The roles of intracellular glutathione in antineoplastic chemotherapy

Differential effects of depleting agents on cytoplasmic and nuclear non-protein sulphydryls: a fluorescence image cytometry study

“…The level of cellular GSH, either constitutive or following biochemical manipulation, has been correlated with the relative susceptibility and resistance of tumour cells to ionising radiation and a variety of anti-tumour agents (Arrich and Nathan, 1984;Hamilton et al. 1986;Russo et al, 1986;Mistry and Harrap, 1991). In tissue culture GSH is usually considered to be the dominant non-protein sulphydryl (NPSH) involved in drug and radiation resistance.…”

Effect of glutathione depletion on the cytotoxicity of antitumor drugs to cultured human tumor cell lines.

“…In contrast, Russo et al 24 have reported that BLM cytotoxicity can be increased by GSH depletion by using a higher dose of BSO (10 mM, 4 h). Moreover, Andrews et al 17 have suggested that there are 2 types of ovarian carcinoma cells, one is more sensitive to CD-DP by GSH depletion than the control and the other one is not.…”

Enhanced cytostatic activity of the sesquiterpene lactone eupatoriopicrin by glutathione depletion

“…Bone marrow is an example of normal tissue that has been found to be depleted by BSO to only moderate values (Russo et al, 1986;Soble & Dorr, 1987). With the dosage regimen used in our experiments, GSH levels in the tumours were severely reduced by BSO at the moment of supplying EUP, while the GSH status of the liver was already recovering.…”

“…Reviews on this topic have recently been published (Meister & Anderson, 1983;Arrick & Nathan, 1984). However, cytostatics acting independently from GSH (5-fluorouracil, vincristine), are not potentiated by GSH depletion (Ozols & Cowan, 1986) and the effect of antineoplastic agents that require intracellular activation by sulphydryl groups (neocarzinostatin) is absent after GSH depletion (Russo et al, 1986).…”