The Roles of the LIM Domain Proteins in Drosophila Cardiac and Hematopoietic Morphogenesis

She, Meihua; Tang, Min; Jiang, Tingting; Zeng, Qun

doi:10.3389/fcvm.2021.616851

Cited by 7 publications

(5 citation statements)

References 59 publications

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“…The definitive identification of hub nuclei allowed analysis for upregulated genes (log 2 FC ≥1, compared to the full testis plus seminal vesicle dataset; Figure 7—source data 1 ) as new candidate hub markers. One such encodes the transcription factor Tailup (Tup, also known as Islet Thor and Thomas, 1997 ; She et al, 2021 ; Boukhatmi et al, 2012 ; Figure 7E ). Indeed, a Tup::GFP transgene showed strong protein expression marking hub nuclei at the apex of adult testes ( Figure 7F–F’’’ ).…”

Section: Resultsmentioning

confidence: 99%

Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq of Drosophila testes

Raz

Vida²,

Stern

et al. 2023

eLife

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Proper differentiation of sperm from germline stem cells, essential for production of the next generation, requires dramatic changes in gene expression that drive remodeling of almost all cellular components, from chromatin to organelles to cell shape itself. Here, we provide a single nucleus and single cell RNA-seq resource covering all of spermatogenesis in Drosophila starting from in-depth analysis of adult testis single nucleus RNA-seq (snRNA-seq) data from the Fly Cell Atlas (FCA) study. With over 44,000 nuclei and 6000 cells analyzed, the data provide identification of rare cell types, mapping of intermediate steps in differentiation, and the potential to identify new factors impacting fertility or controlling differentiation of germline and supporting somatic cells. We justify assignment of key germline and somatic cell types using combinations of known markers, in situ hybridization, and analysis of extant protein traps. Comparison of single cell and single nucleus datasets proved particularly revealing of dynamic developmental transitions in germline differentiation. To complement the web-based portals for data analysis hosted by the FCA, we provide datasets compatible with commonly used software such as Seurat and Monocle. The foundation provided here will enable communities studying spermatogenesis to interrogate the datasets to identify candidate genes to test for function in vivo.

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Section: Resultsmentioning

confidence: 99%

Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq of Drosophila testes

Raz

Vida²,

Stern

et al. 2023

eLife

View full text Add to dashboard Cite

show abstract

“…The definitive identification of hub nuclei allowed analysis for upregulated genes (log 2 FC ≥ 1, compared to the full testis plus seminal vesicle dataset; Figure 7 - source data 1) as new candidate hub markers. One such gene encodes the transcription factor Tailup (Tup, also known as Islet (Boukhatmi et al, 2012; She et al, 2021; Thor and Thomas, 1997) (Figure 7E). Indeed, a Tup::GFP transgene showed strong protein expression marking hub nuclei at the apex of adult testes (Figure 7F-F’’’).…”

Section: Resultsmentioning

confidence: 99%

“…The definitive identification of hub nuclei allowed analysis for upregulated genes (log 2 FC ≥ 1, compared to the full testis plus seminal vesicle dataset; Figure 7 -source data 1) as new candidate hub markers. One such encodes the transcription factor Tailup (Tup, also known as Islet (Thor and Thomas 1997;She et al, 2021;Boukhatmi et al, 2012) (Figure 7E). Indeed, a…”

Section: The Hub: Architectural Organizer and Key Signaling Centermentioning

confidence: 99%

Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq ofDrosophilatestes

Raz

Vida²,

Stern

et al. 2022

Preprint

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Proper differentiation of sperm from germline stem cells, essential for production of the next generation, requires dramatic changes in gene expression that drive remodeling of almost all cellular components, from chromatin to organelles to cell shape itself. Here we provide a single nucleus and single cell RNA-seq resource covering all of spermatogenesis in Drosophila starting from in-depth analysis of adult testis single nucleus RNA-seq (snRNA-seq) data from the Fly Cell Atlas (FCA) study (Li et al., 2022). With over 44,000 nuclei and 6,000 cells analyzed, the data provide identification of rare cell types, mapping of intermediate steps in differentiation, and the potential to identify new factors impacting fertility or controlling differentiation of germline and supporting somatic cells. We justify assignment of key germline and somatic cell types using combinations of known markers, in situ hybridization, and analysis of extant protein traps. Comparison of single cell and single nucleus datasets proved particularly revealing of dynamic developmental transitions in germline differentiation. To complement the web-based portals for data analysis hosted by the FCA, we provide datasets compatible with commonly used software such as Seurat and Monocle. The foundation provided here will enable communities studying spermatogenesis to interrogate the datasets to identify candidate genes to test for function in vivo.

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“…The LMO7 protein is predominantly expressed in cardiac and skeletal muscle tissues ( 13 ). LMO7 protein encompasses multiple LIM domains, serving as protein-protein interaction motifs implicated in cell growth, differentiation and cytoskeletal organization ( 14 – 16 ). In addition, LMO7 contains PDZ domains, facilitating interaction modules that typically bind to specific protein sequences, and which are involved in protein localization, signal transduction and the assembly of protein complexes within cells ( 17 , 18 ).…”

Section: Molecular Characteristics and Biological Functions Of Lmo7mentioning

confidence: 99%

Role of LMO7 in cancer (Review)

Zeng,

Jiang,

Wang

2024

Oncol Rep

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Cancer constitutes a multifaceted ailment characterized by the dysregulation of numerous genes and pathways. Among these, LIM domain only 7 (LMO7) has emerged as a significant player in various cancer types, garnering substantial attention for its involvement in tumorigenesis and cancer progression. This review endeavors to furnish a comprehensive discourse on the functional intricacies and mechanisms of LMO7 in cancer, with a particular emphasis on its potential as both a therapeutic target and prognostic indicator. It delves into the molecular attributes of LMO7, its implications in cancer etiology and the underlying mechanisms propelling its oncogenic properties. Furthermore, it underscores the extant challenges and forthcoming prospects in targeting LMO7 for combating cancer.

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The Roles of the LIM Domain Proteins in Drosophila Cardiac and Hematopoietic Morphogenesis

Cited by 7 publications

References 59 publications

Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq of Drosophila testes

Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq of Drosophila testes

Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq ofDrosophilatestes

Role of LMO7 in cancer (Review)

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