The role of vascular endothelial growth factor in ossification

Yang, Yanqi; Tan, Yingying; Wong, Ricky W.K.; Wenden, Alex; Zhang, Lin-Kun; Rabie, A. Bakr M.

doi:10.1038/ijos.2012.33

Cited by 235 publications

(193 citation statements)

References 51 publications

(66 reference statements)

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“…Endothelial cell growth is regulated by vascular endothelial growth factor (VEGF), which also acts as an essential mediator during endochondrial ossification and intramembranous ossification. VEGF is involved in many processes, including osteoblast differentiation and recruitment of osteoclasts, and previous evidence suggests joint contribution of angiogenesis and bone formation during skeletal development 34, 35…”

Section: Discussionmentioning

confidence: 99%

Bone Mineral Density Is Positively Related to Carotid Intima-Media Thickness: Findings From a Population-Based Study in Adolescents and Premenopausal Women

Frysz

Deere

Lawlor

et al. 2016

Journal of Bone and Mineral Research

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Osteoporosis and cardiovascular disease (CVD) are both common causes of morbidity and mortality. Previous studies, mainly of people older than 60 years, suggest a relationship between these conditions. Our aim was to determine the association between bone characteristics and CVD markers in younger and middle‐aged individuals. Women (n = 3366) and their adolescent offspring (n = 4368) from the UK population‐based cohort study, Avon Longitudinal Study of Parents and Children (ALSPAC), were investigated. We measured total body (TB) and hip bone mineral density (BMD), TB bone area (BA) and bone mineral content (BMC) by dual‐energy X‐ray absorptiometry (DXA), and carotid intima‐media thickness (cIMT) by high‐resolution ultrasound. Arterial distensibility was calculated as the difference between systolic and diastolic arterial diameters. Linear regression determined associations between bone exposures and cIMT (in adolescents) and both cIMT and arterial distensibility (in women), generating partial correlation coefficients. Mean (SD) age of women was 48 (4.2) years, body mass index (BMI) was 26.2 (5.0) kg/m2, and 71% were premenopausal. In confounder‐adjusted analyses (age, height, lean mass, fat mass, menopause, smoking, estrogen replacement, calcium/vitamin D supplementation, and education) TB and hip BMD were both positively associated with cIMT (0.071 [0.030, 0.112], p = 0.001; 0.063 [0.025, 0.101], p = 0.001, respectively). Femoral neck BMD and TB BMD, BMC, and BA were positively associated with arterial distensibility. Mean (SD) age of adolescents was 17 (0.4) years, BMI was 23 (4.1) kg/m2, and 44.5% were male. Total hip and TB measurements were positively associated with cIMT, with similar magnitudes of association to those found in their mothers. In contrast to most published findings, we identified weak positive associations between BMD and cIMT in predominantly premenopausal women and their adolescent offspring. We found greater femoral neck BMD and TB DXA measurements to be associated with reduced arterial stiffness. Rather than a relationship with preclinical atherosclerosis, in these relatively young populations, we speculate our associations between BMD, cIMT, and arterial distensibility may reflect a shared relationship between bone and vascular growth and development. © 2016 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research.

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Section: Discussionmentioning

confidence: 99%

Bone Mineral Density Is Positively Related to Carotid Intima-Media Thickness: Findings From a Population-Based Study in Adolescents and Premenopausal Women

Frysz

Deere

Lawlor

et al. 2016

Journal of Bone and Mineral Research

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“…In vitro analysis from donor 1 showed a decrease in both VEGF and COLX gene expression compared to the other two donors. VEGF is known to be involved in vascularisation (Yang et al, 2012), which means that this decreased expression of VEGF could be attributed to the lack of bone marrow elements we observed following in vivo implantation. Additionally, donor 1 showed greater COLII expression and GAG accumulation, as well as, decreased COLX gene expression compared to donors 2 and 3.…”

Section: Ca Knuth Et Almentioning

confidence: 96%

Mesenchymal stem cell-mediated endochondral ossification utilising micropellets and brief chondrogenic priming

Knuth¹,

Witte-Bouma²,

Ridwan³

et al. 2017

eCM

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With limited autologous and donor bone graft availability, there is an increasing need for alternative graft substitutes. We have previously shown that chondrogenically priming mesenchymal stem cell (MSC) pellets for 28 d in vitro will reproducibly result in endochondral bone formation after in vivo implantation. However, pellet priming time for clinical applications is quite extensive. A micropellet (μpellet)-fibrin construct was developed and coupled, with a shorter priming period, determined by an in vitro time course experiment. In vitro data showed expression of chondrogenic genes and matrix production after 7 d of chondrogenic priming, indicating that briefer priming could possibly be used to induce bone formation in vivo. 7 and 28 d primed pellet, pellet-fibrin and μpellet-fibrin constructs were cultured for in vitro analysis and implanted subcutaneously for 8 weeks into nude mice. μpellet-fibrin constructs, cultured in vitro for 7 or 28 d, produced comparable bone to standard pellets in vivo. MSC-mediated bone formation was achieved following only 7 d of in vitro priming. Bone formation in vivo appeared to be influenced by overall matrix production pre-implantation. Given this short priming time and the injectable nature of the μpellet-fibrin constructs, this approach might be further developed as an injectable bone substitute, leading to a minimally-invasive treatment option, which would allow for tailored filling of bone defects.

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“…26 Based on these data it could be concluded that the small-sized bone substitute granules might also induce a higher or faster level of bone tissue growth, as VEGF also stimulates the proliferation and differentiation of osteoblasts in a dose-dependent manner. 51,52 However, further studies have to be conducted using also bone implantation models to clarify this issue.…”

mentioning

confidence: 99%

Small-sized granules of biphasic bone substitutes support fast implant bed vascularization

et al. 2015

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The role of vascular endothelial growth factor in ossification

Cited by 235 publications

References 51 publications

Bone Mineral Density Is Positively Related to Carotid Intima-Media Thickness: Findings From a Population-Based Study in Adolescents and Premenopausal Women

Bone Mineral Density Is Positively Related to Carotid Intima-Media Thickness: Findings From a Population-Based Study in Adolescents and Premenopausal Women

Mesenchymal stem cell-mediated endochondral ossification utilising micropellets and brief chondrogenic priming

Small-sized granules of biphasic bone substitutes support fast implant bed vascularization

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