERATION PROTEIN 111/ SPLICING FACTOR FOR PHYTOCHROME SIGNALING (DRT111/SFPS) is a splicing factor previously shown to interact with phytochrome B and characterized for its role in splicing of pre-mRNAs involved in photomorphogenesis. Here, we show that DRT111 interacts with Arabidopsis thaliana Splicing Factor 1 (SF1), involved in 3′ splicing site recognition. Double and triple mutant analysis shows that DRT111 controls splicing of ABI3 and acts upstream of the splicing factor SUPPRESSOR OF ABI3-5 (SUA). DRT111 is highly expressed in seeds and stomata of Arabidopsis and is induced by long-term treatments of polyethylene glycol and abscisic acid (ABA). DRT111 knockout mutants are defective in ABA-induced stomatal closure and are hypersensitive to ABA during seed germination. Conversely, DRT111 over-expressing plants show ABA-hyposensitive seed germination. RNAseq experiments show that in dry seeds, DRT111 controls expression and splicing of genes involved in osmotic-stress and ABA responses, light signaling, and mRNA splicing, including targets of ABSCISIC ACID INSENSITIVE3 (ABI3) and PHYTOCHROME INTERACTING FACTORs (PIFs). Consistently, expression of the germination inhibitor SOMNUS, induced by ABI3 and PIF1, is up-regulated in imbibed seeds of drt111-2 mutants. Together, these results indicate that DRT111 controls sensitivity to ABA during seed development, germination, and stomatal movements, and integrates ABA-and light-regulated pathways to control seed germination.