The roles of miRNAs as potential biomarkers in lung diseases

Alipoor, Shamila D.; Adcock, Ian M.; Garssen, Johan; Mortaz, Esmaeil; Varahram, Mohammad; Mirsaeidi, Mehdi; Velayati, A A

doi:10.1016/j.ejphar.2016.09.015

Cited by 131 publications

(118 citation statements)

References 140 publications

(164 reference statements)

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“…New findings confirm the important role of specific miRNAs in biological processes such as proliferation, cell differentiation and evolution, modulation of immune responses in macrophages, and differentiation of heart and muscle cells . The role of miRNAs in the pathogenesis of various pulmonary diseases, including pulmonary fibrosis, obstructive pulmonary disease, lung cancer as well as asthma has been proven . Among the miRNAs involved in the progression of asthma, miRNA‐146a can be mentioned.…”

Section: Introductionmentioning

confidence: 77%

Effect of high fat diet on NF‐кB microRNA146a negative feedback loop in ovalbumin‐sensitized rats

et al. 2018

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The present study aimed to investigate the role of microRNA‐146a and its adapter proteins [interleukin‐1 receptor‐associated kinase 1 (IRAK1) and tumor necrosis factor (TNF) receptor‐associated factor 6 (TRAF6)] in the pathogenesis of ovalbumin (OVA)‐sensitized rats in association with the diet‐induced obesity condition. Twenty male Wistar rats were divided into four groups: control with normal diet (ND), OVA‐sensitized with normal diet (S + ND), high‐fat diet (HFD), and OVA‐sensitized with high‐fat diet (S + HFD). All the animals were fed for 8 weeks with standard pelts or high‐fat diet, and were then sensitized and challenged with OVA or saline for another 4 weeks. The tracheal responsiveness to methacholine, serum protein levels, and lipid profile levels was measured by the ELISA method. Moreover, the gene expression level of microRNA‐146a (miR‐146a) was measured in the lung tissue of the rats using the real‐time PCR method. Maximum response to methacholin increased in the S + HFD group in compared with ND, S + ND, and HFD groups (P < 0.05 to P < 0.001). Moreover, in the S + HFD group the mRNA expression levels of miRNA‐146a increased in the lung tissue (P < 0.001). In addition, the protein analysis results showed that IRAK1, TRAF6, NF‐kB, and IL‐1β protein levels were high in the S + HFD group compared to the ND and HFD groups; however, in compared with the S + ND group, only the IL‐1β protein level was higher in the S + HFD group (P < 0.05). These results suggest that a defect in the NF‐kB–miR‐146a negative feedback loop may be involved in the pathogenesis of obesity associated with OVA‐sensitized condition. © 2018 BioFactors, 45(1):75–84, 2019

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Section: Introductionmentioning

confidence: 77%

Effect of high fat diet on NF‐кB microRNA146a negative feedback loop in ovalbumin‐sensitized rats

et al. 2018

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“…In addition, studies showed that also other population characteristics such as age, weight, smoking status, and ethnicity can affect the identification of miRNA markers . Even other benign diseases such as chronic obstructive pulmonary disease (COPD), asthma, and tuberculosis can alter blood miRNA profiles and make some study participants unsuitable controls . Therefore, in this systematic review, we selected Western populations and excluded studies with disease controls to reduce the heterogeneity of included miRNAs caused by above‐mentioned factors.…”

Section: Discussionmentioning

confidence: 99%

Circulating microRNA biomarkers for lung cancer detection in Western populations

Guan

Ćuk

et al. 2018

Cancer Medicine

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Lung cancer (LC) is a leading cause of cancer‐related death in the Western world. Patients with LC usually have poor prognosis due to the difficulties in detecting tumors at early stages. Multiple studies have shown that circulating miRNAs might be promising biomarkers for early detection of LC. We aimed to provide an overview of published studies on circulating miRNA markers for early detection of LC and to summarize their diagnostic performance in Western populations. A systematic literature search was performed in PubMed and ISI Web of Knowledge to find relevant studies published up to 11 August 2017. Information on study design, population characteristics, miRNA markers, and diagnostic accuracy (including sensitivity, specificity, and AUC) were independently extracted by two reviewers. Overall, 17 studies evaluating 35 circulating miRNA markers and 19 miRNA panels in serum or plasma were included. The median sensitivity (range) and specificity (range) were, respectively, 78.4% (51.7%‐100%) and 78.7% (42.9%‐93.5%) for individual miRNAs, and 83.0% (64.0%‐100%) and 84.9% (71.0%‐100%) for miRNA panels. Most studies incorporated individual miRNA markers as panels (with 2‐34 markers), with multiple miRNA‐based panels generally outperforming individual markers. Two promising miRNA panels were discovered and verified in prospective cohorts. Of note, both studies exclusively applied miRNA ratios when building up panels. In conclusion, circulating miRNAs may bear potential for noninvasive LC screening, but large studies conducted in screening or longitudinal settings are needed to validate the promising results and optimize the marker panels.

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“…Numerous studies have shown that miRNAs are involved in a wide variety of biological processes, including cell proliferation, differentiation, metastasis, apoptosis and immune responses [16][17][18][19]; moreover, they also function as prognostic markers in the development and progression of ischemic renal diseases by targeting pertinent genes [20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

MiR-155 is Involved in Renal Ischemia-Reperfusion Injury via Direct Targeting of FoxO3a and Regulating Renal Tubular Cell Pyroptosis

Huang²,

梁英³

et al. 2016

Cell Physiol Biochem

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Background/Aims: Ischemia/reperfusion injury (IRI) plays a crucial role in renal transplantation and can cause renal failure associated with pyroptosis, a pro-inflammatory-induced programmed cell death. Small endogenous non-coding RNAs have been shown to be involved in renal ischemia/reperfusion injury. This study was performed to investigate which miRNAs regulate pyroptosis in response to renal ischemia/reperfusion injury and determine the mechanism underlying this regulation. Methods: An in vivo rat model of renal IRI was established, and the serum and kidneys were harvested 24 h after reperfusion to assess renal function and histological changes. For the in vitro study, the cultured human renal proximal tubular cell line HK-2 was subjected to 24 h of hypoxia (5% CO₂, 1% O₂, and 94% N2) followed by 12 h of reoxygenation (5% CO₂, 21% O₂, and 74% N₂). The mRNA expression levels were analyzed by real-time PCR, and the protein expression levels were analyzed using Western blot, immunofluorescence staining and enzyme-linked immunosorbent assay (ELISA). Bioinformatics analyses were applied to predict miR-155 targets, which were then confirmed by a luciferase reporter assay. Results: We found that the levels of pyroptosis-related proteins, including caspase-1, caspase-11, IL-1β and IL-18, were significantly increased after renal ischemia/reperfusion injury. Similarly, hypoxia-reoxygenation injury (HRI) also induced pyroptosis in HK2 cells. Furthermore, our study revealed that miR-155 expression was substantially increased in the renal tissues of IRI rats and in HRI HK2 cells. Up-regulation of miR-155 promoted HK2 cell pyroptosis in HRI; conversely, knockdown of miR-155 attenuated this process. To understand the signaling mechanisms underlying the pro-pyroptotic activity of miR-155, we found that exogenous expression of miR-155 up-regulated the expression of caspase-1 as well as the pro-inflammatory cytokines IL-1β and IL-18. Moreover, miR-155 directly repressed FoxO3a expression and its downstream protein apoptosis repressor with caspase recruitment domain (ARC). Conclusions: Our study proposes a new signaling pathway of miR-155/FoxO3a/ARC leading to renal pyroptosis under ischemia/reperfusion injury conditions.

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The roles of miRNAs as potential biomarkers in lung diseases

Cited by 131 publications

References 140 publications

Effect of high fat diet on NF‐кB microRNA146a negative feedback loop in ovalbumin‐sensitized rats

Effect of high fat diet on NF‐кB microRNA146a negative feedback loop in ovalbumin‐sensitized rats

Circulating microRNA biomarkers for lung cancer detection in Western populations

MiR-155 is Involved in Renal Ischemia-Reperfusion Injury via Direct Targeting of FoxO3a and Regulating Renal Tubular Cell Pyroptosis

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