The role of yeastARO8, ARO9andARO10genes in the biosynthesis of 3-(methylthio)-1-propanol fromL-methionine during fermentation in synthetic grape medium

Deed, Rebecca C.; Hou, Ruoyu; Kinzurik, Matias I.; Gardner, Richard C.; Fedrizzi, Bruno

doi:10.1093/femsyr/foy109

Cited by 28 publications

(34 citation statements)

References 55 publications

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“…The OD 600 was then determined using a spectrophotometer. Glucose consumption was determined by the DNS method ( Deed et al, 2018 ). One milliliter of culture was centrifuged for 10 min at 12000 rpm at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Zygosaccharomyces rouxii, an Aromatic Yeast Isolated From Chili Sauce, Is Able to Biosynthesize 2-Phenylethanol via the Shikimate or Ehrlich Pathways

Dai

Song

et al. 2020

Front. Microbiol.

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We isolated an aromatic strain of yeast (M2013310) from chili sauce. Assembly, annotation, and phylogenetic analysis based on genome sequencing, identified M2013310 as an allodiploid yeast that was closely related to Zygosaccharomyces rouxii . During fermentation, M2013310, produced an aromatic alcohol with a rose-honey scent; gas chromatography tandem mass spectrometry identified this alcohol as 2-phenylethanol. The concentration of 2-phenylethanol reached 3.8 mg/L, 1.79 g/L, and 3.58 g/L, in M3 (NH 4 + ), M3 (NH 4 + + Phe), and M3 (Phe) culture media, after 72 h of fermentation, respectively. The mRNA expression levels of ARO8 encoding aromatic aminotransferases I and ARO10 encoding phenylpyruvate decarboxylase by M2013310 in M3 (Phe) were the lowest of the three different forms of media tested. These results indicated that M2013310 can synthesize 2-phenylethanol via the Shikimate or Ehrlich pathways and the production of 2-phenylethanol may be significantly improved by the over-expression of these two genes. Our research identified a promising strain of yeast (M2013310) that could be used to improve the production of 2-phenylethanol.

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Section: Methodsmentioning

confidence: 99%

Zygosaccharomyces rouxii, an Aromatic Yeast Isolated From Chili Sauce, Is Able to Biosynthesize 2-Phenylethanol via the Shikimate or Ehrlich Pathways

Dai

Song

et al. 2020

Front. Microbiol.

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“…Unexpectedly, S. cerevisiae F15 Δaro8 Δaro9 and F15 Δaro8 Δaro9 Δaro10 produced more methionol than F15 Δaro8. In the absence of Aro8p and Aro9p, other transaminases may compensate or an alternative pathway may convert methanethiol to methionol, confirmed that the Ehrlich pathway genes differ greatly between lab and wine yeast strains, impacting downstream products such as methionol (Deed, Hou, & Kinzurik, 2019).…”

Section: Resultsmentioning

confidence: 90%

Nitrogen metabolism of branched‐chain alcohols acetates in jujube wine assessed by ¹³ C‐labeling

Xia

Liu

Zhao

et al. 2020

J. Food Process. Preserv.

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Winemakers employ a variety of techniques and tools to produce wines with specific flavor profiles. These volatile metabolites, which are derived from sugar and metabolism of amino acid by yeasts, include esters, carbonyls, volatile fatty acids, sulphur compounds, and higher alcohols. Higher alcohols, also known as fusel alcohols, are quantitatively the largest group of aroma compounds in many alcoholic beverages (Amerine et al., 1980; Lilly, Bauer, & Styger, 2006). They are identified by a strong, pungent smell, and taste and can have a significant effect on the sensorial quality and character of wine and brandy

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“…In the Ehrlich pathway, transamination occurs by enzyme aminotransferases I & II encoded by ARO8 and ARO9 genes whereas in the decarboxylation step, enzyme decarboxylase and pyruvate decarboxylases (encoded by ARO10 ) catalyze the reaction [40]. It was observed that the gene, ARO8 , was the first to be activated in the early growth phase whereas ARO9 and ARO10 genes were activated at the start of the stationary phase.…”

Section: Quorum Sensing In Various Fungal Speciesmentioning

confidence: 99%

Fungal Quorum-Sensing Molecules and Inhibitors with Potential Antifungal Activity: A Review

et al. 2019

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The theory of persisting independent and isolated regarding microorganisms is no longer accepted. To survive and reproduce they have developed several communication platforms within the cells which facilitates them to adapt the surrounding environmental changes. This cell-to-cell communication is termed as quorum sensing; it relies upon the cell density and can stimulate several traits of microbes including biofilm formation, competence, and virulence factors secretion. Initially, this sophisticated mode of communication was discovered in bacteria; later, it was also confirmed in eukaryotes (fungi). As a consequence, many quorum-sensing molecules and inhibitors have been identified and characterized in various fungal species. In this review article, we will primarily focus on fungal quorum-sensing molecules and the production of inhibitors from fungal species with potential applications for combating fungal infections.

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The role of yeastARO8, ARO9andARO10genes in the biosynthesis of 3-(methylthio)-1-propanol fromL-methionine during fermentation in synthetic grape medium

Cited by 28 publications

References 55 publications

Zygosaccharomyces rouxii, an Aromatic Yeast Isolated From Chili Sauce, Is Able to Biosynthesize 2-Phenylethanol via the Shikimate or Ehrlich Pathways

Zygosaccharomyces rouxii, an Aromatic Yeast Isolated From Chili Sauce, Is Able to Biosynthesize 2-Phenylethanol via the Shikimate or Ehrlich Pathways

Nitrogen metabolism of branched‐chain alcohols acetates in jujube wine assessed by ¹³ C‐labeling

Fungal Quorum-Sensing Molecules and Inhibitors with Potential Antifungal Activity: A Review

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The role of yeastARO8, ARO9andARO10genes in the biosynthesis of 3-(methylthio)-1-propanol fromL-methionine during fermentation in synthetic grape medium

Cited by 28 publications

References 55 publications

Zygosaccharomyces rouxii, an Aromatic Yeast Isolated From Chili Sauce, Is Able to Biosynthesize 2-Phenylethanol via the Shikimate or Ehrlich Pathways

Zygosaccharomyces rouxii, an Aromatic Yeast Isolated From Chili Sauce, Is Able to Biosynthesize 2-Phenylethanol via the Shikimate or Ehrlich Pathways

Nitrogen metabolism of branched‐chain alcohols acetates in jujube wine assessed by 13 C‐labeling

Fungal Quorum-Sensing Molecules and Inhibitors with Potential Antifungal Activity: A Review

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Product

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Nitrogen metabolism of branched‐chain alcohols acetates in jujube wine assessed by ¹³ C‐labeling