The role of Wnt signaling in male reproductive physiology and pathology

Xue, Ruizhi; Lin, Wenfeng; Sun, Jiaqian; Watanabe, Masami; Xu, Aiming; Araki, Motoo; Nasu, Yasutomo; Huang, Peng

doi:10.1093/molehr/gaaa085

Cited by 13 publications

(10 citation statements)

References 94 publications

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“…No differences in the expression levels of the gene coding for the core effector of the pathway, β-catenin (e.g. [ 25 , 40 , 42 , 57 ]), were observed either (see Fig 5A ).…”

Section: Resultsmentioning

confidence: 98%

“…We considered this an attractive idea, as accumulating evidence indicates the relevance of Wnt signaling in testis development and physiology, and its alteration in different male reproductive anomalies (e.g. [ 40 , 42 , 66 ]). However, when comparing Spats1 KO vs WT mice, we could not find any significant change in the expression of common Wnt pathway-target genes in the testis, which is the tissue where Spats1 is mostly expressed.…”

Section: Discussionmentioning

confidence: 99%

“…As spermatogenesis requires a fine-tuning of the Wnt pathway (e.g. [40][41][42]), we wondered if this pathway would be disregulated in the testes of Spats1 -/mice. In order to answer this question, we analyzed the expression levels of some of the most common Wnt pathway-target genes that are activated by the β-catenin/TCF4 complex, namely Lef1, Tcf1, Cyclin D1 (Ccnd1), and c-Myc [39,41,[43][44][45], in the testes of Spats1 -/mice, in comparison to WT ones.…”

Section: No Differences In the Expression Level Of Genes From The Wnt Pathway Are Detected Between The Testes Of Spats1 -/And Wt Micementioning

confidence: 99%

“…Wnt4, one of the initiators of Wnt signaling, is a key gene for the development of female genitalia, with its absence leading to partial female-to-male sex reversal in mouse (e.g. [42,49]). A fine tuning of Wnt4 expression is also implicated in testis development and spermatogenesis [50][51][52][53][54][55].…”

Section: No Differences In the Expression Level Of Genes From The Wnt Pathway Are Detected Between The Testes Of Spats1 -/And Wt Micementioning

confidence: 99%

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SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse

et al. 2021

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SPATS1 (spermatogenesis-associated, serine-rich 1) is an evolutionarily conserved, testis-specific protein that is differentially expressed during rat male meiotic prophase. Some reports have suggested a link between SPATS1 underexpression/mutation and human pathologies such as male infertility and testicular cancer. Given the absence of functional studies, we generated a Spats1 loss-of-function mouse model using CRISPR/Cas9 technology. The phenotypic analysis showed no overt phenotype in Spats1-/- mice, with both males and females being fertile. Flow cytometry and histological analyses did not show differences in the testicular content and histology between WT and knockout mice. Moreover, no significant differences in sperm concentration, motility, and morphology, were observed between WT and KO mice. These results were obtained both for young adults and for aged animals. Besides, although an involvement of SPATS1 in the Wnt signaling pathway has been suggested, we did not detect changes in the expression levels of typical Wnt pathway-target genes in mutant individuals. Thus, albeit Spats1 alteration might be a risk factor for male testicular health, we hereby show that this gene is not individually essential for male fertility and spermatogenesis in mouse.

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“…No differences in the expression levels of the gene coding for the core effector of the pathway, β-catenin (e.g. [ 25 , 40 , 42 , 57 ]), were observed either (see Fig 5A ).…”

Section: Resultsmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: No Differences In the Expression Level Of Genes From The Wnt Pathway Are Detected Between The Testes Of Spats1 -/And Wt Micementioning

confidence: 99%

Section: No Differences In the Expression Level Of Genes From The Wnt Pathway Are Detected Between The Testes Of Spats1 -/And Wt Micementioning

confidence: 99%

See 2 more Smart Citations

SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse

et al. 2021

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“…To better understand the molecular regulatory basis of the Es-RPCH and Es-β-PDH in ovarian maturation, we next performed ovarian transcriptome analysis. KEGG pathway analysis showed that reproduction-related pathways such as the Wnt and TGF‐β signaling pathway were enriched ( 45 , 46 ) ( Figure S3 ). Interestingly, some key genes involved in meiosis and ovarian development, such as cyclin B, Cdc2, 5-HT-R, RXR and FOXL2, were detected to be upregulated in Es-RPCH group ( Figure 8 ).…”

Section: Discussionmentioning

confidence: 99%

Evidences for Red Pigment Concentrating Hormone (RPCH) and Beta-Pigment Dispersing Hormone (β-PDH) Inducing Oocyte Meiotic Maturation in the Chinese Mitten Crab, Eriocheir sinensis

et al. 2021

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Red pigment concentrating hormone (RPCH) and pigment dispersing hormone (PDH) are crustacean neuropeptides involved in broad physiological processes including body color changes, circadian rhythm, and ovarian growth. In this study, the full-length cDNA of RPCH and PDH were identified from the brain of the Chinese mitten crab Eriocheir sinensis. The deduced RPCH and PDH mature peptides shared identical sequence to the adipokinetic hormone/RPCH peptides family and the β-PDH isoforms and were designated as Es-RPCH and Es-β-PDH, respectively. Es-RPCH and Es-β-PDH transcripts were distributed in the brain and eyestalks. The positive signals of Es-RPCH and Es-β-PDH were localized in the neuronal clusters 6, 8, 9, 10, and 17 of the brain as revealed by in situ hybridization. The expression level of Es-RPCH and Es-β-PDH mRNA in nervous tissues were all significantly increased at vitellogenic stage, and then decreased at the final meiotic maturation stage. The administrated with synthesized Es-RPCH peptide results in germinal vesicles shift toward the plasma membrane in vitellogenic oocyte, and significant decrease of the gonad-somatic index (GSI) and mean oocyte diameter as well as the expression of vitellogenin mRNA at 30 days post injection in vivo. Similar results were also found when injection of the Es-β-PDH peptide. In vitro culture demonstrated that Es-RPCH and Es-β-PDH induced germinal vesicle breakdown of the late vitellogenic oocytes. Comparative ovarian transcriptome analysis indicated that some reproduction/meiosis-related genes such as cdc2 kinase, cyclin B, 5-HT-R and retinoid-X receptor were significantly upregulated in response to Es-RPCH and Es-β-PDH treatments. Taken together, these results provided the evidence for the inductive effect of Es-RPCH and Es-β-PDH on the oocyte meiotic maturation in E. sinensis.

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Di-(2-ethylhexyl) phthalate exacerbates abnormalities of testicular development in F1 males via inhibition the Wnt/β-catenin signaling pathway

Li,

Sun,

Chang

et al. 2024

Environmental Pollution

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The role of Wnt signaling in male reproductive physiology and pathology

Cited by 13 publications

References 94 publications

SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse

SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse

Evidences for Red Pigment Concentrating Hormone (RPCH) and Beta-Pigment Dispersing Hormone (β-PDH) Inducing Oocyte Meiotic Maturation in the Chinese Mitten Crab, Eriocheir sinensis

Di-(2-ethylhexyl) phthalate exacerbates abnormalities of testicular development in F1 males via inhibition the Wnt/β-catenin signaling pathway

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