The saffron effects on expression pattern of critical self-renewal genes in adenocarcinoma tumor cell line (AGS)

Toxicity of Saffron Extracts on Cancer and Normal Cells: A Review Article

“…A number of mechanisms have been proposed for the anticancer activity of saffron and its components (Salomi et al, 1991;Nair et al, 1995;Moradzadeh et al, 2018). Saffron extract exerts its anti-cancer effects via RNA and DNA synthesis inhibition (Abdullaev and Frenkel, 1992;Abdullaev, 1994;Abdullaev, 2002;Tavakkol-Afshari et al, 2008;Patel et al, 2017), apoptosis induction (Tavakkol-Afshari et al, 2008;Samarghandian et al, 2013;Patel et al, 2017;Colapietro et al, 2019), self-renewal genes expression reduction (Akbarpoor et al, 2020), topoisomerase inhibition (Abdullaev, 2002;Lee and Park, 2003;Patel et al, 2017;Wang and DU, 2018), cell proliferation inhibition (Samarghandian et al, 2013;Patel et al, 2017;Colapietro et al, 2019), immune modulation (Khorasanchi et al, 2018), DNA fragmentation (Gezici, 2019), Metalloproteinase and Urokinase modulation (Festuccia et al, 2014), and cell growth reduction (Salomi et al, 1991;Bathaie et al, 2013;Makhlouf et al, 2016). Cancerous cells are more sensitive to the inhibitory effect of saffron on DNA, RNA and protein synthesis than healthy cells (Abdullaev and Frenkel, 1992;Tavakkol-Afshari et al, 2008).…”

“…Saffron extract at concentrations of 20, 40 and 100 μg/ml applied for 24, 48 and 72 hours reduced the expression self-renewal genes such as OCT4, KLF, SOX2, NANOG, and nucleostemin in gastric adenocarcinoma tumor cell line (Akbarpoor et al, 2020).…”

Evaluation of the Effect of Orlistatorlistat on Expression of OCT4, Nanog, SOX2, and KLF4 Genes in Colorectal Cancer SW40 Cell Line

“…Following the add 10 μL of 5 mg/ml MTT to each well, plates were incubated at 37 o C for 3.5 hours in the dark room, until the development of a purple precipitate was visible under the light microscope. Then 100 μL of DMSO (Dimethyl sulfoxide) was added to each well, and after 15 min, the absorbance was read at 570 (Karimabad et al, 2017;Akbarpoor et al, 2020).…”

Recent Advances on the Anticancer Properties of Saffron (Crocus sativus L.) and Its Major Constituents

“…Gastrointestinal cancer is amongst the most studied cancers with regard to the potential anticancer activity of saffron and its ingredients [ 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. Studies have shown that crocin can induce apoptotic cell death in colorectal cancer cells through p53 dependent and independent mechanisms [ 30 ].…”

“…Other studies support the finding that crocin can lead to an increase of the Bax/Bcl-2 ratio, activation of caspases, and also the reduction of the expression of genes such as OTC4, SOX2, NANOG, KLF4, and NUCLEOSTEMIN in AGS cells. These genes are known to regulate the cell cycle and self-regeneration in stem cells [ 34 ].…”