The roles of Arabidopsis proteins of Lhcb4, Lhcb5 and Lhcb6 in oxidative stress under natural light conditions

Chen, Yang-Er; Ma, Jie; Wu, Nan; Su, Yan-Qiu; Zhang, Zhongwei; Yuan, Ming; Zhang, Huaiyu; Zeng, Xianyin; Yuan, Shu

doi:10.1016/j.plaphy.2018.07.014

Cited by 44 publications

(25 citation statements)

References 53 publications

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“…Higher plants capture the energy of light through a photosynthetic antenna system composed of light-harvesting complexes (LHC), and LHC proteins absorb light energy, transfers the excitation energy to the reaction center for a photochemical reaction, and store it in a chemical form for use by plants [70,71]. Six genes encoding LHC protein complexes, including LHCA1, LHCB1.4, LHCB3, LHCB4.2, and LHCB6, play important positive regulatory roles in light capture [72][73][74]. LHCA1, LHCA2, and LHCA5 can all transfer energy to the photosystem I complex [75].…”

Section: Differential Expression Of Genes Related To Photosynthesis and Carbon Fixation In Tetraploid Poplar Leavesmentioning

confidence: 99%

Study on Gene Differential Expression in Tetraploid Populus Leaves

Zhang

Ren

Kang

2020

Forests

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Polyploids exhibit different phenotypes compared to those of diploids in plants, and the important role of polyploids in tree breeding has been widely recognized. The transcriptomes detected by RNA-seq in the Populus triploid by doubling the chromosomes of the female gamete, in the triploid by doubling the chromosomes of somatic cells and the diploid with the parent were compared to reveal the patterns of gene expression of tetraploid leaves and their influence on growth. The results showed that the high expression of GATA and PORA in tetraploid leaves was the reason for the higher chlorophyll content in the leaves than in diploid and triploid leaves. The 11-day-old tetraploid leaves began to enter the aging stage. Compared with that in the diploid, GRF was significantly upregulated, while the amylase genes were downregulated. Compared with those in the triploid, 3 STN7 genes that regulate photosynthetic genes and PGSIP genes which are related to starch synthesis, were significantly downregulated in the tetraploid, and the auxin receptor protein TIR1 was also significantly downregulated. In the tetraploid, auxin-regulating genes such as GH3 and AUX/IAA as well as genes involved in the regulation of leaf senescence, SAG genes and SRG genes were significantly up-regulated, resulting in a decrease in the auxin content. In senescent leaves, CHLD, CHLI1, and CHLM in the early stage of chlorophyll synthesis all began to downregulate their expressions, leading to the downregulation of LHC genes and a decrease in their photosynthetic efficiency, which led to the downregulation of carbon fixation-related genes such as SS genes, thus affecting carbon synthesis and fixation. This finally led to the slow growth of tetraploid plants. These data represent the transcriptome characteristics of tetraploid, and they can be used as a resource for further research on polyploids and provide a reference for further understanding of the function of polyploid vegetative growth-related genes.

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Section: Differential Expression Of Genes Related To Photosynthesis and Carbon Fixation In Tetraploid Poplar Leavesmentioning

confidence: 99%

Study on Gene Differential Expression in Tetraploid Populus Leaves

Zhang

Ren

Kang

2020

Forests

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“…In lhcb6 mutant plants, we have found again a slight increase in the amount of LHCB1 and LHCB2 ( Figure 2, A ), which is in agreement with previously observed trends ( Kovács et al, 2006 ; de Bianchi et al, 2008 ). The level of LHCB3 did not change ( Figure 2, A ), although literature suggests a decrease to 70% ( Kovács et al, 2006 ; Chen et al, 2018 ) or even 25% ( de Bianchi et al, 2008 ) of the WT level. LHCB4 and LHCB5 did not show any distinct change in abundance ( Figure 2, A ).…”

Section: Resultsmentioning

confidence: 80%

“…Closer examination of the T-DNA insertion site in the SALK_077953 line reveals that the T-DNA insertion is localized in the 5’-UTR region of the lhcb6 gene (AT1G15820), which frequently leads to knockdowns rather than knockouts ( Wang, 2008 ). Other authors who have previously used this insertion line and performed Western blots either failed to detect this residual amount of LHCB6 ( de Bianchi et al, 2008 ; Chen et al, 2018 ), or observed it but disregarded it as non-detectable (3% ± 1% of wild-type (WT) level, Kovács et al, 2006 ), and therefore the insertion line has been widely used as a knockout mutant for LHCB6. Nevertheless, we have confirmed the presence of the residual amount of LHCB6 in the presumed knockout line also by the mass spectrometry analysis, which revealed a low, but unequivocally detectable amount of LHCB6 in both lhcb6 and lhcb3 lhcb6 (4%–5% of WT level, Figure 2, A ).…”

Section: Resultsmentioning

confidence: 99%

Towards spruce-type photosystem II: consequences of the loss of light-harvesting proteins LHCB3 and LHCB6 in Arabidopsis

et al. 2021

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The largest stable photosystem II (PSII) supercomplex in land plants (C2S2M2) consists of a core complex dimer (C2), two strongly (S2) and two moderately (M2) bound light-harvesting protein (LHCB) trimers attached to C2 via monomeric antenna proteins LHCB4-6. Recently, we have shown that LHCB3 and LHCB6, presumably essential for land plants, are missing in Norway spruce (Picea abies), which results in a unique structure of its C2S2M2 supercomplex. Here, we performed structure-function characterization of PSII supercomplexes in Arabidopsis (Arabidopsis thaliana) mutants lhcb3, lhcb6 and lhcb3 lhcb6 to examine the possibility of the formation of the “spruce-type” PSII supercomplex in angiosperms. Unlike in spruce, in Arabidopsis both LHCB3 and LHCB6 are necessary for stable binding of the M trimer to PSII core. The “spruce-type” PSII supercomplex was observed with low abundance only in the lhcb3 plants and its formation did not require the presence of LHCB4.3, the only LHCB4-type protein in spruce. Electron microscopy analysis of grana membranes revealed that the majority of PSII in lhcb6 and namely in lhcb3 lhcb6 mutants were arranged into C2S2 semi-crystalline arrays, some of which appeared to structurally restrict plastoquinone diffusion. Mutants without LHCB6 were characterized by fast induction of non-photochemical quenching and, on the contrary to the previous lhcb6 study, by only transient slowdown of electron transport between photosystems II and I. We hypothesize that these functional changes, associated with the arrangement of PSII into C2S2 arrays in thylakoids, may be important for the photoprotection of both PSI and PSII upon abrupt high-light exposure.

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“…The gene TraesCS2A01G342600 is a homologue of gene Lhcb6, which encodes a protein that is part of the light harvesting complex. It captures photons and transfers energy to the reaction center of the chlorophyll molecule [54]. At the same time, it has a protective function against reactive oxygen species.…”

Section: Discussionmentioning

confidence: 99%

Regions of Chromosome 2A of Bread Wheat (Triticum aestivum L.) Associated with Variation in Physiological and Agronomical Traits under Contrasting Water Regimes

Пшеничникова

Osipova

Smirnova

et al. 2021

Plants

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Understanding the genetic architecture of drought tolerance is of great importance for overcoming the negative impact of drought on wheat yield. Earlier, we discovered the critical role of chromosome 2A for the drought-tolerant status of wheat spring cultivar Saratovskaya 29. A set of 92 single-chromosome recombinant double haploid (SCRDH) lines were obtained in the genetic background of Saratovskaya 29. The lines carry fragments of chromosome 2A from the drought-sensitive cultivar Yanetzkis Probat. The SCRDH lines were used to identify regions on chromosome 2A associated with the manifestation of physiological and agronomical traits under distinct water supply, and to identify candidate genes that may be associated with adaptive gene networks in wheat. Genotyping was done with Illumina Infinium 15k wheat array using 590 SNP markers with 146 markers being polymorphic. In four identified regions of chromosome 2A, 53 out of 58 QTLs associated with physiological and agronomic traits under contrasting water supply were mapped. Thirty-nine candidate genes were identified, of which 18 were transcription factors. The region 73.8–78.1 cM included the largest number of QTLs and candidate genes. The variation in SNPs associated with agronomical and physiological traits revealed among the SCRDH lines may provide useful information for drought related marker-assisted breeding.

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The roles of Arabidopsis proteins of Lhcb4, Lhcb5 and Lhcb6 in oxidative stress under natural light conditions

Cited by 44 publications

References 53 publications

Study on Gene Differential Expression in Tetraploid Populus Leaves

Study on Gene Differential Expression in Tetraploid Populus Leaves

Towards spruce-type photosystem II: consequences of the loss of light-harvesting proteins LHCB3 and LHCB6 in Arabidopsis

Regions of Chromosome 2A of Bread Wheat (Triticum aestivum L.) Associated with Variation in Physiological and Agronomical Traits under Contrasting Water Regimes

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