The role of water in the activation ofPhycomyces blakesleeanus sporangiospores

Verbeke, Marc N.; Laere, André J. Van

doi:10.1016/0147-5975(86)90003-4

Cited by 2 publications

(2 citation statements)

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“…2) and to decrease in density (Van Laere et al, 1 9 8 0~) during the first 30 min of germination. A loosening of the cell wall might also be involved in this process (Verbeke & Van Laere, 1982, 1986, but it remains difficult to distinguish between cause and effect in this respect.…”

Section: Role Of Trehalose Breakdownmentioning

confidence: 99%

“…Although rehydration of the spores after suspension in water occurs in a split second, the water content of spores hydrated in water-saturated air has been reported to be as low as 50% (Verbeke & Van Laere, 1986) to 60% (Furch, 1978). With such low water contents, the availability and/or mobility of water might limit enzymic activity in the spores, especially if the substrates are large molecules (Potthast, 1978).…”

Section: Introductionmentioning

confidence: 99%

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Relation between Germination, Trehalose and the Status of Water in Phycomyces blakesleeanus Spores as Measured by Proton-NMR

et al. 1987

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The distribution and properties of the cellular water in sporangiospores of Phycomyces blakesleeanus were investigated using proton-NMR. In dormant spores different classes of water were characterized by a difference in their transverse relaxation times (T2). The amount of cytoplasmic water was estimated to be as low as about 700 mg (g dry wt)-' and its small T2 (1 8.2 ms) indicated a very limited mobility. About 10 min after induction of germination (by a heat shock or by addition of 0.1 M-acetate), both the content and the mobility of the cytoplasmic water increased sharply. These changes coincided with a rapid breakdown of most of the cellular trehalose and with the production (and leakage from the spores) of large amounts of glycerol. The role of these biochemical changes is discussed in relation to the water status of the spores.

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Section: Role Of Trehalose Breakdownmentioning

confidence: 99%