The Roles of Cellular Nanomechanics in Cancer

Yallapu, Murali M.; Katti, Kalpana S.; Katti, Dinesh R.; Mishra, Sanjay R.; Khan, Sheema; Jaggi, Meena; Chauhan, Subhash C.

doi:10.1002/med.21329

Cited by 39 publications

(30 citation statements)

References 121 publications

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“…Recent experimental evidence [15][16][17][18] have revealed that tumor cells exhibit a broad distribution of various biomechanical properties. These include intra-tumor heterogeneity in cell stiffnesses [17,[19][20][21][22], stresses, and cell-cell interactions [21,23].…”

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confidence: 99%

Mechanical Heterogeneity in Tissues Promotes Rigidity and Controls Cellular Invasion

Das

2019

Phys. Rev. Lett.

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We study the influence of cell-level mechanical heterogeneity in epithelial tissues using a vertex-based model. Heterogeneity in single cell stiffness is introduced as a quenched random variable in the preferred shape index(p 0 ) for each cell. We uncovered a crossover scaling for the tissue shear modulus, suggesting that tissue collective rigidity is controlled by a single parameter f r , which accounts for the fraction of rigid cells. Interestingly, the rigidity onset occurs at f r = 0.21, far below the contact percolation threshold of rigid cells. Due to the separation of rigidity and contact percolations, heterogeneity can enhance tissue rigidity and gives rise to an intermediate solid state. The influence of heterogeneity on tumor invasion dynamics is also investigated. There is an overall impedance of invasion as the tissue becomes more rigid. Invasion can also occur in the intermediate heterogeneous solid state and is characterized by significant spatial-temporal intermittency.

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confidence: 99%

Mechanical Heterogeneity in Tissues Promotes Rigidity and Controls Cellular Invasion

Das

2019

Phys. Rev. Lett.

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“…In particular, elastic modulus of tumors is an order of magnitude higher than elastic modulus of healthy tissues 23,24. Thus, tactile examination is a natural and efficient method for identification and localization of pathological changes.…”

Section: Discussionmentioning

confidence: 99%

Instrumental tactile diagnostics in robot-assisted surgery

Solodova¹,

Galatenko²,

Накашидзе³

et al. 2016

MDER

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BackgroundRobotic surgery has gained wide acceptance due to minimizing trauma in patients. However, the lack of tactile feedback is an essential limiting factor for the further expansion. In robotic surgery, feedback related to touch is currently kinesthetic, and it is mainly aimed at the minimization of force applied to tissues and organs. Design and implementation of diagnostic tactile feedback is still an open problem. We hypothesized that a sufficient tactile feedback in robot-assisted surgery can be provided by utilization of Medical Tactile Endosurgical Complex (MTEC), which is a novel specialized tool that is already commercially available in the Russian Federation. MTEC allows registration of tactile images by a mechanoreceptor, real-time visualization of these images, and reproduction of images via a tactile display.Materials and methodsNine elective surgeries were performed with da Vinci™ robotic system. An assistant performed tactile examination through an additional port under the guidance of a surgeon during revision of tissues. The operating surgeon sensed registered tactile data using a tactile display, and the assistant inspected the visualization of tactile data. First, surgeries where lesion boundaries were visually detectable were performed. The goal was to promote cooperation between the surgeon and the assistant and to train them in perception of the tactile feedback. Then, instrumental tactile diagnostics was utilized in case of visually undetectable boundaries.ResultsIn robot-assisted surgeries where lesion boundaries were not visually detectable, instrumental tactile diagnostics performed using MTEC provided valid identification and localization of lesions. The results of instrumental tactile diagnostics were concordant with the results of intraoperative ultrasound examination. However, in certain cases, for example, thoracoscopy, ultrasound examination is inapplicable, while MTEC-based tactile diagnostics can be efficiently utilized.ConclusionThe study proved that MTEC can be efficiently used in robot-assisted surgery allowing correct localization of visually undetectable lesions and visually undetectable boundaries of pathological changes of tissues.

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“…Atomic force microscopy (AFM) based single-cell force spectroscopy is an emerging method to directly investigate cellular mechanics and the dynamics of intercellular interactions (8,9). Excluding the immobilization of the melanoma cell, during these measurements there is no need for any staining or other cell-life impairing preparation.…”

Section: Introductionmentioning

confidence: 99%

De-adhesion dynamics of melanoma cells from brain endothelial layer

Varga

Domokos

Fazakas

et al. 2018

Biochimica et Biophysica Acta (BBA) - General Subjects

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Metastasis formation is a complex and not entirely understood process. The poorest prognosis and the most feared complications are associated to brain metastases. Melanoma derived brain metastases show the highest prevalence. Due to the lack of classical lymphatic drainage, in the process of brain metastases formation the haematogenous route is of primordial importance. The first and crucial step in this multistep process is the establishment of firm adhesion between the blood travelling melanoma cells and the tightly connected layer of the endothelium, which is the fundamental structure of the blood-brain barrier. This study compares the de-adhesion properties and dynamics of three melanoma cells types (WM35, A2058 and A375) to a confluent layer of brain micro-capillary endothelial cells. Cell type dependent adhesion characteristics are presented, pointing towards the existence of metastatic potential related nanomechanical aspects. Apparent mechanical properties such as elasticity, maximal adhesion force, number, size and distance of individual rupture events showed altered values pointing towards cell type dependent aspects. Our results underline the importance of mechanical details in case of intercellular interactions. Nevertheless, it suggests that in adequate circumstances elastic and adhesive characterizations might be used as biomarkers.

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The Roles of Cellular Nanomechanics in Cancer

Cited by 39 publications

References 121 publications

Mechanical Heterogeneity in Tissues Promotes Rigidity and Controls Cellular Invasion

Mechanical Heterogeneity in Tissues Promotes Rigidity and Controls Cellular Invasion

Instrumental tactile diagnostics in robot-assisted surgery

De-adhesion dynamics of melanoma cells from brain endothelial layer

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