The Science of Plant Tissue Culture as a Catalyst for Agricultural and Industrial Development in an Emerging Economy

Aladele, S. E.; Okere, AU; Jamaldinne, E.; Lyam, Paul Terwase; Fajimi, O; Adegeye, A.J.; Zayas, Carlos M.

doi:10.5772/51843

Cited by 2 publications

(2 citation statements)

References 36 publications

(30 reference statements)

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“…NaDCC was effective against Pseudomonas, Xanthomonas, and Actinomycetes. Samples were analysed for the ef icacy of NaDCC with explants that were heavily contaminated with (Coates, 1996) Enterococcus faecalis (Heling et al, 2001) Lactobacillus sp (Guiteras and Schmelkes, 1934) Staphylococcus sp (Bloom ield and Uso, 1985;Proto et al, 2016) Streptococcus mutans (Heling et al, 2001) Streptococcus salivarius (Heling et al, 2001) Streptococcus sobrinus (Heling et al, 2001) Gram-negative Acinetobacter sp (Abreu et al, 2013) Campylobacter jejuni (Clasen and Edmondson, 2006) Enterobacteria sp (Abreu et al, 2013) Escherichia coli (Clasen and Edmondson, 2006;Proto et al, 2016) Pseudomonas aeruginosa (Lantagne et al, 2010) Pseudomonas sp (D' Auria et al, 1989) Salmonella dysenteriae (Clasen and Edmondson, 2006). Vibrio cholerae (Lantagne et al, 2010) Yersinia enterocolitica (Clasen and Edmondson, 2006) Fungus Aspergillus sp (Proto et al, 2016;Staniszewska, 2004).…”

Section: Potential Advantages Of Nadcc In Plant Tissue Culturementioning

confidence: 99%

“…However, substantial electricity cost is one of the critical factors that negate the popularisation of plant tissue culture-based enterprise (Pożoga et al, 2019). Plant tissue culture has the potential to be a catalyst for the development in both the agricultural and industrial sectors in the emerging economy (Aladele et al, 2012). Therefore a viable chemical method of sterilisation is the demand of the time for successful implementation and economic feasibility.…”

Section: Introductionmentioning

confidence: 99%

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Sodium Dichloroisocyanurate: An eco-friendly chemical alternative for media autoclaving and explant sterilisation in plant tissue culture

Shetty¹,

Narasimhan²

2021

ijrps

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Autoclaving nutrient media is still considered as the optimum mode of sterilisation in plant cell and tissue culture. During the process steam under high pressure is maintained at 120 degrees Celsius, 15 psi for 15-20 minutes in a chamber, optimised to kill all possible microbial life forms. But the disadvantages related to the process of autoclaving are plentiful. They are, decrease in the media pH, salt precipitation, agar depolymerisation, carbohydrate hydrolysis, volatile obliteration and necessity of the infrastructure investment. Requirements of additional resources (time, human resources, electrical energy) have forced the lookout for a more viable alternative, that is, chemical sterilisation. The use of Sodium dichloroisocyanurate (NaDCC) is a useful alternative for media and explant sterilisation. NaDCC is stable, water-soluble, non-toxic and easy to use at room temperature, does not have any environmental hazards and is not phytotoxic. The use of NaDCC as a disinfectant has been documented well concerning water sterilisation, surface sterilisation and also as a broad spectrum disinfecting agent. Disinfecting property of NaDCC is due to the hydrolytic release of chlorine, and this can be utilised for sterilisation of media and explants in plant tissue culture. NaDCC is a useful alternative for autoclaving at a concentration range of 0.05 to 1.0 g/l. However, only a few reports are available for its use as a sterilising agent for media and explants for in vitro cultures of plants. This paper discusses and reviews the possibility of establishing NaDCC as an active agent for explant sterilisation and as a viable alternative to medium sterilisation through autoclaving.

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