The role of Vibrio vulnificus virulence factors and regulators in its infection-induced sepsis

Li, Gang; Wang, Mingyi

doi:10.1007/s12223-019-00763-7

Cited by 29 publications

(12 citation statements)

References 107 publications

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“…Vibrio (V.) vulnificus is a pathogenic marine bacterium associated with foodborne illnesses, often causing gastroenteritis, septicemia, and diarrhea [1]. Infection with V. vulnificus is cytotoxic to host cells, and its virulence is mediated by secreted cytotoxins and enzymes, such as VvhA, MARTX, VvpE, and VvpM [1][2][3][4][5][6]. VvhA is one of the most potent pore-forming cytotoxins capable of killing mice at sub-µg levels, and it plays a crucial role in the pathogenesis and dissemination of V. vulnificus by facilitating intestinal paracellular permeabilization [2].…”

Section: Introductionmentioning

confidence: 99%

Nanosphere Loaded With Curcumin Inhibits the Gastrointestinal Cell Death Signaling Pathway Induced by the Foodborne Pathogen Vibrio vulnificus

Kim

Lee

Kim

et al. 2020

Cells

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Curcumin, a hydrophobic polyphenol of turmeric, has a variety of biological functions as a herbal supplement, but its poor gastric absorption rate is one of the major factors limiting its oral bioavailability. In the present study, we have investigated the functional role of a nanosphere loaded with curcumin (CN) during host cell death elicited by the Gram-negative bacterium V. vulnificus in human gastrointestinal epithelial HT-29 cells and an ileal-ligated mouse model. The recombinant protein (r) VvhA produced by V. vulnificus significantly reduced the viability of HT-29 cells. The cytotoxic effect of rVvhA was restored upon a treatment with CN (100 ng/mL), which had shown 1000-fold higher anti-apoptotic efficacy than curcumin. CN inhibited the phosphorylation of c-Src and PKC mediated by intracellular ROS responsible for the distinctive activation of the MAPKs in rVvhA-treated HT-29 cells. Interestingly, CN significantly restored the expression of Bax, Bcl-2, and cleaved caspase-3 as regulated by the phosphorylation of NF-κB. In mouse models of V. vulnificus infection, treatment with CN had a blocking effect that elevated the levels of TUNEL-positive DNA fragmentation and apoptosis-related proteins. These results indicate that CN is a functional agent that manipulates the V. vulnificus VvhA signaling pathway responsible for gastrointestinal cell death.

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Section: Introductionmentioning

confidence: 99%

Nanosphere Loaded With Curcumin Inhibits the Gastrointestinal Cell Death Signaling Pathway Induced by the Foodborne Pathogen Vibrio vulnificus

Kim

Lee

Kim

et al. 2020

Cells

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“…Vibrio vulnificus is a gram-negative, opportunistic bacillus responsible of wound infections, gastroenteritis, and septicemia. Inhabits saltwater flora and fauna, multiple cases have been reported annually [3] , [4] in regions with a subtropical climate [5] ; however, in recent years significant increases in expansion of the geographic area have been observed in infections caused by this pathogen, probably due to global warming increasing ocean temperature [5] , [6] .…”

Section: Discussionmentioning

confidence: 99%

“…Biotype 1 strains are responsible of most infections in humans, while biotype 2 strains (non-pathogenic for humans) are responsible for infections in marine eels. The strains of biotype 3, which cause fewer skin infections in humans, have been limited to people who handle fish [6] , [7] .…”

Section: Discussionmentioning

confidence: 99%

Purpura fulminans due to Vibrio vulnificus severe infection

Guadarrama

Eseberre

Vazquez

et al. 2022

IDCases

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“…Symptoms following consumption of infected seafood include vomiting, diarrhea, abdominal pain, and blistering dermatitis [ 10 ]. The less common infection via wounds often takes a serious course to sepsis with a rapidly fulminant progression to septic shock [ 11 ]. Patients with chronic diseases, weakened immune system, and open wounds are particularly at risk.…”

Section: Introductionmentioning

confidence: 99%

“The Baltic Sea Germ”: A Case Report of Necrotizing Fasciitis following Vibrio vulnificus Infection

Meyer

Polan

Burggraf

et al. 2022

Case Reports in Orthopedics

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Reported is an 80-year-old patient with septic shock from necrotizing fasciitis secondary to a Vibrio vulnificus (Vv) infection. The patient reports having been swimming in the Baltic Sea after a minor trauma to the left leg. Emergency superficial necrosectomy followed by intensive medical therapy was performed. Antibiotic therapy was initiated with a third-generation cephalosporin and a tetracycline. Vv was detected in the intraoperative microbiological smears. Instead of a leg amputation and a flap, due to the patient’s age, a split skin covering to consolidate the wound was performed. Vv is a gram-negative rod bacterium of the genus Vibrio. Vv occurs in warm, low-salinity seawater (brackish water). In Germany, Vv occurs primarily in river mouths of the low-salinity Baltic Sea. Infections by Vv can occur through open wounds or by eating raw infected seafood, especially oysters. Infection via wounds often take a fulminant lethal course. Patients with chronic diseases, weakened immune system, and open wounds are particularly at risk. Infections with Vv are rare, but occur worldwide. Global warming is expected to spread Vv as water temperature increases and the dilution effect of sea level rise further decreases ocean salinity, and natural disasters promote the spread of Vv.

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The role of Vibrio vulnificus virulence factors and regulators in its infection-induced sepsis

Cited by 29 publications

References 107 publications

Nanosphere Loaded With Curcumin Inhibits the Gastrointestinal Cell Death Signaling Pathway Induced by the Foodborne Pathogen Vibrio vulnificus

Nanosphere Loaded With Curcumin Inhibits the Gastrointestinal Cell Death Signaling Pathway Induced by the Foodborne Pathogen Vibrio vulnificus

Purpura fulminans due to Vibrio vulnificus severe infection

“The Baltic Sea Germ”: A Case Report of Necrotizing Fasciitis following Vibrio vulnificus Infection

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