The Safety of Embryonic Stem Cell Therapy Relies on Teratoma Removal

Tang, Chad; Drukker, Micha

doi:10.18632/oncotarget.434

Cited by 12 publications

(7 citation statements)

References 8 publications

(12 reference statements)

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“…These results confirmed the formation of functional hepatocytes from the RCM1 hESC line, as has been reported with other hES cell lines (3,4). However, the study showed the presence of differentiation-resistant colonies within the eDH population, suggesting their unsuitability for transplantations, as the presence of contaminating ES cell colonies can result in teratoma formation following transplantation (14).…”

Section: Discussionsupporting

confidence: 89%

Polarisation and functional characterisation of hepatocytes derived from human embryonic and mesenchymal stem cells

et al. 2015

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Adult hepatocytes are polarised with their apical and basolateral membranes separated from neighbouring cells by tight junction proteins. Although efficient differentiation of pluripotent stem cells to hepatocytes has been achieved, the formation of proper polarisation in these cells has not been thoroughly investigated. In the present study, human embryonic stem cells (hESCs) and human mesenchymal stem cells (hMSCs) were differentiated to hepatocyte-like cells and the derived hepatocytes were characterised for mature hepatocyte markers. The secretion of hepatic proteins, expression of hepatic genes and the functional hepatic polarisation of stem cell-derived hepatocytes, foetal hepatocytes and the HepG2 hepatic cell line were evaluated and the different lines were compared. The results indicate that hESC-derived hepatocytes are phenotypically more robust and functionally more efficient compared with the hMSC-derived hepatocytes, suggesting their suitability for toxicity studies.

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Section: Discussionsupporting

confidence: 89%

Polarisation and functional characterisation of hepatocytes derived from human embryonic and mesenchymal stem cells

et al. 2015

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“…Among the first innovations here has been the use of embryonic stem cells (ESCs), whose employment presents the advantage of allowing the creation of 3D structures which resemble kidneys without the use of scaffolding, such totipotential cells being able to differentiate into renal cells such as tubules and podocytes [ 73 – 75 ]. Disadvantages, however, center not only around ethical concerns but also the possibility of teratoma formation [ 76 , 77 ]. As a result, research has focused more on differentiated autologous stem cells such as bone marrow derived from mesenchymal stem cells (MSCs), adipose derived stem cells (ADSCs), or amniotic fluid stem cells (AFSCs).…”

Section: Tissue Engineering and Regenerative Medicine As Kidney Replamentioning

confidence: 99%

Novel surgical techniques, regenerative medicine, tissue engineering and innovative immunosuppression in kidney transplantation

Nowacki¹,

Nazarewski²,

Kloskowski³

et al. 2016

aoms

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On the 60th anniversary of the first successfully performed renal transplantation, we summarize the historical, current and potential future status of kidney transplantation. We discuss three different aspects with a potential significant influence on kidney transplantation progress: the development of surgical techniques, the influence of regenerative medicine and tissue engineering, and changes in immunosuppression. We evaluate the standard open surgical procedures with modern techniques and compare them to less invasive videoscopic as well as robotic techniques. The role of tissue engineering and regenerative medicine as a potential method for future kidney regeneration or replacement and the interesting search for novel solutions in the field of immunosuppression will be discussed. After 60 years since the first successfully performed kidney transplantation, we can conclude that the greatest achievements are associated with the development of surgical techniques and with planned systemic immunosuppression.

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“…Pluripotent stem cells may form teratomas in vivo, and need to be differentiated into cell types that are unable to form these precancerous structures. This can be achieved after cell differentiation by selecting against residual pluripotent cell markers 77 .…”

Section: Tackling Reproducibility and Bias In Cell Therapymentioning

confidence: 99%

How to design preclinical studies in nanomedicine and cell therapy to maximize the prospects of clinical translation

2018

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The clinical translation of promising products, technologies and interventions from the disciplines of nanomedicine and cell therapy has been slow and inefficient. In part, translation has been hampered by suboptimal research practices that propagate biases and hinder reproducibility. These include the publication of small and underpowered preclinical studies, suboptimal study design (in particular, biased allocation of experimental groups, experimenter bias and lack of necessary controls), the use of uncharacterized or poorly characterized materials, poor understanding of the relevant biology and mechanisms, poor use of statistics, large between-model heterogeneity, absence of replication, lack of interdisciplinarity, poor scientific training in study design and methods, a culture that does not incentivize transparency and sharing, poor or selective reporting, misaligned incentives and rewards, high costs of materials and protocols, and complexity of the developed products, technologies and interventions. In this Perspective, we discuss special manifestations of these problems in nanomedicine and in cell therapy, and describe mitigating strategies. Progress on reducing bias and enhancing reproducibility early on ought to enhance the translational potential of biomedical findings and technologies.

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The Safety of Embryonic Stem Cell Therapy Relies on Teratoma Removal

Cited by 12 publications

References 8 publications

Polarisation and functional characterisation of hepatocytes derived from human embryonic and mesenchymal stem cells

Polarisation and functional characterisation of hepatocytes derived from human embryonic and mesenchymal stem cells

Novel surgical techniques, regenerative medicine, tissue engineering and innovative immunosuppression in kidney transplantation

How to design preclinical studies in nanomedicine and cell therapy to maximize the prospects of clinical translation

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