The Sas3p and Gcn5p histone acetyltransferases are recruited to similar genes

Rosaleny, Lorena E.; Ruiz-García, Ana Belén; Garcı́a-Martı́nez, José; Pérez-Ortín, José E.; Tordera, Vicente

doi:10.1186/gb-2007-8-6-r119

Cited by 42 publications

(55 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our findings demonstrate that GAS1 has striking yet distinct genetic interactions with genes encoding the lysine acetyltransferases Gcn5 and Sas3, which themselves are synthetically lethal and have overlapping nucleosomal substrates (Howe et al 2001) and genome-wide localization patterns (Rosaleny et al 2007). Whereas the gas1 gcn5 double mutant is dead, there is mutual suppression of specific phenotypes in the gas1 sas3 strain.…”

Section: Discussionmentioning

confidence: 89%

“…In addition to the synthetic lethality, Gcn5 and Sas3 have overlapping sites of genomic localization (Rosaleny et al 2007) and share nucleosomal substrates (Howe et al 2001). Based on the similarities between Gcn5 and Sas3, we chose to analyze the gas1 sas3 double mutant to determine if the synthetic lethality observed with gas1 gcn5 was gene specific.…”

Section: Resultsmentioning

confidence: 99%

“…Although Sas3 has often been considered to be largely functionally redundant with Gcn5, previous research indicated that Sas3 can disrupt Gcn5-based acetylation of H3K14 at distinct genomic loci (Rosaleny et al 2007). They may also compete during other dynamic processes.…”

Section: Distinct Functions For Gcn5 and Sas3mentioning

confidence: 99%

“…Using a sas3 allele with diminished function, it was found that both Gcn5 and Sas3 play a role in cell cycle regulation, with decreased Sas3 activity coupled with deletion of GCN5 leading to G 2 /M arrest (Howe et al 2001). Loss of SAS3 leads to a decrease of H3K14Ac, primarily at genes involved in cell cycle regulation and cell division (Rosaleny et al 2007). Sas3 physically interacts with Chk1 (Liu et al 2000), a Mec1 DNA damage pathway effector kinase and Dpb4, which regulates DNA replication and telomere silencing (Tackett et al 2005).…”

Section: Distinct Functions For Gcn5 and Sas3mentioning

confidence: 99%

“…Gcn5 and Sas3 share nucleosomal H3 targets (reviewed in Lafon et al 2007) and deletion of both GCN5 and SAS3 is synthetically lethal (Howe et al 2001). Further, both Gcn5 and Sas3 are recruited to similar genomic regions (Rosaleny et al 2007). Whereas Gcn5 has been studied extensively, less is known about Sas3, due in part to the functional overlaps with Gcn5 as well as the limited independent phenotypes defined for SAS3 mutants.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Unexpected Function of the Glucanosyltransferase Gas1 in the DNA Damage Response Linked to Histone H3 Acetyltransferases in Saccharomyces cerevisiae

Eustice

Pillus

2014

Genetics

View full text Add to dashboard Cite

Chromatin organization and structure are crucial for transcriptional regulation, DNA replication, and damage repair. Although initially characterized in remodeling cell wall glucans, the b-1,3-glucanosyltransferase Gas1 was recently discovered to regulate transcriptional silencing in a manner separable from its activity at the cell wall. However, the function of Gas1 in modulating chromatin remains largely unexplored. Our genetic characterization revealed that GAS1 had critical interactions with genes encoding the histone H3 lysine acetyltransferases Gcn5 and Sas3. Specifically, whereas the gas1 gcn5 double mutant was synthetically lethal, deletion of both GAS1 and SAS3 restored silencing in Saccharomyces cerevisiae. The loss of GAS1 also led to broad DNA damage sensitivity with reduced Rad53 phosphorylation and defective cell cycle checkpoint activation following exposure to select genotoxins. Deletion of SAS3 in the gas1 background restored both Rad53 phosphorylation and checkpoint activation following exposure to genotoxins that trigger the DNA replication checkpoint. Our analysis thus uncovers previously unsuspected functions for both Gas1 and Sas3 in DNA damage response and cell cycle regulation.

show abstract

Section: Discussionmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Distinct Functions For Gcn5 and Sas3mentioning

confidence: 99%

Section: Distinct Functions For Gcn5 and Sas3mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Unexpected Function of the Glucanosyltransferase Gas1 in the DNA Damage Response Linked to Histone H3 Acetyltransferases in Saccharomyces cerevisiae

Eustice

Pillus

2014

Genetics

View full text Add to dashboard Cite

show abstract

Combinatorial patterns of histone modifications in Saccharomyces.cerevisiae

Cui

Liang

Liu

2011

Yeast

View full text Add to dashboard Cite

Histone modification is an important subject of epigenetics that plays an intrinsic role in transcriptional regulation. It has been suggested that multiple histone modifications act in a combinatorial fashion to form a 'histone code'. In this study, the combinatorial patterns of histone modifications were studied by using a Bayesian network at the level of individual nucleosomes in S. cerevisiae. Our results indicated that there were 23 combinatorial patterns for 12 histone modifications investigated when a general Bayesian network was constructed. Meanwhile, different networks were also constructed for the genes with high transcript levels (H-network) and low transcript levels (L-network), respectively. Comparison among the general network, H-network and L-network illustrated four conserved combinations: H2BK16Ac → H3K4me3; H3K14Ac → H3K4me3; H2AK7Ac → H3K14Ac; and H4K12Ac → H3K18Ac. The detailed analysis for some combinations demonstrated that the combinations were ascribed to some histone-modifying enzymes.

show abstract