2018
DOI: 10.1186/s13072-018-0184-2
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The SAGA/TREX-2 subunit Sus1 binds widely to transcribed genes and affects mRNA turnover globally

Abstract: BackgroundEukaryotic transcription is regulated through two complexes, the general transcription factor IID (TFIID) and the coactivator Spt–Ada–Gcn5 acetyltransferase (SAGA). Recent findings confirm that both TFIID and SAGA contribute to the synthesis of nearly all transcripts and are recruited genome-wide in yeast. However, how this broad recruitment confers selectivity under specific conditions remains an open question.Results Here we find that the SAGA/TREX-2 subunit Sus1 associates with upstream regulatory… Show more

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Cited by 22 publications
(20 citation statements)
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“…The quantitatively similar behavior of SR and RA indicates that global mRNA stability was not appreciably different between the two yeast strains ( Fig EV4). Moreover, supportive of a general role for Mog1 in affecting the transcriptional rate and mRNA abundance, similar effects were observed for transcription of SAGAand TFIID-dominated genes ( Fig 3D), in agreement with the contribution of both SAGA and TFIID to the expression of nearly all yeast genes [55][56][57][58]. These findings led us to the conclusion that Mog1 has a genome-wide impact on RNAPII-transcribed mRNAs.…”
Section: Mog1 Absence Affects H2b Monoubiquitination-dependent Processupporting
confidence: 84%
“…The quantitatively similar behavior of SR and RA indicates that global mRNA stability was not appreciably different between the two yeast strains ( Fig EV4). Moreover, supportive of a general role for Mog1 in affecting the transcriptional rate and mRNA abundance, similar effects were observed for transcription of SAGAand TFIID-dominated genes ( Fig 3D), in agreement with the contribution of both SAGA and TFIID to the expression of nearly all yeast genes [55][56][57][58]. These findings led us to the conclusion that Mog1 has a genome-wide impact on RNAPII-transcribed mRNAs.…”
Section: Mog1 Absence Affects H2b Monoubiquitination-dependent Processupporting
confidence: 84%
“…The absence of DSS1-(I) and DSS1-(V) in the THP1-EYFP IP-MS might be because the proteins are short (70–100 amino acids) or are not an integral component of TREX-2. Sus1 in yeast is considered a linker protein that associates with both the SAGA complex and TREX-2 in co-regulating gene transcription and mRNA turnover 28 . We did not find Arabidopsis SUS1 (also called ENY2) from our IP-MS. A recent study showed that SUS1 associates with Arabidopsis SAGA but not with TREX-2 29 .…”
Section: Resultsmentioning
confidence: 99%
“…Genomic run-on (GRO) was performed as described in [8]. Their datasets are available at the Gene Expression Omnibus (GEO) with accession numbers: GSE29519, GSE57467 and GSE155372.…”
Section: Genomic Methodsmentioning
confidence: 99%