The Roseibium album (Labrenzia alba) Genome Possesses Multiple Symbiosis Factors Possibly Underpinning Host-Microbe Relationships in the Marine Benthos

Couceiro, Joana Fernandes; Keller‐Costa, Tina; Marques, Matilde; Kyrpides, Nikos C.; Woyke, Tanja; Whitman, William B.; Costa, Rodrigo

doi:10.1128/mra.00320-21

Cited by 6 publications

(5 citation statements)

References 14 publications

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“…The “MicroEcoEvo” collection harbored as many as 62 Roseibium strains and, in this study, Roseibium album strains Alg231_36 and EL143 showed inhibitory activity towards human-pathogenic bacteria and C. glabrata. In line with earlier findings for the genus [ 67 ], both strains harbored transAT-PKS, T3PKS, NRPS and multiple RiPP-like SM-BGCs, underpinning their potential to produce a diverse range of secondary metabolites.…”

Section: Discussionsupporting

confidence: 88%

Marine Sponge and Octocoral-Associated Bacteria Show Versatile Secondary Metabolite Biosynthesis Potential and Antimicrobial Activities against Human Pathogens

et al. 2022

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Marine microbiomes are prolific sources of bioactive natural products of potential pharmaceutical value. This study inspected two culture collections comprising 919 host-associated marine bacteria belonging to 55 genera and several thus-far unclassified lineages to identify isolates with potentially rich secondary metabolism and antimicrobial activities. Seventy representative isolates had their genomes mined for secondary metabolite biosynthetic gene clusters (SM-BGCs) and were screened for antimicrobial activities against four pathogenic bacteria and five pathogenic Candida strains. In total, 466 SM-BGCs were identified, with antimicrobial peptide- and polyketide synthase-related SM-BGCs being frequently detected. Only 38 SM-BGCs had similarities greater than 70% to SM-BGCs encoding known compounds, highlighting the potential biosynthetic novelty encoded by these genomes. Cross-streak assays showed that 33 of the 70 genome-sequenced isolates were active against at least one Candida species, while 44 isolates showed activity against at least one bacterial pathogen. Taxon-specific differences in antimicrobial activity among isolates suggested distinct molecules involved in antagonism against bacterial versus Candida pathogens. The here reported culture collections and genome-sequenced isolates constitute a valuable resource of understudied marine bacteria displaying antimicrobial activities and potential for the biosynthesis of novel secondary metabolites, holding promise for a future sustainable production of marine drug leads.

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Section: Discussionsupporting

confidence: 88%

Marine Sponge and Octocoral-Associated Bacteria Show Versatile Secondary Metabolite Biosynthesis Potential and Antimicrobial Activities against Human Pathogens

et al. 2022

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“…Similarly, we found in generation three that OTU 2602 (Labrenzia alba) was absent in virgin PBAT and weathered LDPE compared to control. Although this OTU was only present in low read numbers, it is possible that MP impaired the symbiotic relationship between copepods and Labrenzia alba, as it was shown that the genome of this species possesses symbiosis factors between microbes and marine invertebrate hosts (Couceiro et al, 2021). However, even if the Wilcoxon test revealed several significant OTUs impacted by MP exposure, since these OTUs were only supported by low read numbers, we suggest considering these findings with caution.…”

Section: Main Effectsmentioning

confidence: 75%

Multigenerational exposure of microplastics on the microbiota of E. affinis (copepod): a comparative study between biodegradable and nonbiodegradable microplastics

Thery,

Li,

Das

et al. 2023

Front. Ecol. Evol.

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The accumulation of plastic debris around the world, especially in marine environments, has been well documented during the past decades. Recent studies have found that inorganic surfaces of microplastics (MPs) can be used by microorganisms as living substrates and form an ecosystem named “plastisphere.” Some microorganisms present in MPs are capable of producing polymer-degrading enzymes. In addition, MPs can also serve as vectors and carry microorganisms (including potential pathogens) into higher trophic levels through their ingestion by animals. In this study, impacts on copepod microbiota during chronic exposure to MPs were investigated by exposing copepods to a classic single-use polymer (low-density polyethylene (LDPE)) and a biodegradable polymer (polybutylene adipate terephthalate (PBAT)). Copepods were exposed to “virgin” and “weathered” MPs during four generations at an environmentally relevant concentration of 300 µg/L, followed by one “detoxification” generation without MP exposition. Impacts of MP exposure on copepod microbiota were investigated using 16S rRNA gene high-throughput sequencing. The result of nonmetric multidimensional scaling (NMDS) analysis showed that copepods (with or without MP exposure) carried distinguishable microbiota as compared with the microbiota of water and microalgae used for maintaining copepods. According to the results of permutational analysis of variance (PERMANOVA), the microbiota of MP-exposed (both PBAT and LDPE) copepods was significantly different from the microbiota of unexposed copepods during generations one to four. After “detoxification,” however, no significant difference in microbiota composition was observed among all generation five copepods. Altogether, impacts on copepod microbiota of MP exposure for multiple generations were observed, despite plastic origin (biodegradable or not) and aging conditions. Furthermore, copepod microbiota seemed to return to their original structure as soon as the MP exposure stopped.

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“…Alphaproteobacterial Stappiaceae are related to the abundant Roseobacteraceae . The genera include bacteriochlorophyll-producing Roseibium , members of which have been isolated from red algae [ 88 ], corals [ 89 ], oysters [ 90 ], and dinoflagellates [ 91 ], possibly also in a symbiotic function. Nitrincolaceae (formerly Oceanospirillaceae ), abundant in PA3 communities, are opportunistic Gammaproteobacteria that frequently associate with phytoplankton (e.g., [ 92 ]), including Reinekea species, which we have observed in high abundance at Helgoland Roads before [ 42 , 93 ].…”

Section: Discussionmentioning

confidence: 99%

Particle-attached bacteria act as gatekeepers in the decomposition of complex phytoplankton polysaccharides

Wang,

Bartosik,

Sidhu

et al. 2024

Microbiome

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Background Marine microalgae (phytoplankton) mediate almost half of the worldwide photosynthetic carbon dioxide fixation and therefore play a pivotal role in global carbon cycling, most prominently during massive phytoplankton blooms. Phytoplankton biomass consists of considerable proportions of polysaccharides, substantial parts of which are rapidly remineralized by heterotrophic bacteria. We analyzed the diversity, activity, and functional potential of such polysaccharide-degrading bacteria in different size fractions during a diverse spring phytoplankton bloom at Helgoland Roads (southern North Sea) at high temporal resolution using microscopic, physicochemical, biodiversity, metagenome, and metaproteome analyses. Results Prominent active 0.2–3 µm free-living clades comprised Aurantivirga, “Formosa”, Cd. Prosiliicoccus, NS4, NS5, Amylibacter, Planktomarina, SAR11 Ia, SAR92, and SAR86, whereas BD1-7, Stappiaceae, Nitrincolaceae, Methylophagaceae, Sulfitobacter, NS9, Polaribacter, Lentimonas, CL500-3, Algibacter, and Glaciecola dominated 3–10 µm and > 10 µm particles. Particle-attached bacteria were more diverse and exhibited more dynamic adaptive shifts over time in terms of taxonomic composition and repertoires of encoded polysaccharide-targeting enzymes. In total, 305 species-level metagenome-assembled genomes were obtained, including 152 particle-attached bacteria, 100 of which were novel for the sampling site with 76 representing new species. Compared to free-living bacteria, they featured on average larger metagenome-assembled genomes with higher proportions of polysaccharide utilization loci. The latter were predicted to target a broader spectrum of polysaccharide substrates, ranging from readily soluble, simple structured storage polysaccharides (e.g., laminarin, α-glucans) to less soluble, complex structural, or secreted polysaccharides (e.g., xylans, cellulose, pectins). In particular, the potential to target poorly soluble or complex polysaccharides was more widespread among abundant and active particle-attached bacteria. Conclusions Particle-attached bacteria represented only 1% of all bloom-associated bacteria, yet our data suggest that many abundant active clades played a pivotal gatekeeping role in the solubilization and subsequent degradation of numerous important classes of algal glycans. The high diversity of polysaccharide niches among the most active particle-attached clades therefore is a determining factor for the proportion of algal polysaccharides that can be rapidly remineralized during generally short-lived phytoplankton bloom events.

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The Roseibium album (Labrenzia alba) Genome Possesses Multiple Symbiosis Factors Possibly Underpinning Host-Microbe Relationships in the Marine Benthos

Cited by 6 publications

References 14 publications

Marine Sponge and Octocoral-Associated Bacteria Show Versatile Secondary Metabolite Biosynthesis Potential and Antimicrobial Activities against Human Pathogens

Marine Sponge and Octocoral-Associated Bacteria Show Versatile Secondary Metabolite Biosynthesis Potential and Antimicrobial Activities against Human Pathogens

Multigenerational exposure of microplastics on the microbiota of E. affinis (copepod): a comparative study between biodegradable and nonbiodegradable microplastics

Particle-attached bacteria act as gatekeepers in the decomposition of complex phytoplankton polysaccharides

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