The Science of Cryobiology

Mullen, Steven F.; Critser, John K.

doi:10.1007/978-0-387-72293-1_7

Cited by 41 publications

(27 citation statements)

References 134 publications

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“…Cryopreservation is the process of preserving the viability of cells and tissues by freezing and storing them at the subzero temperatures below which biochemical reactions do not occur [20]. During freezing to such low temperatures cells can be subjected to damage, termed freezing injury.…”

Section: Introductionmentioning

confidence: 99%

Intracellular ice formation in confluent monolayers of human dental stem cells and membrane damage

2010

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Dental pulp stem cells (DPSCs) are of interest to researchers and clinicians due to their ability to differentiate into various tissue types and potential uses in cell-mediated therapies and tissue engineering. Currently DPSCs are cryopreserved in suspension using Me(2)SO. However, preservation as two and three dimensional constructs, along with the elimination of toxic Me(2)SO, may be required. It was shown that intracellular ice formation (IIF), lethal to cells in suspensions, may be innocuous in cell monolayers due to ice propagation between cells through gap junctions that results in improved post-thaw recovery. We hypothesized that innocuous IIF protects confluent DPSC monolayers against injury during cryopreservation. The objective was to examine the effects of IIF on post-thaw viability of both confluent monolayers and suspensions of DPSCs. Confluent DPSC monolayers were assessed for the expression of gap junction protein Connexin-43. IIF was induced on the cryostage and in the methanol bath at different subzero temperatures. Membrane integrity and colony-forming ability were assessed post-thaw. Confluent DPSC monolayers expressed Connexin-43. In cell suspensions, 85.9+/-1.7% of cells were damaged after 100% IIF. In cell monolayers, after 100% IIF, only 25.5+/-5.5% and 14.8+/-3.3% of cells were damaged on the cryostage and in the methanol bath respectively. However, DPSC monolayers exposed to 100% IIF showed no colony-forming ability. We conclude that confluent monolayers of DPSCs express the gap junction-forming protein Connexin-43 and upon IIF retain membrane integrity, however lose the ability to proliferate.

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Section: Introductionmentioning

confidence: 99%

Intracellular ice formation in confluent monolayers of human dental stem cells and membrane damage

2010

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“…Cryopreservation is the successful long-term maintenance of cellular or tissue biological function at low temperatures, suppressing metabolism and biochemical reactions, effectively ceasing “biological time” (Mullen and Critser, 2007). Although ultra-low temperatures offer long-term preservation, cryo-injuries such as ice formation-induced mechanical stress and freeze concentration-induced physiochemical deviation from physiological states are inevitable (Gao and Crister, 2000; Mazur, 2004; Mazur et al, 1972).…”

Section: Fundamentals Of Cryopreservationmentioning

confidence: 99%

“…The inverted ‘U’ shaped relationship between cell survival and cooling (Figure 1A) has been summarized in the literatures prior to understanding the mechanism behind it (Acker, 2007; Mazur, 1984, 2004; Mazur et al, 1972; Mullen and Critser, 2007). During slow freezing cryopreservation, cells may suffer injuries caused by deviations of the extra- and intracellular solutions from the physiological environment and extra- and intracellular ice formation (Figure 1B), as summarized by the “two-factor hypothesis” offered by Mazur (Mazur, 1970, 1984).…”

Section: Fundamentals Of Cryopreservationmentioning

confidence: 99%

Microfluidics for cryopreservation

Zhao

2017

Biotechnology Advances

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Cryopreservation has utility in clinical and scientific research but implementation is highly complex and includes labor-intensive cell-specific protocols for the addition/removal of cryoprotective agents and freeze-thaw cycles. Microfluidic platforms can revolutionize cryopreservation by providing new tools to manipulate and screen cells at micro/nano scales, which are presently difficult or impossible with conventional bulk approaches. This review describes applications of microfluidic chips in cell manipulation, cryoprotective agent exposure, programmed freezing/thawing, vitrification, and in situ assessment in cryopreservation, and discusses achievements and challenges, providing perspectives for future development.

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“…To date, genome cryobanking via oocytes has been much less attractive than sperm and embryo banking in almost all biomedically-important species [89,90]. This is mostly because of limitations regarding the number of oocytes obtained from mammalian species as well as low success in survival rate after freezing and thawing.…”

Section: Cryopreservationmentioning

confidence: 99%

“…Although adequate means for the cryopreservation of preimplantation embryos are available for the laboratory mouse and rats [33], methods for cryopreserving oocytes for many mammalian species are generally less successful. Mature oocytes have several, unique characteristics contributing to this recalcitrance [90]. The large intracellular water volume, cortical granules, and meiotic spindle all make them sensitive to the stresses imposed during freezing.…”

Section: Cryopreservationmentioning

confidence: 99%

Genome resource banking of biomedically important laboratory animals

Ağca

2012

Theriogenology

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Genome resource banking (GRB) is the systematic collection, storage, and re-distribution of biomaterials in an organized, logistical, and secure manner. Genome cyrobanks usually contain biomaterials and associated genomic information essential for progression of biomedicine, human health, and research. In that regard, appropriate genome cryobanks could provide essential biomaterials for both current and future research projects in the form of various cell types and tissues, including sperm, oocytes, embryos, embryonic or adult stem cells, induced pluripotent stem cells, and gonadal tissues. In addition to cryobanked germplasm, cryobanking of DNA, serum, blood products, and tissues from scientifically, economically and ecologically important species has become a common practice. For revitalization of the whole organism, cryopreserved germplasm in conjunction with assisted reproductive technologies (ART), offer a powerful approach for research model management, as well as assisting in animal production for agriculture, conservation, and human reproductive medicine. Recently, many developed and developing countries have allocated substantial resources to establish genome resources banks which are responsible for safeguarding scientifically, economically and ecologically important wild type, mutant and transgenic plants, fish, and local livestock breeds, as well as wildlife species. This review is dedicated to the memory of Dr. John K. Critser, who had made profound contributions to the science of cryobiology and establishment of genome research and resources centers for mice, rats and swine. Emphasis will be given to application of GRBs to species with substantial contributions to the advancement of biomedicine and human health.

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The Science of Cryobiology

Cited by 41 publications

References 134 publications

Intracellular ice formation in confluent monolayers of human dental stem cells and membrane damage

Intracellular ice formation in confluent monolayers of human dental stem cells and membrane damage

Microfluidics for cryopreservation

Genome resource banking of biomedically important laboratory animals

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