The Role of Xenopus Membrane Progesterone Receptor β in Mediating the Effect of Progesterone on Oocyte Maturation

Ben‐Yehoshua, Liat Josefsberg; Lewellyn, Andrea L.; Thomas, Peter; Maller, James L.

doi:10.1210/me.2006-0256

Cited by 99 publications

(54 citation statements)

References 52 publications

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“…The details of this cascade are best defined in Xenopus oocytes with close parallels in mammals. Progesterone treatment results in a decrease in cAMP levels in Xenopus oocytes through the activation of a progesterone cell surface serpentine G-protein coupled receptor [86;87]. This induces poly-adenylation of oocyte mRNAs leading to accumulation of the oocyte specific MAP kinase kinase, Mos [88].…”

Section: Introductionmentioning

confidence: 99%

Ca2+ signaling, genes and the cell cycle

Machaca

2010

Cell Calcium

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Changes in the concentration and spatial distribution of Ca2+ ions in the cytoplasm constitute a ubiquitous intracellular signaling module in cellular physiology. With the advent of Ca2+ dyes that allow direct visualization of Ca2+ transients, combined with powerful experimental tools such as electrophysiological recordings, intracellular Ca2+ transients have been implicated in practically every aspect of cellular physiology, including cellular proliferation. Ca2+ signals are associated with different phases of the cell cycle and interfering with Ca2+ signaling or downstream pathways often disrupts progression of the cell cycle. Although there exists a dependence between Ca2+ signals and the cell cycle the mechanisms involved are not well defined and given the cross-talk between Ca2+ and other signaling modules, it is difficult to assess the exact role of Ca2+ signals in cell cycle progression. Two exceptions however, include fertilization and T-cell activation, where well-defined roles for Ca2+ signals in mediating progression through specific stages of the cell cycle have been clearly established. In the case of T-cell activation Ca2+ regulates entry into the cell cycle through the induction of gene transcription.

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Section: Introductionmentioning

confidence: 99%

Ca2+ signaling, genes and the cell cycle

Machaca

2010

Cell Calcium

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“…However, progesterone has also been shown to act on receptors intracellularly to induce rapid signaling cascades inside the cell [12,22,66,231]. Despite extensive research on oocyte maturation in amphibians, considerable controversy surrounds the identity of the steroid receptor mediating this nongenomic steroid action, and nuclear progestin receptor (PR) or PR-like receptors [12,231], the androgen receptor (AR) [120], as well as a novel membrane progestin receptor, mPRβ [97], have been proposed as intermediaries. The PR has been clearly implicated in the activation of intracellular second messengers in a variety of cells, including breast cancer cells [22,193].…”

Section: Nonclassical Progestin Actions and Progestin Receptorsmentioning

confidence: 99%

“…The mPRs belong to the progestin and adipoQ receptor (PAQR) vertebrate family of 7-TM proteins, with no structural or sequence homology to nuclear steroid receptors, or GPCRs [206,221]. Specific progestin receptor binding has been demonstrated with recombinant vertebrate mPRα (PAQR7), mPRβ (PAQR8), mPRγ (PAQR5) and two additional members of the PAQR family, mPRδ (PAQR6) and mPRε (PAQR9) produced in prokaryotic ( E.coli ), mammalian, and yeast expression systems by several research groups [6,7,82,97,196,221,233,260,262]. The recombinant mPRs expressed in mammalian expression systems display high affinity binding (K d 3–7 nM).…”

Section: Nonclassical Progestin Actions and Progestin Receptorsmentioning

confidence: 99%

Rapid steroid hormone actions initiated at the cell surface and the receptors that mediate them with an emphasis on recent progress in fish models

Thomas

2012

General and Comparative Endocrinology

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143

105

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In addition to the classic genomic mechanism of steroid action mediated by activation of intracellular nuclear receptors, there is now extensive evidence that steroids also activate receptors on the cell surface to initiate rapid intracellular signaling and biological responses that are often nongenomic. Recent progress in our understanding of rapid, cell surface-initiated actions of estrogens, progestins, androgens and corticosteroids and the identities of the membrane receptors that act as their intermediaries is briefly reviewed with a special emphasis on studies in teleost fish. Two recently discovered novel proteins with seven-transmembrane domains, G protein-coupled receptor 30 (GPR30), and membrane progestin receptors (mPRs) have the ligand binding and signaling characteristics of estrogen and progestin membrane receptors, respectively, but their functional significance is disputed by some researchers. GPR30 is expressed on the cell surface of fish oocytes and mediates estrogen inhibition of oocyte maturation. mPRα is also expressed on the oocyte cell surface and is the intermediary in progestin induction of oocyte maturation in fish. Recent results suggest there is cross-talk between these two hormonal pathways and that there is reciprocal down-regulation of GPR30 and mPRα expression by estrogens and progestins at different phases of oocyte development to regulate the onset of oocyte maturation. There is also evidence in fish that mPRs are involved in progestin induction of sperm hypermotility and anti-apoptotic actions in ovarian follicle cells. Nonclassical androgen and corticosteroid actions have also been described in fish models but the membrane receptors mediating these actions have not been identified.

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“…However, seasonal differences in the sensitivity of frog oocytes to induction of meiosis by progesterone and ceramide as well as different effects of them on membrane microdomain integrity in toad oocytes have been observed [79, 81, 82]. Moreover, the importance of mPRs in mediating the non-genomic action of progesterone to induce of oocyte meiotic maturation in amphibians remains unclear because nuclear PRs or PR-like forms have also been implicated [31, 83, 84]. In contrast, extensive evidence has been obtained that mPRs are the primary intermediaries in progestin induction of oocyte maturation in teleosts [5].…”

Section: Potential Role Of Mprs In Sphingolipid Signalingmentioning

confidence: 99%

Membrane Progesterone Receptors: Evidence for Neuroprotective, Neurosteroid Signaling and Neuroendocrine Functions in Neuronal Cells

Thomas¹,

Pang²

2012

Neuroendocrinology

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142

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Membrane progesterone receptors (mPRs) are novel G protein-coupled receptors belonging to the progestin and adipoQ receptor family (PAQR) that mediate a variety of rapid cell surface-initiated progesterone actions in the reproductive system involving activation of intracellular signaling pathways (i.e. nonclassical actions). The mPRs are highly expressed in the brain, but research on their neural functions has only been conducted in a single neuronal cell line, GT1–7 cells, which have negligible nuclear progesterone receptor (PR) expression. GT1–7 cells express mPRα and mPRβ on their plasma membranes which is associated with the presence of high-affinity, specific [³H]-progesterone receptor binding. The neurosteroid, allopregnanolone, is an effective ligand for recombinant mPRα with a relative binding affinity of 7.6% that of progesterone. Allopregnanolone acts as a potent mPR agonist on GT1–7 cells, mimicking the progesterone-induced decrease in cAMP accumulation and its antiapoptotic actions at low nanomolar concentrations. The decrease in cAMP levels is associated with rapid progesterone-induced downregulation of GnRH pulsatile secretion from perifused GT1–7 cells. The recent suggestion that mPRs are alkaline ceramidases and mediate sphingolipid signaling is not supported by empirical evidence that TNFα does not bind to mPRs overexpressed in human cells and that exogenous sphingomyelinase is ineffective in mimicking progestin actions through mPRs to induce meiotic maturation of fish oocytes. Taken together, these recent studies indicate that mPRs mediate neuroprotective effects of progesterone and allopregnanolone and are also the likely intermediaries in progesterone-induced inhibition of pulsatile GnRH secretion in GT1–7 cells.

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The Role of Xenopus Membrane Progesterone Receptor β in Mediating the Effect of Progesterone on Oocyte Maturation

Cited by 99 publications

References 52 publications

Ca2+ signaling, genes and the cell cycle

Ca2+ signaling, genes and the cell cycle

Rapid steroid hormone actions initiated at the cell surface and the receptors that mediate them with an emphasis on recent progress in fish models

Membrane Progesterone Receptors: Evidence for Neuroprotective, Neurosteroid Signaling and Neuroendocrine Functions in Neuronal Cells

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