The scope of flavin-dependent reactions and processes in the model plant Arabidopsis thaliana

Eggers, Reinmar Stefan; Jammer, Alexandra; Jha, Shalinee; Kerschbaumer, Bianca; Lahham, Majd; Strandback, Emilia Sofia; Toplak, Marina; Wallner, Silvia; Winkler, Andreas; Macheroux, Peter

doi:10.1016/j.phytochem.2021.112822

Cited by 24 publications

(28 citation statements)

References 298 publications

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“…The wll1 mutant phenotype could be rescued by FAD supplementation (Figure 2g, h), suggesting that the wll1 phenotype results from FAD deficiency. In plants, FAD and FMN play a vital role in the functions of numerous flavoenzymes involved in many processes (Eggers et al., 2021; Schall et al., 2020). Hence, it was not surprising that riboflavin deficiency for the production of FAD and FMN could impair flavin homeostasis and cause multisystem dysfunction during plant growth and development.…”

Section: Discussionmentioning

confidence: 99%

“…Finally, riboflavin synthase (RS) converts two molecules of 6,7‐dimethyl‐8‐ribityllumazine into riboflavin and ARP. Riboflavin can be further converted into FMN and FAD (Eggers et al., 2021; Fischer and Bacher, 2011; Schall et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Riboflavin, also known as vitamin B2, is the precursor of two essential cofactors, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), which serve as cofactors for up to 2% of all enzymes, and participate in numerous biological processes such as circadian clock regulation (Fitzpatrick and Noordally, 2021), nitrogen fixation (Macheroux et al., 2011) and hormone metabolism (Eggers et al., 2021; Schall et al., 2020). All plants and fungi, as well as most bacteria, produce riboflavin (Liu et al., 2020), and the riboflavin biosynthesis pathway is well studied (Liu et al., 2020; Sa et al., 2016).…”

Section: Introductionmentioning

confidence: 99%

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WHITE AND LESION‐MIMIC LEAF1, encoding a lumazine synthase, affects reactive oxygen species balance and chloroplast development in rice

Ren

et al. 2021

The Plant Journal

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The riboflavin derivatives flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) are essential cofactors for enzymes in multiple cellular processes. Characterizing mutants with impaired riboflavin metabolism can help clarify the role of riboflavin in plant development. Here, we characterized a rice (Oryza sativa) white and lesion-mimic (wll1) mutant, which displays a lesion-mimic phenotype with white leaves, chlorophyll loss, chloroplast defects, excess reactive oxygen species (ROS) accumulation, decreased photosystem protein levels, changes in expression of chloroplast development and photosynthesis genes, and cell death. Map-based cloning and complementation test revealed that WLL1 encodes lumazine synthase, which participates in riboflavin biosynthesis. Indeed, the wll1 mutant showed riboflavin deficiency, and application of FAD rescued the wll1 phenotype. In addition, transcriptome analysis showed that cytokinin metabolism was significantly affected in wll1 mutant, which had increased cytokinin and d-aminolevulinic acid contents. Furthermore, WLL1 and riboflavin synthase (RS) formed a complex, and the rs mutant had a similar phenotype to the wll1 mutant. Taken together, our findings revealed that WLL1 and RS play pivotal roles in riboflavin biosynthesis, which is necessary for ROS balance and chloroplast development in rice.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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WHITE AND LESION‐MIMIC LEAF1, encoding a lumazine synthase, affects reactive oxygen species balance and chloroplast development in rice

Ren

et al. 2021

The Plant Journal

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“…GULLO2 attracted our attention because it oxidizes L-gulono-1,4 γ-lactone into ASC, with H 2 O 2 as by-product; current knowledge is, however, that plants synthesize ASC, in vivo, solely via the D-mannose/L-galactose pathway and that the oxidation of L-gulono-1,4 γ-lactone by GULLO as the last ASC biosynthetic step, occurs in animal cells and not in plants (Smirnoff, 2018). To date, no physiological role has been assigned, in vivo, to GULLO2 (Eggers et al, 2021;Maruta et al, 2010); however, unpublished findings obtained so far in our research group, by using two independent A. thaliana gullo2 mutants would suggest its involvement in the reduction step of Fe(III) into Fe(II) in developing embryos (Murgia and coworkers, unpublished observations).…”

Section: ) Seed Fe Loading: When Where and Howmentioning

confidence: 99%

Plant iron nutrition: the long road from soil to seeds

Murgia

Marzorati

Vigani

et al. 2021

Journal of Experimental Botany

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Iron (Fe) is an essential plant micronutrient since photosynthesis, respiration, the scavenging of reactive oxygen species and many other cellular processes depend on adequate Fe levels. Nonetheless, non-complexed Fe ions can be dangerous for cells, as they can act as a pro-oxidant. Therefore, plants possess a complex homeostatic control system for safely taking up Fe from the soil, transporting it to the various cellular destinations and for its subcellular compartmentalization. At the end of the plant’s life cycle, maturing seeds are loaded with the required amount of Fe for germination and early seedling establishment. In this review, we discuss recent findings on how the microbiota in the rhizosphere influence and interact with the strategies adopted by plants to take up iron from the soil. We also focus on the process of seed loading with Fe and take into account the Fe metabolism in wild crops’ relatives. These aspects of plant Fe nutrition can represent promising avenues for a better comprehension of the long road of Fe from soil to seeds.

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“…To expand their applicability, it is essential to investigate the flavoprotein content and diversity in different organisms. So far, detailed information of flavoproteomes has only been reported in Homo sapiens , Saccharomyces cerevisiae , and Arabidopsis thaliana ( 5 , 6 , 34 , 35 ), to which has to be added a comparative analysis in a bunch of archaeal, eubacterial, protozoan, and eukaryotic genomes ( 7 ). These studies indicate that whereas some organisms depend heavily on flavin-dependent activities, others maintain a minimum of flavoproteins.…”

Section: Introductionmentioning

confidence: 99%

Mining the Flavoproteome of Brucella ovis, the Brucellosis Causing Agent in Ovis aries

et al. 2022

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The scope of flavin-dependent reactions and processes in the model plant Arabidopsis thaliana

Cited by 24 publications

References 298 publications

WHITE AND LESION‐MIMIC LEAF1, encoding a lumazine synthase, affects reactive oxygen species balance and chloroplast development in rice

WHITE AND LESION‐MIMIC LEAF1, encoding a lumazine synthase, affects reactive oxygen species balance and chloroplast development in rice

Plant iron nutrition: the long road from soil to seeds

Mining the Flavoproteome of Brucella ovis, the Brucellosis Causing Agent in Ovis aries

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