The roles of plant dsRNA‐binding proteins in RNAi‐like pathways

Curtin, Shaun J.; Watson, John M.; Smith, Nerida Ann; Eamens, Andrew L.; Blanchard, Christopher L.; Waterhouse, Peter M.

doi:10.1016/j.febslet.2008.07.004

Cited by 96 publications

(162 citation statements)

References 35 publications

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“…The biased accumulation of miRNA duplex strands was also assessed in our dsRBP combinational insertion mutant knockout line, drb2345 (Curtin et al 2008). In this line, only one, DRB1, of the five known Arabidopsis dsRBPs is functional.…”

Section: Strand Choice From Amirna Duplexesmentioning

confidence: 99%

“…Furthermore, DCL4 exclusively interacts with DRB4, to efficiently process both viral and trans-acting siRNAs from their dsRNA substrates (Hiraguri et al 2005;Adenot et al 2006;Nakazawa et al 2007;Curtin et al 2008). Therefore, we studied the accumulation of these two miRNAs and their respective miRNA* sequences in drb1 and drb4 mutant plant lines (Fig.…”

Section: Strand Selection Of Srnas Produced By Dcl4/drb4mentioning

confidence: 99%

“…The dsRNA molecule is then processed in a phased manner, initiating at the miRNA cleavage site by DCL4/DRB4 to produce sequential 21-nt tasiRNAs that are loaded into AGO1 to direct cleavage of cognate mRNAs in trans (Gasciolli et al 2005;Xie et al 2005). In addition to tasiRNA biogenesis, the concerted action of DCL4/DRB4 is also required for the efficient production of the 21-nt size class of exogenous siRNAs derived from either replicating viruses or from an introduced hairpin RNA (hpRNA)-encoding transgene Curtin et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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The Arabidopsis thaliana double-stranded RNA binding protein DRB1 directs guide strand selection from microRNA duplexes

Eamens¹,

Smith²,

Curtin³

et al. 2009

RNA

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200

192

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In Arabidopsis thaliana (Arabidopsis), DICER-LIKE1 (DCL1) functions together with the double-stranded RNA binding protein (dsRBP), DRB1, to process microRNAs (miRNAs) from their precursor transcripts prior to their transfer to the RNA-induced silencing complex (RISC). miRNA-loaded RISC directs RNA silencing of cognate mRNAs via ARGONAUTE1 (AGO1)-catalyzed cleavage. Short interefering RNAs (siRNAs) are processed from viral-derived or transgene-encoded molecules of doublestranded RNA (dsRNA) by the DCL/dsRBP partnership, DCL4/DRB4, and are also loaded to AGO1-catalyzed RISC for cleavage of complementary mRNAs. Here, we use an artificial miRNA (amiRNA) technology, transiently expressed in Nicotiana benthamiana, to produce a series of amiRNA duplexes with differing intermolecular thermostabilities at the 59 end of duplex strands. Analyses of amiRNA duplex strand accumulation and target transcript expression revealed that strand selection (amiRNA and amiRNA*) is directed by asymmetric thermostability of the duplex termini. The duplex strand possessing a lower 59 thermostability was preferentially retained by RISC to guide mRNA cleavage of the corresponding target transgene. In addition, analysis of endogenous miRNA duplex strand accumulation in Arabidopsis drb1 and drb2345 mutant plants revealed that DRB1 dictates strand selection, presumably by directional loading of the miRNA duplex onto RISC for passenger strand degradation. Bioinformatic and Northern blot analyses of DCL4/DRB4-dependent small RNAs (miRNAs and siRNAs) revealed that small RNAs produced by this DCL/dsRBP combination do not conform to the same terminal thermostability rules as those governing DCL1/DRB1-processed miRNAs. This suggests that small RNA processing in the DCL1/DRB1-directed miRNA and DCL4/DRB4-directed sRNA biogenesis pathways operates via different mechanisms.

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Section: Strand Choice From Amirna Duplexesmentioning

confidence: 99%

Section: Strand Selection Of Srnas Produced By Dcl4/drb4mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Arabidopsis thaliana double-stranded RNA binding protein DRB1 directs guide strand selection from microRNA duplexes

Eamens¹,

Smith²,

Curtin³

et al. 2009

RNA

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200

192

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“…DCLs associate with dsRNA-binding proteins (DRB) which assist in the production of siRNAs [73]. In Arabidopsis, DRB4 operates with DCL4 to produce siRNAs from viral RNA [30]. The interaction of CDL4 and DRB4 in viral siRNA production has been shown in experiments both in vitro and in vivo [3].…”

Section: Function In Plant Immune Systemsmentioning

confidence: 99%

Diversity, evolution, and therapeutic applications of small RNAs in prokaryotic and eukaryotic immune systems

Cooper

Overstreet

2014

Physics of Life Reviews

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Recent evidence supports that prokaryotes exhibit adaptive immunity in the form of CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) and Cas (CRISPR associated proteins). The CRISPR-Cas system confers resistance to exogenous genetic elements such as phages and plasmids by allowing for the recognition and silencing of these genetic elements. Moreover, CRISPR-Cas serves as a memory of past exposures. This suggests that the evolution of the immune system has counterparts among the prokaryotes, not exclusively among eukaryotes. Mathematical models have been proposed which simulate the evolutionary patterns of CRISPR, however large gaps in our understanding of CRISPR-Cas function and evolution still exist. The CRISPR-Cas system is analogous to small RNAs involved in resistance mechanisms throughout the tree of life, and a deeper understanding of the evolution of small RNA pathways is necessary before the relationship between these convergent systems is to be determined. Presented in this review are novel RNAi therapies based on CRISPR-Cas analogs and the potential for future therapies based on CRISPR-Cas system components.

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“…DCLs' cofactors, the DRB proteins are also required for vsiRNA biogenesis. DRB4, the cofactor of DCL4, takes part in antiviral defense against RNA viruses (Curtin et al 2008;Jakubiec et al 2012;Qu et al 2008 silencing suppressor of Cauliflower mosaic virus (CaMV) inhibits DRB4, strongly suggests that DRB4 is an antiviral factor against DNA viruses as well. DRB3, the cofactor of DCL3, contributes to antiviral defense through chromatin modification against DNA viruses (Raja et al 2014).…”

Section: Biogenesis Of Vsirnasmentioning

confidence: 99%

Antiviral Silencing and Suppression of Gene Silencing in Plants

Csorba

Burgyán

2016

Current Research Topics in Plant Virology

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RNA silencing is an evolutionary conserved sequence-specific gene inactivation mechanism that contributes to the control of development, maintains heterochromatin, acts in stress responses, DNA repair and defends against invading nucleic acids like transposons and viruses. In plants RNA silencing functions as one of the main immune systems. RNA silencing process involves the small RNAs and trans factor components like Dicers, Argonautes and RNA-dependent RNA polymerases. To deal with host antiviral silencing responses viruses evolved mechanisms to avoid or counteract this, most notably through expression of viral suppressors of RNA silencing. Due to the overlap between endogenous and antiviral silencing pathways while blocking antiviral pathways viruses also impact endogenous silencing processes. Here we provide an overview of antiviral silencing pathway, host factors implicated in it and the crosstalk between antiviral and endogenous branches of silencing. We summarize the current status of knowledge about the viral counter-defense strategies acting at various steps during virus infection in plants with the focus on representative, well studied silencing suppressor proteins. Finally we discuss future challenges of the antiviral silencing and counter-defense research field.

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The roles of plant dsRNA‐binding proteins in RNAi‐like pathways

Cited by 96 publications

References 35 publications

The Arabidopsis thaliana double-stranded RNA binding protein DRB1 directs guide strand selection from microRNA duplexes

The Arabidopsis thaliana double-stranded RNA binding protein DRB1 directs guide strand selection from microRNA duplexes

Diversity, evolution, and therapeutic applications of small RNAs in prokaryotic and eukaryotic immune systems

Antiviral Silencing and Suppression of Gene Silencing in Plants

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