The safety of weekly plateletpheresis: effect on the donors' lymphocyte population

Koepke, J.A.; Parks, William M.; Goeken, James A.; Klee, George G.; Strauss, Ronald G.

doi:10.1046/j.1537-2995.1981.21181127485.x

Cited by 51 publications

(22 citation statements)

References 10 publications

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“…Although one subject (donor No.5) demonstrated an appreciable fall in circulating absolute lymphocyte counts as sociated with the series of lymphapheresis procedures, lymphocyte counts of the group as a whole were not significantly altered by the protocol used. Our results differ from those reported by Koepke et al [15] who reported a 22% decrease in the circulating lymphocyte count in normal blood donors following repeat plateletpheresis (10 proce dures over 12 weeks), and from Wright et al [5] who have reported significant lymphope nia following repeat lymphapheresis of pa tients with rheumatoid arthritis. The discre pancy between these reports and our results may relate to differences in the intensity and duration of lymphocyte removal since stud ies involving thoracic duct drainage, in…”

Section: Percent Of Lymphocytes Bearing Surface Immunoglobulin (Smlaicontrasting

confidence: 99%

Immune Function in Blood Donors Following Short-Term Lymphocytapheresis

Blanchette¹,

Dunne²,

Steele³

et al. 1985

Vox Sanguinis

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Lymphocyte losses occur during repeat plateletpheresis and/or leukapheresis procedures and are potentially harmful to normal host immune function. This concern prompted us to study the effect of repeat lymphocytapheresis (lymphapheresis) on the immune system of healthy blood donors. Following a total of six lymphapheresis procedures over a 12-day period with removal of a mean of 41.6 x 10^9 lymphocytes, there were no significant changes in donor (n = 5) absolute lymphocyte counts, quantitative immunoglobulin levels or immune response capability as assessed by the following in vitro assays: percent of E, EA, and EAC rosette-forming cells, the percent of surface membrane immunoglobulin bearing cells, and the blastogénie responses to stimulation with phytohemagglutinin, pokeweed mitogen and concanavalin A. The procedures were well-tolerated without complications. During a follow-up period of 18 months, significant increases in total white cell counts (but not absolute lymphocyte counts), and in quantitative immunoglobulin levels (IgG, IgM, and IgA) were observed. The median increases in immunoglobulin levels ranged from 27.5% for IgG to 44% for IgA. We conclude that lymphapheresis as described is relatively safe and our results are reassuring in relation to lymphocyte losses that may occur during repeat cytapheresis procedures of normal blood donors.

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Section: Percent Of Lymphocytes Bearing Surface Immunoglobulin (Smlaicontrasting

confidence: 99%

Immune Function in Blood Donors Following Short-Term Lymphocytapheresis

Blanchette¹,

Dunne²,

Steele³

et al. 1985

Vox Sanguinis

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“…Apheresis platelet donors in the United States currently donate up to 24 times in a rolling 12‐month period . In the 1980s, there was concern that frequent plateletpheresis might cause lymphocyte depletion in donors . However, this concern waned as apheresis technology improved …”

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confidence: 99%

CD4+ T‐cell lymphopenia in frequent platelet donors who have ceased platelet donation for at least 1 year

et al. 2019

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BACKGROUND We recently discovered that 30% of current frequent apheresis platelet donors in a study at our donor center had CD4+ counts below 200 cells/μL. How long CD4+ lymphopenia persists after ceasing plateletpheresis is unknown. Whether there are infectious or other complications in former frequent donors that could relate to CD4+ lymphopenia is also unknown. STUDY DESIGN AND METHODS We mailed a letter to former frequent apheresis platelet donors who had not donated platelets for at least 12 months. Frequent donation was defined as 20 to 24 plateletpheresis sessions in at least one 365‐day period starting in 2011. Donors who expressed interest in the study were contacted to schedule a study visit. Participants in the study provided a blood sample and completed a health questionnaire that included questions about opportunistic infections and malignancies. RESULTS Of 50 potential study candidates who were mailed a letter, 15 participated in the study. There were 2 participants with CD4+ counts below 200 cells/μL, one of whom had prior counts that documented a small improvement with cessation of plateletpheresis. Three participants had counts between 200 and 300 cells/μL. No study participant had a history of an opportunistic infection or a malignancy associated with immune dysregulation. CONCLUSION We detected CD4+ lymphopenia in former frequent apheresis platelet donors who had ceased platelet donation for more than 1 year. There was no evidence that the CD4+ lymphopenia predisposes to opportunistic infections or to malignancies associated with immune dysregulation.

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“…During a single plateletpheresis proce dure as many as 30% of the donor's circulat ing lymphocytes may be removed. Despite this, no reduction in peripheral blood lym phocytes occurs following the procedure [27,28]. A more prolonged single procedure with deliberate removal of about 9 x 1010 lym phocytes will induce a lymphocytopenia which recovers in 24 h [29].…”

Section: Discussionmentioning

confidence: 99%

“…In one, 10 new donors underwent weekly plateletpheresis for 10 weeks, at which time a 22% reduction had occurred. 50% of the donors showed a mild decrease in percentage of B cells, but T cells were unaffected [28], In the second study, after 5-9 leukapheresis procedures, donors were found to have a reduction in the percent of T cells but no alteration of in vitro reactivity in MLC or mitogen response [32], In neither study was the recovery time of the peripheral blood lymphocyte levels mentioned. Lymphocyte depletion and immunosuppression can cer tainly be induced for therapeutic purposes by thoracic duct drainage [33][34][35][36][37][38][39] or lymphocytapheresis of 2.5xlO10 cells per week using protocols specifically designed to re move T lymphocytes.…”

Section: Discussionmentioning

confidence: 99%

Long-Term Follow-Up of Donors Cytapheresed More Than 50 Times

Heal¹,

Horan²,

Schmitt³

et al. 1983

Vox Sanguinis

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11 volunteers who had donated white blood cells or platelets more than 50 times over a 5- to 9-year period were studied to determine whether any adverse consequences of many cytaphereses could be detected. Among the donors no significant differences were found in 18 hematological and biochemical parameters when compared to a group ofageand sex-matched nondonor controls. Despite extensive cumulative lymphocyte losses sustained by these donors, the ratio of T, B, helper and suppressor cells has been maintained within the normal range. No detrimental effects of multiple cytapheresis on the donors’ health has been demonstrated to date.

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The safety of weekly plateletpheresis: effect on the donors' lymphocyte population

Cited by 51 publications

References 10 publications

Immune Function in Blood Donors Following Short-Term Lymphocytapheresis

Immune Function in Blood Donors Following Short-Term Lymphocytapheresis

CD4+ T‐cell lymphopenia in frequent platelet donors who have ceased platelet donation for at least 1 year

Long-Term Follow-Up of Donors Cytapheresed More Than 50 Times

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