The RPA190-pc gene participates in the regulation of metalaxyl sensitivity, pathogenicity and growth in Phytophthora capsici

Wang, Weiyan; Liu, Dong; Xin, Zhuo; Wang, Yiye; Song, Zhiqiang; Chen, Fangxin; Pan, Yuemin; Gao, Zhimou

doi:10.1016/j.gene.2020.145081

Cited by 10 publications

(10 citation statements)

References 27 publications

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“…Previous studies have revealed that metalaxyl inhibits the synthesis of ribosomal RNA, suggesting that it may target RNA polymerase I in P. infestans ( Chen et al., 2018 ). To date, several studies has shown an assay based on RPA190 will not be sufficient to mediate the sensitivity levels of Phytophthora resistance isolates ( Matson et al., 2015 ; Wang et al., 2021 ) and other genes may contribute to stable metalaxyl resistance. In this study, we observed that the mutants of four PsRLK genes (ΔPsRLK12/16/20/23) increased sensitivity to metalaxyl but not fluopicolide ( Figure S4 ).…”

Section: Discussionmentioning

confidence: 99%

Phytophthora sojae leucine-rich repeat receptor-like kinases: diverse and essential roles in development and pathogenicity

Pei

Shen

et al. 2021

iScience

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Leucine-rich repeat receptor-like kinases (LRR-RLKs) are critical signal receptors in plant development and defense. Like plants, oomycete pathogen genomes also harbor LRR-RLKs, but their functions remain largely unknown. Here, we systematically characterize all the 24 LRR-RLK genes (PsRLKs) from Phytophthora sojae, which is a model of oomycete pathogens. Although none of them was required for vegetative growth, the specific PsRLKs are important for stress responses, zoospore production, zoospores chemotaxis, and pathogenicity. Interestingly, the Ga subunit PsGPA1 interacts with the five chemotaxis-related PsRLKs via their intracellular kinase domains, and expression of PsGPA1 gene is downregulated in the three mutants (DPsRLK17/22/24). Moreover, we generated the PsRLK-PsRLK interaction network of P. sojae and found that PsRLK21, together with PsRLK10 or PsRLK17, regulate virulence by direct association. Taken together, our results reveal the diverse roles of LRR-RLKs in modulating P. sojae development, interaction with soybean, and responses to diverse environmental factors.

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Section: Discussionmentioning

confidence: 99%

Phytophthora sojae leucine-rich repeat receptor-like kinases: diverse and essential roles in development and pathogenicity

Pei

Shen

et al. 2021

iScience

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“…Similar conclusions were also reached by Chen et al (2018). It was also considered that the analogical gene RPA190-pc was associated with the development of oospores and mycelial growth, and that mutations on this gene also have an impact on changes in the pathogenicity (Wang et al 2021). Multiple point mutations were revealed in the locus of this gene; a total of 35 SNPs (single nucleotide polymorphisms) were found in the RPA190 locus in P. infestans, whose total length is 5 433 bp (Chen et al 2018).…”

Section: Genetic Background Of Resistance To Metalaxyl In Some Soil P...mentioning

confidence: 63%

“…They concluded that genes other than RPA190 are involved in the resistance, and suggested the RPA135 gene as an addition. Using an analysis of up-and down-regulated genes, Wang et al (2021) recently demonstrated that the RPA190 gene is the key to resistance for both the in vitro and in vivo growth of P. capsici in the presence of metalaxyl. This gene, which encodes a large subunit of the RNA polymerase I, seems to be under positive selection.…”

Section: Genetic Background Of Resistance To Metalaxyl In Some Soil P...mentioning

confidence: 99%

“…Although the revelation of this single gene conforms to the Mendelian mode of heritability, some other genes are also considered as possibly involved in the metalaxyl resistance (Montes et al 2016;Chen et al 2018). As follows, metalaxyl is a site-specific inhibitor having only a single target in the metabolism of the species of Peronosporomycetes, which is considered a cause of the quick formation of resistance in members of this group against this compound (Davidse et al 1983;Gisi & Sierotzki 2008;Hu & Li 2014;Wang et al 2021). However, except for the main target -RNA polymerase I, in the metabolic profiling of P. infestans, another 49 metabolites were differently expressed during cultivation of resistant isolates in the presence of metalaxyl in comparison to sensitive ones (Maridueña-Zavala et al 2017).…”

Section: Genetic Background Of Resistance To Metalaxyl In Some Soil P...mentioning

confidence: 99%

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Use of metalaxyl against some soil plant pathogens of the class Peronosporomycetes - A review and two case studies

Pánek¹,

Helmer²,

Ali³

2022

Plant Prot. Sci.

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Upon its discovery and implementation in plant protection, metalaxyl became one of the most important fungicides against Phytophthora infestans, but its efficiency has also been proven against other soil pathogens of the class Peronosporomycetes. The most important genus – Phytophthora comprises more than 150 plant pathogens, which cause significant losses in crop production or damage to natural plant associations. Many species of related genera Pythium, Phytopythium and Globisporangium have a similar ability as the species of Phytophthora. Those pathogens are able to quickly spread in wet soils by actively movable zoospores or in the air by means of zoosporangia; they are able to persist in an environment for long periods once they are introduced into the locality, having durability from their resting structures (chlamydospores, hyphal swelling and oospores). Metalaxyl has proven to be very efficient against these pathogens. However, shortly after its release, the rapid development of resistance against this compound was recorded in many species of the class Peronosporomycetes. Such easily developed resistance is due to the monogenic nature of the resistance, which also determines any anti-resistant strategies. The solution of this issue rests in the cautious use of metalaxyl, with consideration given to these strategies, and should be based also on precise information about the environment and the present pathogenic agents.

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“…Despite the success achieved over the years with the use of chemical control, the inappropriate use of molecules has made some P. capsici isolates insensitive to commercial oomyceticides such as metalaxyl and mefenoxam [77,78]. A solution to reduce these effects on the oomycete is the use of active ingredients such as mandipropamide and dimethomorph, considered molecules with low to medium risk of resistance [79,80].…”

Section: Chemical Controlmentioning

confidence: 99%

Phytophthora capsici on Capsicum Plants: A Destructive Pathogen in Chili and Pepper Crops

Moreira-Morrillo¹,

Monteros-Altamirano²,

Reis³

et al. 2023

Capsicum - Current Trends and Perspectives

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Capsicum from tropical and subtropical America, is an important genus for the nutritional, economic and cultural values of its species. At the same time, the Capsicum species are affected by diseases caused by viruses, bacteria, fungi and pseudofungi, in particular the oomycete Phytophthora capsici. This phytopathogen causes great damage and losses in different Capsicum species, because it infects all plant organs causing root, crown and fruit rot; and, leaf blight. The polycyclic dispersion through zoospores and sporangia, the limited availability of resistant genotypes, and the reduced diversity of effective oomyceticides (fungicides), make P. capsici one of the most complex phytopathogens to be managed worldwide specially in field conditions. However, successful management of P. capsici depends on the knowledge of the pathogen, its interaction with the susceptible host and the methods of control used. Thus, this chapter addressed the etiology, symptomatology, occurrence and management of the disease. Additionally, the cycle of the disease is discussed in a holistic and simple way.

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The RPA190-pc gene participates in the regulation of metalaxyl sensitivity, pathogenicity and growth in Phytophthora capsici

Cited by 10 publications

References 27 publications

Phytophthora sojae leucine-rich repeat receptor-like kinases: diverse and essential roles in development and pathogenicity

Phytophthora sojae leucine-rich repeat receptor-like kinases: diverse and essential roles in development and pathogenicity

Use of metalaxyl against some soil plant pathogens of the class Peronosporomycetes - A review and two case studies

Phytophthora capsici on Capsicum Plants: A Destructive Pathogen in Chili and Pepper Crops

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