The S-locus specific glycoproteins of Brassica accumulate in the cell wall of developing stigma papillae

Kandasamy, Muthugapatti K.; Paolillo, Dominick J.; Faraday, Christopher D.; Nasrallah, June B.; Nasrallah, Mikhail E.

doi:10.1016/0012-1606(89)90119-x

Cited by 172 publications

(89 citation statements)

References 38 publications

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“…S 2 is a class II haplotype whose SRK and SLG alleles are highly diverged from SRK 6 and SLG S (Stein et al, 1991), and MAb/H8 cross-reacts with neither SLG 2 , as previously shown (Kandasamy et al, 1989), nor the S domain of SRK 2 , as demonstrated by immunological analysis of the GST-SRK 2 fusion protein ( Figure 1B). The S 2 haplotype therefore served as a negative control in these studies.…”

Section: Gstsupporting

confidence: 68%

“…Previously, we raised monoclonal antibody MAb/H8 against purified SLG 6 ( Kandasamy et al, 1989). Because the SLGlike domain of SRK 6 has 89% amino acid sequence identity with SLG 6 (Stein et al, 1991), we hypothesized that MAb/H8 would also recognize SRK 6 and possibly other class I SRK proteins as well.…”

Section: Identification Of Srk In Stigmas Of Brassicamentioning

confidence: 99%

“…1994a; Goring et al, 1993). SLG encodes an abundant glycoprotein that accumulates in the cell wall of papillar cells (Nasrallah et al, 1985b;Kandasamy et al, 1989). Based on its sequence, we had predicted SRK to encode a plasma membrane receptor protein kinase having an extracellular domain that is highly similar to SLG, a single pass transmembrane domain, and a cytoplasmic kinase domain (Stein, et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

“…Greatest amino acid divergence, up to 33%, is observed between alleles derived from class I and class II haplotypes (Chen and Nasrallah, 1990;Stein et al, 1991), and this divergence has allowed the development of class I-specific antibodies to SLG (Kandasamy et al, 1989). Of great interest is the finding that despite their extensive divergence between haplotypes, SLG and the SLG-like domain of SRK share a high degree of sequence identity within a given haplotype, showing as little as 10% divergence (Stein et al, 1991;Goring and Rothstein, 1992;Delorme et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

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SRK, the stigma-specific S locus receptor kinase of Brassica, is targeted to the plasma membrane in transgenic tobacco.

et al. 1996

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The S locus receptor kinase (SRK) gene is one of two S locus genes required for the self-incompatibility response in Brassica. We have identified the product of the SRKs gene in 6. oleracea stigmas and have shown that it has characteristics of an integral membrane protein. When expressed in transgenic tobacco, SRK6 is glycosylated and targeted to the plasma membrane. These results provide definitive biochemical evidence for the existence in plants of a plasma membrane-localized transmembrane protein kinase with a known cell-cell recognition function. The timing of SRK expression in stigmas follows a time course similar to that previously described for another S locus-linked gene, the S locus glycoprotein (SLG) gene, and correlates with the ability of stigmas to mount a self-incompatibility response. Based on SRK6 promoter studies, the site of gene expression overlaps with that of SLG and exhibits predominant expression in the stigmatic papillar cells. Although reporter gene studies indicated that the SRK promoter was active in pollen, SRK protein was not detected in pollen, suggesting that SRK functions as a cell surface receptor exclusively in the papillar cells of the stigma.

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Section: Gstsupporting

confidence: 68%

Section: Identification Of Srk In Stigmas Of Brassicamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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SRK, the stigma-specific S locus receptor kinase of Brassica, is targeted to the plasma membrane in transgenic tobacco.

et al. 1996

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“…Because I. trifi da has the same genetic SI system as Brassica species, it might be expected that products of genes homologous to SLG or SRK would be present in the reproductive tissues of I. trifi da. SRK is known as a female S -gene (Stein et al 1991 ;Takasaki et al 2000 ), and SLG is an S -gene-related gene in Brassica (Kandasamy et al 1989 ). Ipomoea secreted glycoprotein genes ( ISG1 , -2 , and -3 ) were isolated from a mature stigma cDNA library, and the sequences of these genes showed structural similarities to Brassica SLGs.…”

Section: Several Approaches To Identifying S -Genes In I Trifi Damentioning

confidence: 99%

Self-Incompatibility System of Ipomoea trifida, a Wild-Type Sweet Potato

Tsuchiya

2014

Sexual Reproduction in Animals and Plants

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Diploid Ipomoea trifi da (Convolvulaceae) is a close relative of the cultivated hexaploid Ipomoea batatas , the cultivated sweet potato. These plants have sporophytic self-incompatibility that is regulated by a single multiallelic locus, designated as the S -locus. Genetic analyses of I. trifi da plants collected from Central America identifi ed about 50 different S -haplotypes with a linear dominance hierarchy having some codominance relationships. A linkage map of DNA markers around the S -locus indicated that the S -locus is delimited to 0.23 cM. Within the S -locus genomic region, a hypervariable genomic region of 35-95 kbp was identifi ed, and we designated this region SDR ( S -locus-specifi c divergent region). Of the several genes located within the SDR, one anther-specifi c gene, AB2 , and three stigma-specifi c genes, SE1 , SE2 , and SEA , are candidate S -genes that may encode male and female S -determinants of self-incompatibility.Keywords Ipomoea trifi da • Self-incompatibility • Sweet potato IntroductionSelf-incompatibility (SI) is a genetic mechanism to prevent self-fertilization (and thus encourage outcrossing) in angiosperms. In self-incompatible plants, when a pollen grain is recognized as the same type as self, some stage of pollen germination, pollen tube elongation, ovule fertilization, or embryo development is halted, and no seeds are produced. SI is classifi ed into several groups: homomorphic SI, heteromorphic SI, cryptic SI (CSI), and late-acting SI. Heteromorphic SI is classifi ed into two groups: distyly is determined by a single locus, which has two alleles, and tristyly is determined by two loci, each with two alleles. Heteromorphic SI is sporophytic, in that both alleles in the male plant determine the SI response in the pollen. CSI exists in a limited number of taxa (for example, there is evidence for CSI in Silene vulgaris in the Caryophyllaceae; Glaettli 2004 ). In this mechanism, the simultaneous presence of cross-and self-pollen on the same stigma results in higher seed set from cross-pollen (Bateman 1956 ). Late-acting SI is also termed ovarian SI. In this mechanism, self-pollen germinates and reaches the ovules, but no fruit is set (Seavey and Bawa 1986 ;Sage et al. 1994 ).Homomorphic SI is classifi ed into sporophytic SI (SSI) and gametophytic SI (GSI) . The SSI system is found in species of several plant families, such as the Brassicaceae, Asteraceae, Malvaceae, Betulaceae, Sterculiaceae, Polemoniaceae, and Convolvulaceae (de Nettancourt 2001 ; Allen and Hiscock 2008 ). In the plants in these families, SI is genetically regulated by a single multi-allelic locus, the S -locus . Within the S -locus, a pair of genes (named S -genes ), one encoding the male-determinant molecules and the other the female-determinant molecules, is localized. These genes are essential for the SI reaction, because these gene products contribute to self/non-self recognition. The sets of S -genes are tightly linked at the S -locus, and the S -locus (also called S -haplotype : Nasrallah and Na...

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Peptides as Signals

Xia

2018

Annual Plant Reviews Online

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The S-locus specific glycoproteins of Brassica accumulate in the cell wall of developing stigma papillae

Cited by 172 publications

References 38 publications

SRK, the stigma-specific S locus receptor kinase of Brassica, is targeted to the plasma membrane in transgenic tobacco.

SRK, the stigma-specific S locus receptor kinase of Brassica, is targeted to the plasma membrane in transgenic tobacco.

Self-Incompatibility System of Ipomoea trifida, a Wild-Type Sweet Potato

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