The roles of GSTs in fipronil resistance in Nilaparvata lugens: Over-expression and expression induction

Gao, Haoli; Lin, Xiangze; Yang, Bob; Liu, Zewen

doi:10.1016/j.pestbp.2021.104880

Cited by 26 publications

(10 citation statements)

References 24 publications

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“…Synergism bioassay indicated the contribution of GSTs in the susceptibility to these three insecticides in G. molesta larvae (Figure 1b). In Nilaparvata lugens and Aphis craccivora , GSTs were also demonstrated to involve in the insecticide resistance of fipronil and imidacloprid by using synergism bioassay of GSTs inhibitor DEM (Gao et al, 2021; Yang, Lin, Li, et al, 2020; Yang, Lin, Yu, et al, 2020). To identify which G. molesta GST genes contribute to the insecticide susceptibility, we used qRT‐PCR and identified six GmGST genes were upregulated after exposure to EB, CT and LC from 21 GmGST genes.…”

Section: Discussionmentioning

confidence: 99%

Molecular identification of glutathione S‐transferase genes and their potential roles in insecticides susceptibility of Grapholita molesta

Zhang

Jia

et al. 2023

J Applied Entomology

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Grapholita molesta is one kind of serious fruit‐boring pests in the world, which caused huge economic losses to the fruit industry including pear and apple. The heavy usage of synthetic insecticides resulted in different degrees of resistance of G. molesta to some commonly used insecticides. However, the tolerance mechanisms of G. molesta to insecticides are still unclear. Glutathione S‐transferases (GSTs) belong to the superfamily of detoxifying enzymes and mainly play important roles in detoxification metabolism and insecticide susceptibility of insects. In this study, we first evaluated the toxicity of three insecticides including emamectin benzoate, chlorantraniliprole and lambda‐cyhalothrin on G. molesta at different developmental stages. Synergism study showed that the susceptibility of G. molesta larvae to these three insecticides obviously increased when the enzyme activity of GST was inhibited by diethyl maleate compared with the controls. Next, we screened out six GmGST genes which are upregulated after insecticides treatment from a total of 21 GmGST genes and analysed their sequence characteristics and expression profiles. RNAi and bioassay results further revealed that the mortality of 5th instar larvae significantly increased after exposure to LC30 of emamectin benzoate, chlorantraniliprole and lambda‐cyhalothrin when silencing of GmGSTo2, GmGSTs1 and GmGSTz1 compared with the controls. In conclusion, this study indicated that GmGSTo2, GmGSTs1 and GmGSTz1 play important roles in insecticides susceptibility of G. molesta to three commonly used insecticides, which is significance for its future integrated management.

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Section: Discussionmentioning

confidence: 99%

Molecular identification of glutathione S‐transferase genes and their potential roles in insecticides susceptibility of Grapholita molesta

Zhang

Jia

et al. 2023

J Applied Entomology

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“…Detoxification or sequestration of plant allelochemicals and insecticides is considered one of the adaptive mechanisms by which insects develop resistance, and cytochrome P450s (P450s), glutathione S ‐transferases (GSTs) and esterases (ESTs) play a primary role in this process 3,5,6 . Overexpression of P450s, GSTs and ESTs genes can enhance the metabolism of insecticides such as pyrethroids and neonicotinoids in many common agricultural pests, such as Plutella xylostella , Helicoverpa armigera , Cydia pomonella and Nilaparvata lugens 7–10 . In particular, the up‐regulation of three GST Delta family genes ( BdGSTd1 , BdGSTd9 , BdGSTd10 ) or the presence of a single nucleotide polymorphism in BdGSTe8 enhanced the detoxification of malathion in B. dorsalis 11–13 .…”

Section: Introductionmentioning

confidence: 99%

“…3,5,6 Overexpression of P450s, GSTs and ESTs genes can enhance the metabolism of insecticides such as pyrethroids and neonicotinoids in many common agricultural pests, such as Plutella xylostella, Helicoverpa armigera, Cydia pomonella and Nilaparvata lugens. [7][8][9][10] In particular, the upregulation of three GST Delta family genes (BdGSTd1, BdGSTd9, BdGSTd10) or the presence of a single nucleotide polymorphism in BdGSTe8 enhanced the detoxification of malathion in B. dorsalis. [11][12][13] UDP-glycosyltransferases (UGTs) act together with GSTs as secondary detoxifiers of both endogenous and exogenous substances in plants, vertebrates and invertebrates.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of UDP‐glycosyltransferase genes and the potential role in response to insecticides exposure in Bactrocera dorsalis

Chen

Zhang

Guo

et al. 2022

Pest Management Science

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BACKGROUND The oriental fruit fly, Bactrocera dorsalis (Hendel) is a worldwide pest damaging a wide range of hosts. Due to the long‐term indiscriminate use of insecticides, B. dorsalis has developed serious resistance to several insecticides. UDP‐glycosyltransferases (UGTs) are secondary metabolic enzymes involved in biotransformation and play an important role in the metabolism of plant secondary metabolites and synthetic insecticides in insects. Thus, we suspect that UGTs in B. dorsalis play an important role in insecticide tolerance. RESULTS In this study, 31 UGT genes were identified in the genome of B. dorsalis, belonging to 13 subfamilies. Real‐time quantitative polymerase chain reaction (RT‐qPCR) results revealed that 12 UGT genes were highly expressed in the antennae, midgut, Malpighian tubule and fat body. The mRNA expressions of 17 UGT genes were up‐regulated upon exposure to λ‐cyhalothrin, imidacloprid, abamectin and chlorpyrifos. Knockdown of the selected five UGT genes (BdUGT301D2, BdUGT35F2, BdUGT36K2, BdUGT49D2, BdUGT50B5) by RNA interference increased the mortality of B. dorsalis from 9.29% to 27.22% upon exposure to four insecticides. CONCLUSION The abundance of UGTs in B. dorsalis is similar to other insect species, and 12 out of 31 UGTs were specifically expressed in metabolic tissues, suggesting a key role in detoxification. Down‐regulation of five selected UGT genes increased the susceptibility of B. dorsalis to various insecticides, indicating that UGTs may play an important role in tolerance of B. dorsalis to multiple insecticides. © 2022 Society of Chemical Industry.

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“…The long-term use of carbamates, organophosphates, pyrethroids, and neonicotinoids has led to widespread resistance in BPH. , Flonicamid, a novel selective pesticide to control BPH, can inhibit the feeding of piercing–sucking pests, causing starvation and even death. , Recently, a possible target Naam for flonicamid has been identified . However, the mechanism by which flonicamid interferes with salivary secretion in BPH is still unclear.…”

Section: Discussionmentioning

confidence: 99%

Reducing Expression of Salivary Protein Genes by Flonicamid Partially Contributed to Its Feeding Inhibition of the Brown Planthopper on Rice

Gao

Yuan

Lin

et al. 2023

J. Agric. Food Chem.

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Flonicamid inhibits the feeding of piercing−sucking pests as a selective systemic insecticide. The brown planthopper (BPH), Nilaparvata lugens (Stål), is one of the most serious pests on rice. During feeding, it uses its stylet to collect sap by penetrating the phloem, and at the same time, it delivers saliva into the rice plant. Insect salivary proteins play important roles in feeding and interacting with plants. Whether flonicamid affects the expression of salivary protein genes and then inhibits the feeding of BPH is not clear. Here, from 20 functionally characterized salivary proteins, we screened five salivary proteins (NlShp, NlAnnix5, Nl16, Nl32, and NlSP7) whose gene expressions were significantly inhibited by flonicamid. We performed experimental analysis on two of them (Nl16 and Nl32). RNA interference of Nl32 significantly reduced the survival rate of BPH. Electrical penetration graph (EPG) experiments showed that both flonicamid treatment and knockdown of Nl16 and Nl32 genes significantly reduced the feeding activity of N. lugens in the phloem and also reduced the honeydew excretion and fecundity. These results suggested that the inhibition of flonicamid on the feeding behavior in N. lugens might be partially attributed to its effect on the expression of salivary protein genes. This study provides a new insight into the mechanism of action of flonicamid on insect pests.

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The roles of GSTs in fipronil resistance in Nilaparvata lugens: Over-expression and expression induction

Cited by 26 publications

References 24 publications

Molecular identification of glutathione S‐transferase genes and their potential roles in insecticides susceptibility of Grapholita molesta

Molecular identification of glutathione S‐transferase genes and their potential roles in insecticides susceptibility of Grapholita molesta

Identification and characterization of UDP‐glycosyltransferase genes and the potential role in response to insecticides exposure in Bactrocera dorsalis

Reducing Expression of Salivary Protein Genes by Flonicamid Partially Contributed to Its Feeding Inhibition of the Brown Planthopper on Rice

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