The Safe Recipient of SSC Transplantation Prepared by Heat Shock With Busulfan Treatment in Mice

Ma, Wenzhi; Wang, Jia; Gao, Wei-Jun; Hua, Jia

doi:10.1177/0963689718794126

Cited by 13 publications

(8 citation statements)

References 30 publications

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“…The use of a single heat shock exposure in emptying seminiferous tubules from germ cells was not effective; however, there was no significant difference between the two other groups. Considering lower side effects of heat shock plus testis busulfan injection model, it can be deemed as a practical method to prepare recipients of SSC transplantation (Ma, Wang, Gao, & Jia, ). In the other study, a single heat shock to scrotum at 43°C for 15 min (experimental group) decreased the weight of testis.…”

Section: Discussionmentioning

confidence: 99%

Hyperthermia versus busulfan: Finding the effective method in animal model of azoospermia induction

et al. 2019

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Animal models of azoospermia are very applicable when evaluating new treatment methods for research purposes. The present study aimed to compare azoospermia induction in mice using busulfan or hyperthermia. To do this, about 36 adult male mice (28–30 g) were included into three experimental groups randomly (n = 12): control, busulfan (injected by a single dose of 40 mg/kg busulfan intraperitoneally) and hyperthermia (exposure to a temperature of 43°C every other day for 5 weeks). Animals were preserved for 35 and 70 days following interventions and then were sacrificed for further evaluations. After 35 days, busulfan and hyperthermia groups revealed a significant decrease in the sperm count and weight of testis compared to the control group (p < .0001). In addition, after 70 days, sperm count and weight of testis in group busulfan showed a significant increase compared to group hyperthermia (p < .01). No significant difference was observed regarding the mortality of mice between busulfan and hyperthermia groups. In group busulfan, degenerative changes in the germinal epithelium were detected in some tubules, although in group hyperthermia, degenerative changes and complete depletion of all tubules were observed. Continuous hyperthermia is a more effective method in the induction of as animal model of azoospermia compared to the busulfan.

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Section: Discussionmentioning

confidence: 99%

Hyperthermia versus busulfan: Finding the effective method in animal model of azoospermia induction

et al. 2019

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“…We established a stable heat shock treatment scheme for in vitro-cultured SSCs. In our previous experiment, we treated the testes with a single heat shock treatment in a hot water bath at 43 °C for 15 min [9, 32]. Therefore, in this study, we treated SSCs cultured in vitro at 43 °C in a CO 2 incubator for different times, including 10 min, 15 min, 30 min, 45 min and 60 min.…”

Section: Discussionmentioning

confidence: 99%

High temperature suppressed SSC self-renewal through S phase cell cycle arrest but not apoptosis

et al. 2019

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Background High temperature has a very adverse effect on mammalian spermatogenesis and eventually leads to sub- or infertility through either apoptosis or DNA damage. However, the direct effects of heat stress on the development of spermatogonial stem cells (SSCs) are still unknown because SSCs are rare in the testes. Methods In the present study, we first used in vitro-cultured SSCs to study the effect of heat shock treatment on SSC development. Then, we used RNA-Seq analysis to identify new genes or signalling pathways implicated in the heat stress response. Results We found that 45 min of 43 °C heat shock treatment significantly inhibited the proliferation of SSCs 2 h after treatment but did not lead to apoptosis. In total, 17,822 genes were identified by RNA-Seq after SSC heat shock treatment. Among these genes, we found that 200 of them had significantly changed expression, with 173 upregulated and 27 downregulated genes. The number of differentially expressed genes in environmental information processing pathways was 37, which was the largest number. We screened the candidate JAK-STAT signalling pathway on the basis of inhibition of cell cycle progression and found that the JAK-STAT pathway was inhibited after heat shock treatment. The flow cytometry results further confirmed that heat stress caused S phase cycle arrest of SSCs. Conclusion Our results showed that heat shock treatment at 43 °C for 45 min significantly inhibited SSC self-renewal through S phase cell cycle arrest but not apoptosis.

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“…SSCT application in animal production also requires a maximal exclusion of endogenous SSCs to avoid production of offspring of endogenous origin. To address this issue, a variety of strategies for germline ablation to generate sterile recipients are available, such as chemotherapy [ 8 , 9 , 10 ], irradiation [ 81 , 82 ], heat shock treatment [ 83 , 84 ], or the use of genetically defective animals with congenital deletion of endogenous spermatogenesis [ 8 , 9 , 36 ].…”

Section: Preparation Of Recipientsmentioning

confidence: 99%

Spermatogonial Stem Cell Transplantation in Large Animals

Zhao

Wan

Zhang

et al. 2021

Animals

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Spermatogonial stem cell transplantation (SSCT) can restore male fertility through transfer of germline between donor and recipient males. From an agricultural perspective, SSCT could be an important next-generation reproductive and breeding tool in livestock production. Current SSCT approaches in large animals remain inefficient and many technical details need further investigation. This paper reviews the current knowledge on SSCT in large animals, addressing (1) donor spermatogonial stem cell (SSC) preparation, (2) recipient male treatment, and (3) SSC injection, homing, and detection. The major studies showing unequivocal evidence of donor SSC-derived spermatogenesis in large animals (mainly in livestock for breeding purpose) are summarized to discuss the current status of the field and future directions.

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The Safe Recipient of SSC Transplantation Prepared by Heat Shock With Busulfan Treatment in Mice

Cited by 13 publications

References 30 publications

Hyperthermia versus busulfan: Finding the effective method in animal model of azoospermia induction

Hyperthermia versus busulfan: Finding the effective method in animal model of azoospermia induction

High temperature suppressed SSC self-renewal through S phase cell cycle arrest but not apoptosis

Spermatogonial Stem Cell Transplantation in Large Animals

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