The roles of ribosomal protein S19 C-terminus in a shortened neutrophil lifespan through delta lactoferrin

Nishiura, Hiroshi; Yamanegi, Koji; Kawabe, Mayumi; Kato-Kogoe, Nahoko; Yamada, Naoko; Nakasho, Keiji

doi:10.1016/j.imbio.2015.05.006

Cited by 7 publications

(15 citation statements)

References 42 publications

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“…We found that 10 −8 M S‐tagged C5a attracted similar numbers of the macrophage‐like or the neutrophil‐like HL‐60 mock cells (Fig. a) . A three‐fold increase in the chemotactic potency of neutrophil‐like HL‐60 mock cells against S‐tagged C5a indicated that the expression of C5aR on neutrophil‐like cells was higher than that on macrophage‐like cells (Fig.…”

Section: Resultsmentioning

confidence: 75%

“…Moreover, the chemotactic potency of the macrophage‐like HL‐60 mock cells towards S‐tagged C5a was higher than that towards S‐tagged C5a/RP S19. Our data suggest that low concentrations of neutrophil‐specific δLf and/or δANXA3 are present in the macrophage‐like HL‐60 mock cells …”

Section: Resultsmentioning

confidence: 75%

“…To examine the effects of δLf and δANXA3 co‐expression on C5aR‐mediated responses, we prepared HL‐60 cells containing empty vector or vector with δANXA3 and δLf cDNA (HL‐60 mock cells or HL‐60 δLf/δANXA3 cells). We had previously generated HL‐60 δLf cells and HL‐60 δANXA3 cells . The expression ratios of δLf vs full‐length Lf in HL‐60 δLf cells or δANXA3 vs full‐length ANXA3 in HL‐60 δANXA3 cells were studied by Western blotting with anti‐Lf or anti‐ANXA3 rabbit IgG, respectively (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…To generate HL‐60 transformed cells containing pCAGGS‐CSII‐EF‐δANXA3 cDNA, a solution of plasmid DNA containing packaging plasmid and plasmids expressing VSV‐G and Rev was mixed with 293T cells . After ultracentrifugation, the HIV‐1‐based lentivirus was mixed with HL‐60 cells containing vector with δLf cDNA (HL‐60 δLf cells) . Successfully transformed cells (HL‐60 δLf/δANXA3 cells) were sorted using a BD FACSAriaII Cell Sorter.…”

Section: Methodsmentioning

confidence: 99%

“…RP S19 has a C5aR‐binding moiety (L 131 DR) similar to C5a and a specific C‐terminus sequence (I 134 AGQVAAANKKH). IAGQVAAANKKH interacts with the neutrophil‐specific transcription factor delta lactoferrin (δLF) and induces the expression of regulator of G protein signaling 3 (RGS3) . The GTPase activity of RGS3 inhibits G protein‐dependent downstream signaling .…”

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confidence: 99%

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Participation of delta annexin A3 in the ribosomal protein S19 C‐terminus‐dependent inhibitory mechanism of the neutrophil C5a receptor through delta lactoferrin

Yamanegi

Yamada²,

Nakasho³

et al. 2017

Pathology International

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Although C5a receptor (C5aR) interacting with its agonist C5a promotes acute inflammation during the initiation phase, the roles of the recycling C5aR during the resolution phase are still unclear. We found that C5aR interacted with its antagonist/agonist ribosomal protein S19 (RP S19) polymer or a RP S19 polymer functional analogue S-tagged C5a/RP S19, which connects an RP S19 C-terminus (IAGQVAAANKKH) to the S-tagged C5a C-terminus, promoted acute inflammation at the resolution phase via an activation of the apoptosis-inducing transcription factor delta lactoferrin (δLf) in neutrophils and the membrane mobilizing factor full-length annexin A3 (ANXA3) in macrophages. To confirm the antagonistic system of the recycling C5aR, S-tagged δLf-coupled BrCN-activated Sepharose 4B beads were incubated with cytoplasmic proteins and identified a neutrophil-specific δANXA3 via pull-down experiments. The S-tagged C5a/RP S19-induced agonistic functions in macrophage-like cells that were differentiated from human promyelocytic leukemia HL-60 cells by phorbol-12-myristate-13-acetate were suppressed by δLf and δANXA3 co-overexpression. δANXA3 seems to participate in the antagonistic system of the neutrophil C5aR involving IAGQVAAANKKH and δLf. Most likely, δANXA3 works as antagonist for the recycling C5aR on neutrophils during the resolution phase of acute inflammation.

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Section: Resultsmentioning

confidence: 75%

Section: Resultsmentioning

confidence: 75%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Participation of delta annexin A3 in the ribosomal protein S19 C‐terminus‐dependent inhibitory mechanism of the neutrophil C5a receptor through delta lactoferrin

Yamanegi

Yamada²,

Nakasho³

et al. 2017

Pathology International

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Biological Consequences of Protein Moonlighting

2016

Protein Moonlighting in Biology and Medicine

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RP S19 C-terminal peptide trimer acts as a C5a receptor antagonist

Nishiura

Kawakami

Kawabe

et al. 2016

Biochemistry and Biophysics Reports

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We have demonstrated that ribosomal protein S19 (RP S19) polymer, when crosslinked between Lys122 and Gln137 by activated coagulation factor XIII, acts as a C5a receptor (C5aR) antagonist/agonist. Based on experimental data obtained using RP S19 analog peptide and recombinant protein monomer, we suggested that L131DR, I134AGQVAAAN and K143KH moieties in the RP S19 C‐terminus act in, respectively, C5aR binding, penetration of the plasma membrane, and interaction with either an apoptosis-inducing molecule in neutrophils (delta lactoferrin) or a calcium channel-activating molecule (annexin A3) to induce the p38 MAPK pathway in macrophages. Recently, we observed RP S19 trimer in serum. To study the effects of this RP S19 trimer on C5aR, we prepared mutant RP S19 C‐terminal peptide (RP S19122-145) dimer and trimer, and examined their chemotactic activities and signal transduction pathways in human C5aR-overexpressing squamous cell carcinoma HSC-1 (HSC-1C5aR) cells using 24 trans-well chamber and western blotting assays, respectively. HSC-1C5aR cells were attracted by RP S19122-145 dimer and vice versa by RP S19122-145 trimer. The RP S19122-145 dimer-induced attraction was competitively blocked by pre-treatment with RP S19122-145 trimer. Moreover, RP S19122-145 trimer-induced p38 MAPK phosphorylation was stronger than RP S19122-145 dimer-induced p38 MAPK phosphorylation. RP S19122-145 trimer appeared to act as a C5aR antagonist. The agonistic and antagonistic effects of RP S19122-145 dimers and trimers were reflected by monocytic, THP-1-derived macrophage-like cells. Unlike the C5aR agonist C5a, which acts at the inflammation phase of acute inflammation, RP S19 trimer might act as a C5aR antagonist at the resolution phase.

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The roles of ribosomal protein S19 C-terminus in a shortened neutrophil lifespan through delta lactoferrin

Cited by 7 publications

References 42 publications

Participation of delta annexin A3 in the ribosomal protein S19 C‐terminus‐dependent inhibitory mechanism of the neutrophil C5a receptor through delta lactoferrin

Participation of delta annexin A3 in the ribosomal protein S19 C‐terminus‐dependent inhibitory mechanism of the neutrophil C5a receptor through delta lactoferrin

Biological Consequences of Protein Moonlighting

RP S19 C-terminal peptide trimer acts as a C5a receptor antagonist

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