The SCID-hu mouse: murine model for the analysis of human hematolymphoid differentiation and function

Abstract

The study of human hematopoietic cells and the human immune system is hampered by the lack of a suitable experimental model. Experimental data are presented showing that human fetal liver hematopoietic cells, human fetal thymus, and human fetal lymph node support the differentiation of mature human T cells and B cells after engraftment into mice with genetically determined severe combined immunodeficiency. The resultant SCID-hu mice are found to have a transient wave of human CD4+ and CD8+ T cells and human IgG (immunoglobulin G) in the peripheral circulation. The functional status of the human immune system within this mouse model is not yet known.

Development of monoclonal antibodies for analyzing immune and hematopoietic systems of common marmoset

“…For generating such humanized mice, severe combine immunodeficient (SCID), nonobese diabetic (NOD)-SCID, RAG2-null, and NOD/shi-SCID/IL-2Rg null (NOG) mice have been used as recipients because they each lack their own immunity, to varying degrees, because of gene mutation/deficiency [6][7][8]. On the other hand, human cord blood cells, lymphoid tissues/cells, or leukemic cells have been engrafted as donors into such mice [9][10][11]. Reconstitution of the human immune system in mice has been successful to varying extents, depending on both the type of recipient mouse and donor cells.…”

Stem Cells, Hematopoiesis and Lineage Tracing: Transplantation-Centric Views and Beyond

“…Pioneering work exploited mice with a spontaneous mutation ( Prkdc scid ) that leads to an absence of functional murine B and T cells. The SCID model was successfully used to engraft human PB leukocytes ( Mosier et al, 1988 ), fetal tissues ( McCune et al, 1988 ), and BM cells ( Kamel-Reid and Dick, 1988 ; Lapidot et al, 1992 ). Further refinement of recipient strains involved backcrossing of the Prkdc scid mutation onto the nonobese diabetic (NOD) strain ( Shultz et al, 1995 ).…”

The Zebrafish model in dermatology: an update for clinicians

“…As an example, patient-derived cancer cell xenotransplantation (PDX) could help to overcome treatment resistance due to added mutations in tumor cells, by means of large-scale drug (small molecules) screening. This process is not as easily achievable in murine models as it may be in non-mammalian models such as fish, since recipient immunosuppression is required for PDX in mice [ 61 – 63 ].…”