The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE).

Martínez‐Pastor, María Teresa; Marchler, Gabriele H.; Schüller, Christoph; Marchler‐Bauer, Aron; Ruis, Helmut; Estruch, Francisco

doi:10.1002/j.1460-2075.1996.tb00576.x

Cited by 979 publications

(1,031 citation statements)

References 45 publications

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“…3). Two of those are the transcription factors Msn2 and Msn4, which mediate the transcription of the so-called stress response element (STRE)-controlled genes (Estruch and Carlson 1993;Martinez-Pastor et al 1996;Schmitt and McEntee 1996). STRE genes are involved in a wide variety of processes, including protection against diverse types of stress such as heat, oxidative and osmotic stress, carbohydrate metabolism and growth regulation (Mai and Breeden 1997;Moskvina et al 1998;Smith et al 1998;Gasch et al 2000).…”

Section: Regulation Of the Camp-pka Pathwaymentioning

confidence: 99%

Life in the midst of scarcity: adaptations to nutrient availability in Saccharomyces cerevisiae

et al. 2010

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Cells of all living organisms contain complex signal transduction networks to ensure that a wide range of physiological properties are properly adapted to the environmental conditions. The fundamental concepts and individual building blocks of these signalling networks are generally well-conserved from yeast to man; yet, the central role that growth factors and hormones play in the regulation of signalling cascades in higher eukaryotes is executed by nutrients in yeast. Several nutrient-controlled pathways, which regulate cell growth and proliferation, metabolism and stress resistance, have been defined in yeast. These pathways are integrated into a signalling network, which ensures that yeast cells enter a quiescent, resting phase (G 0 ) to survive periods of nutrient scarceness and that they rapidly resume growth and cell proliferation when nutrient conditions become favourable again. A series of well-conserved nutrient-sensory protein kinases perform key roles in this signalling network: i.e. Snf1, PKA, Tor1 and Tor2, Sch9 and Pho85-Pho80. In this review, we provide a comprehensive overview on the current understanding of the signalling processes mediated via these kinases with a particular focus on how these individual pathways converge to signalling networks that ultimately ensure the dynamic translation of extracellular nutrient signals into appropriate physiological responses.

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Section: Regulation Of the Camp-pka Pathwaymentioning

confidence: 99%

Life in the midst of scarcity: adaptations to nutrient availability in Saccharomyces cerevisiae

et al. 2010

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“…Furthermore, several stress-responsive genes were also upregulated in surplus iron at 4 h, such as HSP12, HOR7, LSP1, SOD2, GRX4, CCC1, GSY1, and HSP31. In particular, HSP12, HOR7, SOD2, CCC1 and HSP31 contain a stress response element (STRE) consensus sequence in their promoter regions, and all are target genes of the stress-resistant transcription factors Msn2/4 (Gauci et al, 2009;Martinez-Pastor et al, 1996). For instance, Hsp12 is a plasma membrane protein induced by stress conditions that probably initially perceives extracellular surplus iron.…”

Section: Transcriptional Profiles Of Yeast In Response To Surplus Ironmentioning

confidence: 99%

“…Upon stress, Msn4 is hyperphosphorylated and relocalized to the nucleus, then displays a periodic nucleo-cytoplasmic shuttling behavior. DNA binding of Msn4 may be controlled inside the nucleus or by the regulation of nuclear entry (Martinez-Pastor et al, 1996). Therefore, we selected Msn4 fused with a green fluorescent protein (GFP) tag, designated Msn4-GFP, to monitor its dynamic distribution in surplus iron conditions.…”

Section: A B Cmentioning

confidence: 99%

Expression Profiling Reveals an Unexpected Growth-Stimulating Effect of Surplus Iron on the Yeast Saccharomyces cerevisiae

Wang

2012

Molecules and Cells

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Iron homeostasis plays a crucial role in growth and division of cells in all kingdoms of life. Although yeast iron metabolism has been extensively studied, little is known about the molecular mechanism of response to surplus iron. In this study, expression profiling of Saccharomyces cerevisiae in the presence of surplus iron revealed a dual effect at 1 and 4 h. A cluster of stress-responsive genes was upregulated via activation of the stress-resistance transcription factor Msn4, which indicated the stress effect of surplus iron on yeast metabolism. Genes involved in aerobic metabolism and several anabolic pathways are also upregulated in iron-surplus conditions, which could significantly accelerate yeast growth. This dual effect suggested that surplus iron might participate in a more complex metabolic network, in addition to serving as a stress inducer. These findings contribute to our understanding of the global response of yeast to the fluctuating availability of iron in the environment.

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“…Although Msn2 appears to have the dominant role in STRE binding, transcriptional activation experiments suggest that the roles of the two proteins overlap functionally. Consequently, full stress-dependent gene expression requires the presence of both factors and a pleiotropic stress sensitive phenotype is observed only when both MSN2 and MSN4 are inactivated (Martinez-Pastor et al 1996).…”

Section: Atf1 and Pap1 Mediate Sty1 Regulation Of Transcriptionmentioning

confidence: 99%

Stress‐activated signalling pathways in yeast

1998

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Eukaryotic cells have developed response mechanisms to combat the harmful effects of a variety of stress conditions. In the majority of cases, such responses involve changes in the gene expression pattern of the cell, leading to increased levels and activities of proteins that have stress-protective functions. Over the last few years, considerable progress has been made in understanding how stress-dependent transcriptional changes are brought about, and it transpires that the underlying mechanisms are highly conserved, being similar in organisms ranging from yeast to man. Many of the stress signals derive from the extracellular environment and accordingly these signals require transduction from the cell surface to the nucleus. This is accomplished through stress-activated signalling pathways, key amongst which are the highly conserved stress-activated MAP kinase pathways. Stimulation of these pathways leads to the increased activity of specific transcription factors and consequently the increased expression of certain stress-related genes. In this review, we focus on the progress that has been made in understanding these stress responses in yeast.

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The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE).

Cited by 979 publications

References 45 publications

Life in the midst of scarcity: adaptations to nutrient availability in Saccharomyces cerevisiae

Life in the midst of scarcity: adaptations to nutrient availability in Saccharomyces cerevisiae

Expression Profiling Reveals an Unexpected Growth-Stimulating Effect of Surplus Iron on the Yeast Saccharomyces cerevisiae

Stress‐activated signalling pathways in yeast

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