The role of Yap1p and Skn7p‐mediated oxidative stress response in the defence of Saccharomyces cerevisiae against singlet oxygen

Abstract

The production of the reactive oxygen species superoxide and hydrogen peroxide in Saccharomyces cerevisiae induces the expression of various defence genes involved in an oxidative stress response. Expression of many of these genes has been shown to be coordinated by two transcriptional regulators, Yap1p and Skn7p, either alone or in concert. Here, we investigated the role of the Yap1p and Skn7p-mediated stress response in the defence against singlet oxygen, a non-radical reactive oxygen species produced mainly by photosensitized reactions in illuminated cells. Both, a yap1 and skn7 mutant were highly sensitive to Rose Bengal, an exogenous photosensitizer producing singlet oxygen in the light. The expression of a Yap1p-dependent reporter gene was induced by increased singlet oxygen production, showing that singlet oxygen activates general oxidative stress response mechanisms required for the resistance against Rose Bengal treatment. This response was also slightly stimulated by light in the absence of the photosensitizer, possibly due to singlet oxygen production by endogenous photosensitizers. The expression pattern of four oxidative stress genes in a yap1, skn7 and wild-type strain and the sensitivity of the corresponding mutants exposed to different oxidative stress conditions proved a role of Yap1p and Skn7p in the defence against singlet oxygen. Similarities in the genetic responses against singlet oxygen and hydroperoxides suggest an overlap in the oxidative stress response against these reactive oxygen species.

Nonapoptotic Death of Saccharomyces cerevisiae Cells That Is Stimulated by Hsp90 and Inhibited by Calcineurin and Cmk2 in Response to Endoplasmic Reticulum Stresses

“…The stress-responsive transcription factors Yap1 and Skn7 have also been reported as targets of calcineurin in other conditions (8). The yap1 skn7 double mutant also retained full responsiveness to FK506, Crz1 loss, and Cmk2 loss (Fig.…”

Deciphering Dynamic Dose Responses of Natural Promoters and Single cis Elements upon Osmotic and Oxidative Stress in Yeast

“…Two main transcriptional activators, Yap1 and Skn7, have been implicated in this adaptive response (29)(30)(31). Although many oxidative stress-responsive defense genes are regulated by both factors, it seems that Yap1 and Skn7 respond to different oxidative stimuli (32,33). The Yap1 basic leucine zipper protein preferentially binds to AP-1 sequences (5=-TTACTAA-3=) in promoters of antioxidant genes (34).…”

Signalling Oxidative Stress in Saccharomyces cerevisiae

“…Msn2/Msn4 nuclear localisation and activity are regulated by both TORC1 and PKA (detailed formerly). For the induction of many antioxidant genes, Skn7 and Yap1 act cooperatively upon oxidative stress (Lee et al, 1999;Brombacher et al, 2006;He et al, 2005). The contribution of Skn7 to the oxidative stress response does not occur through any of the cysteines of the protein.…”