The role played by endogenous and exogenous electric fields in DNA signaling and repair

Arruda-Neto, João D T; Friedberg, Errol C.; Bittencourt-Oliveira, M. C.; Segreto, Helena Regina Cômodo; Moron, M.M.; Maria, Durvanei Augusto; Batista, Luís F.Z.; Schenberg, Ana Clara Guerrini

doi:10.1016/j.dnarep.2009.12.023

Cited by 13 publications

(7 citation statements)

References 3 publications

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“…A comparatively new branch of radiotherapy focusing on the effect of the cascade electrons on the tumor cells is known as Auger therapy [6]. Finally, the cascade decays of inner-shell vacancies in heavy atoms can be quite long, contain many non-radiative decay steps, and thus lead to the production of rather highly charged final ions; the electric field of such cascadeproduced ions can also contribute to the damage to tumor cells due to its effect on the DNA repair cycle [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Final ion yields upon the cascade decay of single K, L, M, and N vacancies in atomic silver

et al. 2023

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Final ion yields and mean final ion charges upon the cascade decay of single vacancies in the K, L, M, and N shells of atomic silver are calculated using the method of construction and analysis of the decay trees with accounting for the shake-offs caused by the change of atomic core potential due to cascade transitions. The shake-off effect on the final ion yields is found to be comparatively small, the largest increase of the mean final ion charge being 0.20e in the case of initial 2s vacancy. Total semi-relativistic Pauli–Fock energies of cascade-produced ionic configurations were used to calculate cascade transition energies, which made it possible to accurately account for the closing of some non-radiative transitions in multivacancy cascade configurations. For the transitions with overlapping multiplets of initial and final ionic configurations, only energetically allowed transitions between the multiplet components were counted when calculating mean transition energies and transitions branching ratios. Comparison of calculated charge spectra with those reported earlier by other authors demonstrated crucial importance of accurate calculation of transition energies and accounting for multiplet splitting in simulation of cascades in heavy atoms.

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Section: Introductionmentioning

confidence: 99%

Final ion yields upon the cascade decay of single K, L, M, and N vacancies in atomic silver

et al. 2023

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“…Previous experimental results from our research group have shown that organisms submitted to gamma radiation and immediately after exposed exposure to a SEF demonstrate the persistence of the γH2AX histone [29], thus revealing the ability of SEFs to inhibit repair, while since the persistence of γH2AX reflects the presence of unrepaired DNA damage [30][31][32]. As with ionizing radiation, cells treated with VP-16 also show a response in the MRN complex which works in the ATR pathway, in response to various DNA damaging agents [31,33], revealing a connection with the response factors of repair via ATM and ATR which, similarly to γH2AX, work within a few minutes after the application of VP-16 [33,34].…”

Section: Discussionmentioning

confidence: 77%

Static Electric Field Increases In Vitro Apoptosis of the Etoposide Chemotherapeutic Agent

Ra¹,

Faião-Flores²,

Df³

et al. 2019

Oncol Res Rev

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Tumor cells (murine melanoma) and human fibroblasts were simultaneously exposed to the chemotherapeutic agent etoposide (VP-16) and a static electric field (SEF) in a single or combinatory therapy. Single exposure to a SEF showed no significant differences in cell viability, whereas the combination with VP-16 induced apoptosis increases in both cell lines. Single VP-16 treatment induced apoptosis for up to 6 h, while the combination of both VP-16 and SEF extended apoptotic activity throughout for up to 24 h of exposure. The combined results of SEF with VP-16 induced a substantial increase of cell deaths, reducing VP-16 IC 50 from 13 µM to 5.1 µM in melanoma cells, and from 12.6 µM to 3.2 µM in the fibroblast cells. Detection of apoptosis via Anexina V/PI and caspase 3 showed a 100% marks increase. Taken all together, the present results strongly indicate that SEF dramatically increases VP-16 apoptosis, which makes this physical agent a strong chemotherapeutic sensitizer.

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“…A series of experiments was carried out at this Laboratory with prokaryotes and eukaryotes exposed to ionizing radiation and exogenous static electric fields. [22][23][24][25] All results show that when cells exposed to radiation are immediately submitted to static electric fields, cell death increases enormously compared to the effect of radiation alone. Since the sole application of an electric (E) field has no effect on control samples, it was thus demonstrated that the E-field acts on the DNA repair process.…”

Section: Navigation/recognitionmentioning

confidence: 99%

“…ATM in mammalian cells, MEC1 in S. cerevisiae and RAD3 in S. pombe, is induced by an electromagnetic (EM) transient emitted from the DNA damaged site. 23 While providing a conceptually correct cause-effect relationship for the primary DNA signaling process, this hypothesis points to the very likely possibility that EM fields initiate phosphorylation by interacting directly with moving electrons in proteins. This is precisely the analog of electromagnetic initiation of DNA transcription.…”

Section: Signaling/sensingmentioning

confidence: 99%

Sensing Of DNA Damage, Instantly Activation of Repairing Proteins and Radio Sensitizers-A Biophysical Model

Jdt¹

2015

MOJPB

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The physical nature of mechanisms by which cells sense radiation-induced DNAstrand breaks remains to be elucidated. The fast induction of, for example, ATM kinase activity immediately after exposure to ionizing radiation, suggests that it acts at an early stage of signal transduction. Existing data indicate that ATM activation is not dependent on direct binding to strand breaks. However, some fundamental questions are still not responded to, such as: how ATM directly senses structure disruption in "relaxed" chromatin, and which factors determine the impressive speed and extent of the ATM response. Here, a biophysical model for the signaling mechanism of both the instant activation of repair proteins, and the recognition of a few DNA breaks by these proteins within the entire genome is proposed. The model allows for the calculation of an electromagnetic transient, produced by an electron coherent hopping current generated by the DNA damage, which is able to induce long-range activation (phosphorylation) of repair proteins almost instantly. Existing experimental evidence verifying this approach is discussed. The possible role of this study in stimulating and orientating novel applications in medical physics, as e.g. radiotherapy, is, furthermore, addressed. Citation: Arruda-Neto JDT. Sensing of DNA damage, instantly activation of repairing proteins and radio sensitizers-a biophysical model. MOJ Proteomics Bioinform. 2015;2(5):165-169. Citation: Arruda-Neto JDT. Sensing of DNA damage, instantly activation of repairing proteins and radio sensitizers-a biophysical model. MOJ Proteomics Bioinform. 2015;2(5):165-169.

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The role played by endogenous and exogenous electric fields in DNA signaling and repair

Cited by 13 publications

References 3 publications

Final ion yields upon the cascade decay of single K, L, M, and N vacancies in atomic silver

Final ion yields upon the cascade decay of single K, L, M, and N vacancies in atomic silver

Static Electric Field Increases In Vitro Apoptosis of the Etoposide Chemotherapeutic Agent

Sensing Of DNA Damage, Instantly Activation of Repairing Proteins and Radio Sensitizers-A Biophysical Model

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