The S.pombe mei2 gene encoding a crucial molecule for commitment to meiosis is under the regulation of cAMP.

Watanabe, Yoshinori; Lino, Y.; Furuhata, Kimio; Shimoda, Chikashi; Yamamoto, Masayuki

doi:10.1002/j.1460-2075.1988.tb02873.x

Cited by 176 publications

(139 citation statements)

References 31 publications

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“…Cell numbers were monitored at regular intervals to yield the growth curve illustrated. At the indicated time points (a to e), 50to 200-ml culture samples were withdrawn and used to prepare total RNA. (B) Two separate Northern blots of these five RNA preparations were hybridized with the rasi and byri probes.…”

Section: Resultsmentioning

confidence: 99%

Schizosaccharomyces pombe ras1 and byr1 are functionally related genes of the ste family that affect starvation-induced transcription of mating-type genes.

Nadin-Davis¹,

Nasim²

1990

Mol. Cell. Biol.

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We have further investigated the function of the rasi and byri genes, which were previously shown to be critical for sexual differentiation in fission yeast cells. Several physiological similarities between strains containing null alleles of these genes supports the idea that rasi and byri are functionally closely related. Furthermore, we have found that byri is allelic to stel, one of at least 10 genes which when mutated can cause sterility. Since rasi had previously been found to be allelic to ste5, both ras and byr genes are now clearly shown to be a part of the ste gene family, thus confirming their close functional relationship. The observation that the mating-type loci could overcome the sporulation block of rasi and byri mutant strains prompted investigation of the role of the ras-byr pathway in the induction of the mating-type gene transcripts upon nitrogen starvation. By Northern analysis of RNA preparations from strains carrying wild-type or mutant rasi alleles and grown to different stages of the growth cycle, we have shown that rasi plays an important role in inducing the Pi transcript of the mating-type loci and the mei3 gene transcript. These observations provide a molecular basis for the role of the ste gene family, including rasl and byri, in meiosis and indicate that further characterization of other ste genes would be very useful for elucidating the mechanism of rasl function in fission yeast cells.

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Section: Resultsmentioning

confidence: 99%

Schizosaccharomyces pombe ras1 and byr1 are functionally related genes of the ste family that affect starvation-induced transcription of mating-type genes.

Nadin-Davis¹,

Nasim²

1990

Mol. Cell. Biol.

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“…Nutritional starvation in combination with the heterozygosity results in the inactivation of a protein kinase encoded by the pat1 gene, which triggers a transition from the mitotic cell cycle to meiosis (Beach et al 1985;Iino & Yamamoto 1985a,b;Nurse 1985;McLeod & Beach 1988;Nielsen & Egel 1990). The mei2 gene product is essential for the initiation of meiosis (Beach et al 1985;Shimoda et al 1985Shimoda et al ,1987Watanabe et al 1988), and is a critical target of Pat1 protein kinase (Watanabe et al 1997). During the mitotic cell cycle, the mei2 gene is expressed only poorly and its gene product undergoes inactivation by Pat1.…”

Section: Introductionmentioning

confidence: 99%

Microtubule‐associated coiled‐coil protein Ssm4 is involved in the meiotic development in fission yeast

1997

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“…Molecular analysis revealed that LHD2 is homologous to maize TE1 that regulates the rate of leaf initiation in corn plants [20]. TE1 displays a significant similarity to Mei2, an RNA-binding protein from Schizosaccharomyces pombe that is required for both premeiotic DNA synthesis and the first reductional division of meiosis [29]. The similarity is highest in the three regions in both protein sequences that encode RNA-recognition motifs (RRMs).…”

Section: Discussionmentioning

confidence: 99%

LEAFY HEAD2, which encodes a putative RNA-binding protein, regulates shoot development of rice

Xiong

Jiao

et al. 2006

Cell Res

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During vegetative development, higher plants continuously form new leaves in regular spatial and temporal patterns. Mutants with abnormal leaf developmental patterns not only provide a great insight into understanding the regulatory mechanism of plant architecture, but also enrich the ways to its modification by which crop yield could be improved. Here, we reported the characterization of the rice leafy-head2 (lhd2) mutant that exhibits shortened plastochron, dwarfism, reduced tiller number, and failure of phase transition from vegetative to reproductive growth. Anatomical and histological study revealed that the rapid emergence of leaves in lhd2 was resulted from the rapid initiation of leaf primordia whereas the reduced tiller number was a consequence of the suppression of the tiller bud outgrowth. The molecular and genetic analysis showed that LHD2 encodes a putative RNA binding protein with 67% similarity to maize TE1. Comparison of genome-scale expression profiles between wild-type and lhd2 plants suggested that LHD2 may regulate rice shoot development through KNOX and hormone-related genes. The similar phenotypes caused by LHD2 mutation and the conserved expression pattern of LHD2 indicated a conserved mechanism in controlling the temporal leaf initiation in grass.

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The S.pombe mei2 gene encoding a crucial molecule for commitment to meiosis is under the regulation of cAMP.

Cited by 176 publications

References 31 publications

Schizosaccharomyces pombe ras1 and byr1 are functionally related genes of the ste family that affect starvation-induced transcription of mating-type genes.

Schizosaccharomyces pombe ras1 and byr1 are functionally related genes of the ste family that affect starvation-induced transcription of mating-type genes.

Microtubule‐associated coiled‐coil protein Ssm4 is involved in the meiotic development in fission yeast

LEAFY HEAD2, which encodes a putative RNA-binding protein, regulates shoot development of rice

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