The Royan Public Umbilical Cord Blood Bank: Does It Cover All Ethnic Groups in Iran Based on HLA Diversity?

Ebrahimkhani, Saeideh; Farjadian, Shirin; Ebrahimi, Marzieh

doi:10.1159/000357997

Cited by 2 publications

(3 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Designing a universal gRNAs for HLA-A alleles Since HLA genes are among the most polymorphic region of human genome, finding a consensus region among various alleles of an HLA gene was extremely difficult. We collected all HLA-A allele sequences of ethnic groups, which cover >99.9% of the Iranian population according to the Royan Public Umbilical Cord Blood Bank registry [14]. The coding sequences which were elicited from IPD-IMGT/HLA [15] were aligned simultaneously, and the appropriated gRNAs were picked out among the consensus regions.…”

Section: Resultsmentioning

confidence: 99%

“…HLA-A and HLA-B were our candidates, which are more reliable to be knockout. However, designing a unique gRNA for HLA-B is almost impossible because of massive allelic diversity in populations [14,29]. The main reason for not choosing HLA-C as a target gene was its inhibitory effects on NK cell receptors [30].…”

Section: Discussionmentioning

confidence: 99%

“…We hired CRISPR/Cas9 system to generate HLA-A null cells. With considering the frequency of Iranian HLA alleles [14], we applied dual gRNAs (A-gRNA1 and A-gRNA3) among few conserved sequences to make a large deletion (Fig. 3) as a straightforward and costbenefit method rather than single gRNA/Cas9 [36].…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

HLA-A gene knockout using CRISPR/Cas9 system toward overcoming transplantation concerns

Amiri

Ranjbar

Pirouzfar

et al. 2021

Egypt J Med Hum Genet

View full text Add to dashboard Cite

Background The treatment of many cancers and genetic diseases relies on novel engraftment approaches such as cell therapy and hematopoietic stem cell transplantation (HSCT). However, these methods are hindered by the alloreactive immune responses triggered by incompatible human leukocyte antigen (HLA) molecules. A successful HSCT procedure requires the eradication of donor and recipient HLA alloimmunization. Eliminating HLA-A gene expression using clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 nuclease (CRISPR/Cas9) could be a great approach to increase the possibility of a successful HSCT through extending pool of unrelated donors. Results Our dual gRNA approach introduced a large deletion in the HLA-A gene. Among 22 single-cloned cells, two clones (9.09%) and 11 clones (50%) received homozygous and heterozygous large deletions, respectively. Finally, the real-time PCR results also revealed that HLA-A gene expression was diminished significantly. Conclusion The results suggested that CRISPR/Cas9 could be used as an efficient technique to introduce HLA-A gene knockout; thus, it can considerably lessen the burden of finding a fully matched donor by lowering the alleles required for a successful HSCT.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

HLA-A gene knockout using CRISPR/Cas9 system toward overcoming transplantation concerns

Amiri

Ranjbar

Pirouzfar

et al. 2021

Egypt J Med Hum Genet

View full text Add to dashboard Cite

show abstract

Immunoinformatics Evaluation of a Fusion Protein Composed of Leishmania infantum LiHyV and Phlebotomus kandelakii Apyrase as a Vaccine Candidate against Visceral Leishmaniasis

Fayaz¹,

Bahrami²,

Fard-Esfahani³

et al. 2022

IJPA

View full text Add to dashboard Cite

Background: Visceral leishmaniasis (VL) is a lethal parasitic disease, transmitted by sand fly vectors. Immunomodulatory properties of sand fly saliva proteins and their protective effects against Leishmania infection in pre-exposed animals suggest that a combination of an antigenic salivary protein along with a Leishmania antigen can be considered for designing a vaccine against leishmaniasis Methods: Three different fusion forms of L. infantum hypothetical protein (LiHyV) in combination with Phlebotomus kandelakii salivary apyrase (PkanAp) were subjected to in-silico analyses. Major Histocompatibility Complex (MHC) class I and II epitopes in both humans and BALB/c mice were predicted. Antigenicity, immunogenicity, epitope conservancy, toxicity, and population coverage were also evaluated. Results: Highly antigenic promiscuous epitopes consisting of truncated LiHyV (10-285) and full-length PkanAp (21-329) were identified in human and was named Model 1. This model contained 25 MHC-I and 141 MHC-II antigenic peptides which among them, MPANSDIRI and AQSLFDFSGLALDSN were fully conserved. LALDSNATV, RCSSALVSI, ALVSINVPL, SAVESGALF of MHC-I epitopes, and 28 MHC-II binding epitopes showed 60% conservancy among various clades. A population coverage with a rate of >75% in the Iranian population and >70% in the whole world was also identified. Conclusion: Based on this in-silico approach, the predicted Model 1 could potentially be used as a vaccine candidate against VL.

show abstract

The Royan Public Umbilical Cord Blood Bank: Does It Cover All Ethnic Groups in Iran Based on HLA Diversity?

Cited by 2 publications

References 27 publications

HLA-A gene knockout using CRISPR/Cas9 system toward overcoming transplantation concerns

HLA-A gene knockout using CRISPR/Cas9 system toward overcoming transplantation concerns

Immunoinformatics Evaluation of a Fusion Protein Composed of Leishmania infantum LiHyV and Phlebotomus kandelakii Apyrase as a Vaccine Candidate against Visceral Leishmaniasis

Contact Info

Product

Resources

About