The Scientific Work of Theodor Förster: A Brief Sketch of his Life and Personality

Kramer, Horst E. A.; Fischer, Peter

doi:10.1002/cphc.201000733

Cited by 15 publications

(13 citation statements)

References 22 publications

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“…2-Naphthol is one of the earliest fluorescent photoacids ever discovered and has been thoroughly studied both experimentally and theoretically. , The p K a value of 2-naphthol drops by ∼7 units (from 9.5 to 2.8) upon photoexcitation. It was later found that the isomer 1-naphthol (Scheme ) was an even stronger photoacid with a p K a * of about 0 .…”

Section: Fluorescein and Green Fluorescent Protein (Gfp): The Preamblementioning

confidence: 99%

“…Many fluorescent molecules contain a hydroxyaromatic moiety. Prominent examples include fluorescein, which is a reliable choice of fluorescent labels for staining biological specimens; 1- and 2-naphthol, which are archetypal photoacids that have inspired the development of many more for achieving temporal and spatial control of proton-driven processes; and the light-emitting core of green fluorescent protein (GFP), which is a genetically encoded illuminator of subcellular targets and processes . The neutral and anion forms of these dyes differ in their emission wavelengths and quantum yields, with the anion often as the dominant emitter that characterizes the fluorescence.…”

Section: Introductionmentioning

confidence: 99%

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Hydroxyaromatic Fluorophores

et al. 2021

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Many fluorophores that are widely used in analytical biochemistry and in biological microscopy contain a hydroxyaromatic component. One could also find fascinating chemistries of hydroxyaromatic dyes, especially those capable of excited state proton transfer (ESPT) to produce dual emission, in the literature of materials and physical chemistry. The ESPT-capable compounds have attracted interest based on their fundamental intellectual values in molecular photophysics and their potential utilities as light emitters in organic light-emitting diodes (LEDs) or fluorescent sensors. The hydroxyaromatic dyes could undergo either intra-or intermolecular proton transfer in either electronic ground or excited states. Although having long been applied for various purposes, some of their absorption and emission properties have not always been clearly described because of the insufficient attention given to proton transfer equilibria in either the ground or excited state and the challenges in computationally modeling the true emitters of these dyes under any given conditions. In this article, an attempt is made to summarize the spectroscopic properties of a few common hydroxyaromatic dyes that have been studied for both fundamental and practical purposes, with the help from quantum chemical calculations of the absorption and emission energies of these dyes in neutral and anion forms. The goal of this article is to provide readers some clarity in the optical properties of these compounds and the tools to understand and to predict the photon-initiated behaviors of hydroxyaromatic fluorophores.

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Section: Fluorescein and Green Fluorescent Protein (Gfp): The Preamblementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hydroxyaromatic Fluorophores

et al. 2021

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“…Förster resonance energy transfer (FRET) is a process in which a donor molecule in excited state transfers its excitation energy through dipole-dipole coupling [1] to an acceptor fluorophore, when the two are brought into close proximity (typically less than 10 nm) [2], [3]. Upon excitation at a characteristic wavelength the energy absorbed by the donor is transferred to the acceptor, which in turn emits the energy.…”

Section: Introductionmentioning

confidence: 99%

Time-Resolved FRET -Based Approach for Antibody Detection – A New Serodiagnostic Concept

et al. 2013

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Förster resonance energy transfer (FRET) is a phenomenon widely utilized in biomedical research of macromolecular interactions. In FRET energy is transferred between two fluorophores, the donor and the acceptor. Herein we describe a novel approach utilizing time-resolved FRET (TR-FRET) for the detection of antibodies not only in a solution-phase homogenous assay but also in single- and two-step solid-phase assays. Our method is based on the principle that the Y-shaped immunoglobulin G molecule is able to simultaneously bind two identical antigen molecules. Hence, if a specific IgG is mixed with donor- and acceptor-labeled antigens, the binding of antigens can be measured by TR-FRET. Using donor- and acceptor-labeled streptavidins (SAs) in conjunction with a polyclonal and a monoclonal anti-SA antibody we demonstrate that this approach is fully functional. In addition we characterize the immune complexes responsible for the TR-FRET signal using density gradient ultracentrifugation and solid-phase immunoassays. The homogenous TR-FRET assay described provides a rapid and robust tool for antibody detection, with a wide potential in medical diagnostics.

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“…Walk into a symposium or platform talk at the Biophysical Society annual meetings, and there is a good possibility that Förster resonance energy transfer (FRET) is the technique being employed. This year celebrates the 65th year since the phenomenon, involving non-radiative energy transfer between two dipoles, was first described by Theodore Förster . Excellent reviews on FRET can be found elsewhere, − but very briefly, the technique is used as a molecular ruler that determines distance between two points in a biological system (in the range of 1–10 nm), each labeled with a different fluorescent probe.…”

Section: Biophysicists Like To Fret One Molecule At a Timementioning

confidence: 99%

Biophysics at the Cutting Edge: A Report from the 55th Annual Meeting of the Biophysical Society

Pal

2011

ACS Chem. Biol.

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T he 55th Annual Meeting of the Biophysical Society held at Baltimore in early March was attended by over 6000 scientists from over 20 different nations. The diversity in nationality was matched by the diversity of science presented at the conference. Over the course of 5 days, more than 30 symposia and over 3000 short platform sessions and posters presented a stunning array of biophysical science. To convey all that was presented is obviously impractical. This article will attempt to cover some of the more cutting-edge research, especially on those topics that are related to chemical biology. Also, rather than describing the talks in chronological order as they happened, the article is arranged under various categories based on broad research topics. Emphasis is also on various techniques, though the biological insights gained by each method are discussed briefly.

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The Scientific Work of Theodor Förster: A Brief Sketch of his Life and Personality

Cited by 15 publications

References 22 publications

Hydroxyaromatic Fluorophores

Hydroxyaromatic Fluorophores

Time-Resolved FRET -Based Approach for Antibody Detection – A New Serodiagnostic Concept

Biophysics at the Cutting Edge: A Report from the 55th Annual Meeting of the Biophysical Society

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