The Rose Bengal Test for Liver Function

Topical rose Bengal: Pre‐clinical evaluation of pharmacokinetics and safety

“…Since the fluorimetric assay can detect RB whenever this 60 ml of plasma contains >1.2 mg RB, the lack of measurable plasma levels upon application of 1% RB to an 80 cm 2 patch of skin makes it clear that less than 0.002% of the applied RB (assuming 6 ml of 1% RB, comprising a total applied dose of 60 mg RB) is present in plasma at any time point. Moreover, it is likely that the high efficiency of hepatic excretion of RB [9][10][11][12][13][14][15] assures that any RB that might cross Diamonds, control skin treated with saline only; squares, circles, and triangles represent skin treated with RB in saline at 0.0001, 0.001, and 0.01% w/v, respectively. Each data point comprises an average response (N ¼ 5) for all rabbits illuminated under the respective conditions; maximum standard deviation from average response at any time point was 1.28C.…”

“…RB has an intersystem crossing quantum yield approaching unity (F isc ¼ 0.98) and a high singlet oxygen yield (r( 1 O 2 *) > 0.75), indicating that RB is capable of highly efficient 1 O 2 * production upon irradiation with green light [8]. Its long history of safe use as a systemic diagnostic of hepatic function [9][10][11][12][13][14][15][16][17][18][19][20][21] as well as a topical ophthalmic diagnostic [22][23][24][25][26][27][28] suggests that, in marked contrast to many photodynamic therapy (PDT) agents, RB should have minimal potential for side effects, such as prolonged photosensitivity.…”

Advances in the Development of Intralesional Therapies for Melanoma

“…PV-10 is a sterile, nonpyrogenic solution derived from Rose Bengal disodium, a reddark xanthene-based dye developed for use in liver and ophthalmological diagnostic studies [32,33]. Mouse models have shown that PV-10 has direct cytotoxic effects on cancer cells, by lysozyme-induced apoptosis and necrosis [34,35].…”

Liver function in congestive heart failure

“…Serby and Bloch (19) studied nine patients with heart disease, five of whom had an enlarged liver, but in only one was there a retention of bromsulphalein. Epstein, Delprat and Kerr (20) studied the elimination of rose bengal in seven cases of congestive heart failure. All were normal except one with marked anasarca.…”