The role of vasodilator‐stimulated phosphoprotein in podocyte functioning

Rachubik, Patrycja; Piwkowska, Agnieszka

doi:10.1002/cbin.11149

Cited by 6 publications

(8 citation statements)

References 94 publications

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“…Knockdown of VASP downregulates the expression of osteoclast-specific genes. VASP has been reported to play an important role in cell behaviour, including in cell differentiation (1,2). To further elucidate the role of VASP in osteoclast differentiation, VASP expression was knocked down in RAW 264.7 cells using si-RNA post-RANKL treatment.…”

Section: Rankl Treatment Increases Osteoclast Differentiationmentioning

confidence: 99%

“…Osteoclast function is important in clinical disorders of bone resorption, such as osteoarthritis, rheumatoid arthritis and osteoporosis. Vasodilator-stimulated phosphoprotein (VASP) has been demonstrated to be involved in several cell processes, including differentiation and mobility (1). VASP has been reported to serve an important role in cell migration and tumour metastasis (1).…”

Section: Introductionmentioning

confidence: 99%

“…Vasodilator-stimulated phosphoprotein (VASP) has been demonstrated to be involved in several cell processes, including differentiation and mobility (1). VASP has been reported to serve an important role in cell migration and tumour metastasis (1). Previously, we demonstrated VASP protein regulates osteosarcoma cell migration and metastasis, potentially through interactions with Ras-related C3 botulinum toxin substrate 1 (Rac1) (2).…”

Section: Introductionmentioning

confidence: 99%

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Role of vasodilator‑stimulated phosphoprotein in RANKL‑differentiated murine macrophage RAW264.7 cells: Modulation of NF‑κB, c‑Fos and NFATc1 transcription factors

Zhang

et al. 2021

Exp Ther Med

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Vasodilator-stimulated phosphoprotein (VASP) is essential for osteoclast differentiation, and reduced VASP expression results in depressed osteoclast differentiation. Previously, we demonstrated the importance of VASP and Ras-related C3 botulinum toxin substrate 1 interactions in osteosarcoma cell migration and metastasis using Mg-63 and Saos2 cells. However, the molecular details of the functional role of VASP in cell motility and migration remain to be elucidated. The present study demonstrated that VASP affects the expression of αV-integrin, tartrate-resistant acid phosphatase (TRAP) and lamellipodia protrusion in RAW 264.7 murine macrophage cells. The RAW 264.7 mouse monocyte macrophage cell line was used as an osteoclast precursor. RAW 264.7 cells were treated with 50 ng/ml of receptor activator of nuclear factor κ-Β ligand (RANKL) in order to induce cell differentiation (osteoclastogenesis). Small interfering RNA (siRNA) was used to silence VASP, and RT-PCR and western blotting were used to determine the expression for genes and proteins, respectively. TRAP staining as a histochemical marker for osteoclast and fluorescent microscopy for lamellipodia protrusion was performed. RANKL treatment significantly increased the gene and protein expression of VASP, αV-integrin and TRAP in RAW 264.7 cells. Silencing of VASP significantly reduced the RANKL-induced expression of αV-integrin, TRAP and lamellipodia protrusion. In addition, knockdown of VASP attenuated RANKL-stimulated activation of NF-κB, c-Fos and nuclear factor of activated T cells cytoplasmic 1 transcription factors, and the phosphorylation of the p65 and IκBα. These results suggest the critical role of VASP in regulating osteoclast differentiation, which should be further explored in osteosarcoma research.

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Section: Rankl Treatment Increases Osteoclast Differentiationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Role of vasodilator‑stimulated phosphoprotein in RANKL‑differentiated murine macrophage RAW264.7 cells: Modulation of NF‑κB, c‑Fos and NFATc1 transcription factors

Zhang

et al. 2021

Exp Ther Med

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“…Interestingly, VASP is highly expressed in lung adenocarcinoma tissues. It correlates strongly with tumor differentiation, lymph node metastasis and invasion, and survival and prognosis of patients [ 4 – 7 ]. The inconsistency on the relationship between the VASP level and the occurrence of tumors warrants further research.…”

Section: Introductionmentioning

confidence: 99%

Clinical Significance of Detection of Peripheral Blood VASP Level in Lung Cancer Patients

Zhu

Chen

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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This paper explores the relationship between the clinical value of vasodilator-stimulated phosphoprotein (VASP) in lung cancer tissue and its diagnosis and severity. Totally 100 patients who were clinically diagnosed with lung cancer from January 2018 to December 2020 were enrolled in our study. They were assigned into two groups according to the presence of lymph node metastasis. The VASP levels were measured by flow cytometry. The correlation between the expression of VASP in tumor tissue and the clinical characteristics and prognosis in patients was analyzed. The diagnostic efficacy of plasma VASP with squamous cell carcinoma antigen (SCC), neuron-specific enolase (NSE), cytokeratin-19 fragment (CYFRA21-1), prosecretin-releasing peptide (proGRP), and lung cancer was analyzed. The results were compared with APACHE III score to evaluate the accuracy of VASP in determining the severity of patients. This paper finds that the value of VASP in the non-lymph node metastasis group was significantly higher than that in the healthy control group, and the VASP level in the lymph node metastasis group was significantly higher than that in the non-lymph node metastasis group and the healthy control group (all p values <0.05). The APACHE III score of the lymph node metastasis group was higher than that of the non-lymph node metastasis group ( p value <0.05). The diagnostic efficacy of VASP is similar to that of SCC, NSE, CYFRA21-1, and proGRP. The plasma VASP value was statistically different in the survival group and the death group, with higher level observed in the death group compared to survival group (all p values <0.05). The value of plasma VASP alone and acute physiology and chronic health evaluations III (APACHE III) score for lung cancer mortality was similar (47.06% vs. 52.94%, p value >0.05). Similar accuracy was observed in VASP and APACHE III score in predicting mortality of lung cancer (84.37% vs. 85.77%, p value>0.05). This paper concludes that the level of VASP correlates to the severity of the lung cancer and survival of the patients.

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“…They consist of three significant structural elements: a cell body, major processes, and foot processes. Adjacent foot processes of podocytes entwine glomerular capillaries, forming a slit diaphragm (Coward & Fornoni, 2015; Garg, 2018; Pollak et al, 2014; Rachubik & Piwkowska, 2019; Scott & Quaggin, 2015). The slit diaphragm is the most important part of the glomerular filtration barrier.…”

Section: Introductionmentioning

confidence: 99%

The impact of type III sodium‐dependent phosphate transporters (Pit 1 and Pit 2) on podocyte and kidney function

Kulesza

Piwkowska

2021

Journal Cellular Physiology

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The sodium‐dependent phosphate transporters Pit 1 and Pit 2 belong to the solute carrier 20 (SLC20) family of membrane proteins. They are ubiquitously distributed in the human body. Their crucial function is the intracellular transport of inorganic phosphate (Pi) in the form of H2PO4 −. They are one of the main elements in maintaining physiological phosphate homeostasis. Recent data have emerged that indicate novel roles of Pit 1 and Pit 2 proteins besides the well‐known function of Pi transporters. These membrane proteins are believed to be precise phosphate sensors that mediate Pi‐dependent intracellular signaling. They are also involved in insulin signaling and influence cellular insulin sensitivity. In diseases that are associated with hyperphosphatemia, such as diabetes and chronic kidney disease (CKD), disturbances in the function of Pit 1 and Pit 2 are observed. Phosphate transporters from the SLC20 family participate in the calcification of soft tissues, mainly blood vessels, during the course of CKD. The glomerulus and podocytes therein can also be a target of pathological calcification that damages these structures. A few studies have demonstrated the development of Pi‐dependent podocyte injury that is mediated by Pit 1 and Pit 2. This paper discusses the role of Pit 1 and Pit 2 proteins in podocyte function, mainly in the context of the development of pathological calcification that disrupts permeability of the renal filtration barrier. We also describe the mechanisms that may contribute to podocyte damage by Pit 1 and Pit 2.

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The role of vasodilator‐stimulated phosphoprotein in podocyte functioning

Cited by 6 publications

References 94 publications

Role of vasodilator‑stimulated phosphoprotein in RANKL‑differentiated murine macrophage RAW264.7 cells: Modulation of NF‑κB, c‑Fos and NFATc1 transcription factors

Role of vasodilator‑stimulated phosphoprotein in RANKL‑differentiated murine macrophage RAW264.7 cells: Modulation of NF‑κB, c‑Fos and NFATc1 transcription factors

Clinical Significance of Detection of Peripheral Blood VASP Level in Lung Cancer Patients

The impact of type III sodium‐dependent phosphate transporters (Pit 1 and Pit 2) on podocyte and kidney function

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