The Salt-Stress Signal Transduction Pathway That Activates the <i>gpx1</i> Promoter Is Mediated by Intracellular H2O2, Different from the Pathway Induced by Extracellular H2O2

Avsian-Kretchmer, Orna; Gueta-Dahan, Yardena; Lev‐Yadun, Simcha; Gollop, Rachel; Ben–Hayyim, Gozal

doi:10.1104/pp.104.041921

Cited by 80 publications

(59 citation statements)

References 73 publications

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“…At least two copies of a gene found in this study code for the PHGPX that was experimentally found to be responsible for salt-stress signaling in citrus (Holland et al, 1993;Avsian-Kretchmer et al, 2004).…”

Section: Glutathione Transferases and Glutathione Peroxidasesmentioning

confidence: 78%

Genes associated with hypersensitive response (HR) in the citrus EST database (CitEST)

et al. 2007

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Plants are continuously exposed to pathogen attack, but successful infection is rare because they protect themselves against pathogens using a wide range of response mechanisms. One of them is the hypersensitive response (HR), which is a form of cell death often associated with plant resistance to pathogen infection to prevent the spreadsebpg@cnpq.brsebpg@cnpq.br of the potential pathogen from infected to uninfected tissues. Cell death is activated by recognition of pathogen-derived molecules by the resistance (R) gene products, and is associated with the massive accumulation of reactive oxygen species (ROS), salicylic acid (SA), and other pro-death signals such as nitric oxide (NO). The analysis of the citrus EST (CitEST) database revealed the presence of putative genes likely to be involved in HR through their products, like metacaspases, lipoxygenases, phospholipases, pathogenesis-related proteins, glutathione transferases/peroxidases, enzymes involved in the phenylpropanoid pathway and in the formation and detoxification of ROS, as well as those involved in the formation and regulation of ion channels, SA and NO. By analysis of the EST database of Citrus, it was possible to identify several putative genes that code for key enzymes involved in HR triggering and also in plant defense against biotic and abiotic stress.Key words: otic and abiotic stress response, plant-pathogen incompatible interaction, programmed cell death, plant disease resistance.

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Section: Glutathione Transferases and Glutathione Peroxidasesmentioning

confidence: 78%

Genes associated with hypersensitive response (HR) in the citrus EST database (CitEST)

et al. 2007

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“…The synthesis of catalase in LECs was reported to be dependent on the concentration of H 2 O 2 [16]. It was also reported that H 2 O 2 activates the expression of glutathione peroxidase, an inactivator of H 2 O 2 [17]. Interestingly, during inflammatory colitis, an increase in glutathione peroxidase with a decrease in AQP8 was reported [18].…”

Section: Discussionmentioning

confidence: 99%

Immunolocation of Aquaporin 8 in Human Cataractous Lenticular Epithelial Cells

Hayashi

Fukuda

et al. 2017

Biomed Hub

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Purpose/Aim: Aquaporin 8 (AQP8) is a diffusion facilitator of hydrogen peroxide (H₂O₂) through cell membranes. The purpose of this study was to confirm and localize AQP8 in human lenticular epithelial cells (LECs). Materials and Methods: Lenticular anterior capsule samples, including LECs, were collected during cataract surgery of cataract patients after informed consent. The localization of AQP8 was detected by immunohistochemical staining using an antibody to AQP8. Real-time polymerase chain reaction (RT-PCR) was also used to determine the AQP8 mRNA expression levels. The PCR products were analyzed by gel electrophoresis following analyses of band density. Results: Immunohistochemical staining showed AQP8 was distributed throughout the whole area of the anterior capsulotomy. AQP8 labeling was observed surrounding and within the cytoplasm of LECs. RT-PCR and gel electrophoresis also revealed the presence of AQP8 mRNA in the lenticular anterior capsule. The results of immunohistochemical staining were comparable to those of RT-PCR and gel electrophoresis. Conclusions: The results of this study indicate the distribution of AQP8 in human LECs. This is the first investigation confirming the presence of AQP8 in human LECs.

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“…Changes in gene expression were correlated with speci fi c H 2 O 2 levels also induced by abiotic stress (Vandenabeele et al 2003(Vandenabeele et al , 2004Vanderauwera et al 2005 ) . In citrus, Avsian-Kretchmer et al ( 2004 ) reported that the promoter of the gpx1 gene (encoding for a glutathione peroxidase) responded speci fi cally to hydrogen peroxide. This was evidenced by the fact that ectopic expression of uidA gene under the control of gpx1 promoter was induced by abiotic elicitors (NaCl, sorbitol, mannitol, KCl, and, to a lower extent, by Na 2 SO 4 ) as well as by H 2 O 2 .…”

Section: Role Of Ros As Signaling Molecules and Interaction With Planmentioning

confidence: 99%

Antioxidant System in Citrus Under Nutrient Stress Conditions: Latest Developments

Arbona

2012

Advances in Citrus Nutrition

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Natural distribution of mineral nutrients in the earth crust is a crucial factor that determines the distribution of plant species favoring the spreading of species with speci fi c adaptations to nutrient de fi ciency or excess. Throughout years, humans have learned how to provide nutrients to poorly fertilized soils and, to some extent, how to regulate absorption of certain nutrients when these are present in excess in the substrate. However, this has led also to an overfertilization of certain areas therefore contaminating arable soils and aquifers. In plants, both excess and de fi cient fertilization induce alterations in metabolism growth and reproductive development. The extent to which the nutrient imbalance affects plant physiology depends on the speci fi c nutrient and the relative tolerance of the plant species. In addition, nutrients might also interact with each other, depending on their relative concentration, leading to nonexpected alterations in plants. For these reasons, nutrient imbalances are considered a source of abiotic stress in crop plants. As for other abiotic stress conditions such as salinity, soil waterlogging, heat, plants respond by modulating their photosynthetic metabolism and subsequently overproducing highly reactive oxidative subproducts as a secondary source of stress. In this sense, some of the macroscopic effects observed in plants subjected to nutritional imbalances (e.g., necrotic spots, chlorosis) could be a direct (or indirect) result of the production and accumulation of these substances. In this chapter, the effects of nutritional imbalances on oxidative stress are reviewed, focusing on citrus and providing examples on other crops or Arabidopsis thaliana . AbstractNutritional imbalances are important factors that determine the distribution of natural populations of plants. However, in crop plants, alterations in nutrient levels limit vegetative growth, development and ultimately affect yield and production quality. In citrus, this is a relevant aspect due to the economic importance of this fruit tree. As other abiotic stress factors, nutrient imbalances affect the redox state of the cells by altering normal electron fl ow through transport chains (chloroplastic and/or mitochondrial) or impairing reactive oxygen species scavenging ability of plants. In this chapter, the effects of macro-and micronutrient imbalances on citrus cell redox state are reviewed, paying special attention to the defense and detoxi fi cation mechanisms involved. In addition, the role of reactive oxygen species as signaling compounds alone or associated with plant hormones is discussed.

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The Salt-Stress Signal Transduction Pathway That Activates the gpx1 Promoter Is Mediated by Intracellular H2O2, Different from the Pathway Induced by Extracellular H2O2

Cited by 80 publications

References 73 publications

Genes associated with hypersensitive response (HR) in the citrus EST database (CitEST)

Genes associated with hypersensitive response (HR) in the citrus EST database (CitEST)

Immunolocation of Aquaporin 8 in Human Cataractous Lenticular Epithelial Cells

Antioxidant System in Citrus Under Nutrient Stress Conditions: Latest Developments

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