The SCID mouse: an experimental model for endometriosis

Abstract

The purpose of this study was to validate the suitability of the severe combined immunodeficient (SCID) mouse as an experimental model for endometriosis, by defining the morphological and histological features of induced endometrial implants, and characterizing specific biochemical properties of these implants. Human secretory endometrial tissues were injected into the peritoneal cavity of SCID/SCID CB17 mature female mice. Successful peritoneal implantation was observed in 55 of 57 (96.5%) SCID mice and consisted of circumscribed elevated nodules. Haematoxylin-eosin staining of implanting lesions demonstrated the presence of endometrial glandular tissue in a mixed background of stromal and inflammatory cells. When progesterone was administered to mice, epithelial glands underwent well-defined secretory changes. Immunohistochemical analysis using polyclonal human pan-cytokeratin antibodies demonstrated selective positive staining in the glandular epithelium of the human implants with none in the surrounding stroma. In-situ hybridization analysis using complement component 3 cDNA radiolabelled riboprobes yielded significantly more intense signals in glands compared to stroma. As human endometrial implants in SCID mice were shown to retain specific histological, functional and biochemical properties, we conclude that the SCID mouse is an attractive animal model for the study of endometriosis.

Memy I: a novel murine model for uterine leiomyoma using adenovirus-enhanced human fibroid explants in severe combined immune deficiency mice

“…10 It was obvious in the H&E-stained sections that the xenografts are not necrotic or fibrotic, and vascularization was evident because of the presence of small vessels and red blood cells (Figure 2). Thus, our results confirmed that pretreatment with both Ad-COX-2 and Ad-VEGF-A provided a means of satisfactory tissue homeostasis with sustained proliferation and minimal apoptosis that were similar to the results of the matched control leiomyoma tissue.…”

“…10 Humantissues serially passed in thesemice were shown to retain their morphologic and biochemical characteristics. 10 The aim of our study was to develop a reliable in vivo model of human uterine leiomyoma by using SCID mice implanted with human leiomyoma explants that were enhanced by transfection with an adenovirus carrying COX-2 and/or VEGF-A. …”

“…9 Severe combined immunodeficient (SCID) mice possess a combined congenital deficiency in T-and B-lymphocyte function and have been shown to host various heterotransplants successfully, as they cannot elicit humoral and cellular immune responses and, therefore, cannot reject transplanted nonautologous tissues. 10 Humantissues serially passed in thesemice were shown to retain their morphologic and biochemical characteristics. 10 The aim of our study was to develop a reliable in vivo model of human uterine leiomyoma by using SCID mice implanted with human leiomyoma explants that were enhanced by transfection with an adenovirus carrying COX-2 and/or VEGF-A.…”

Curcumin Is Efective In Improving Folliculogenesis Profile And Oo-cytes Quality In Vitro In Ectopic Endometriosis Mouse (Mus muscu-lus) Models

“…Awwad et al, (1999) had successfully performed the implantation of endometriosis in mice (Mus musculus) with a success rate of 96.5%. We, therefore, investigated the effectiveness of curcumin in endometriosis treatment.…”

“…Then, estrogen was injected intramuscularly in femoral biceps muscles on day 1 and 5 after the injection of female treatment endometrium. Thereafter, implantation of endometrial tissue was done on day-1 by injecting endometrial isolate intraperitoneally as much as 0.1 cc, equivalent to 0.6 grams of wet endometrial tissue (Awwad et al, 1999).…”