The roles of BUB1/BUB1B/BUB3 in human breast cancer

Abstract

Breast cancer (BC), the most commonly diagnosed invasive cancer in females globally, has upset human being for a long time. We used GEPIA2, bc-GenExMiner v4.8, cBioPortal database, Kaplan-Meier Plotter and TIMER2.0 to explore expressions, clinical features, mutations, prognosis and immune infiltrations of benzimidazole 1 (BUB1)/ benzimidazole 1 mitotic checkpoint serine or threonine kinase B (BUB1B)/ benzimidazole 3 (BUB3) of BC. Nomogram also used to give a more precise prognostic value of breast invasive carcinoma (BRCA). BUB1/BUB1B/BUB3 all demonstrate significant predictive values in the prognostic of OS in BRCA, especially that of BUB1B. Moreover, the predictive value of BUB1 is just the opposite to that of BUB1B and BUB3. GO/KEGG analyses were conducted to analyse the mechanisms in biology. STRING and GeneMANIA were used to depicted co-expression genes with BUB1/BUB1B/BUB3 and 5 Hub genes were found upregulated in BC for further analyses. Our results show that in BRCA the BUB1/BUB1B/BUB3 expressions are upregulated and the expressions of BUB1/BUB1B correlate with the stages. Some clinicopathological features of the expressions of BUB3 are just the opposite of that in BUB1 and BUB1B. cBioPortal analyses show that only the minority of samples in BRCA go through mutations. Survival analyses reveal that BUB1 and BUB1B overexpression are associated with poor prognosis in BC, while BUB3 seems to imply longer RFS in triple negative breast cancer (TNBC). Moreover, BUB1/BUB1B/BUB3 expressions negatively correlate with different types of immune cells, and positive relations can be observed just in BUB3. GO and KEGG analyses illustrate that BUB1/BUB1B/BUB3 participate in the processes of organelle fission, chromosomal region, ATPase activity, cell cycle and so forth. The 5 Hub genes imply poor prognosis. BUB1/BUB1B/BUB3 are potential new biomarkers for BC patients.