The role of vascular endothelial growth factor in fractional laser resurfacing with the carbon dioxide laser

Abstract

The aim of this study was to analyze the role of vascular endothelial growth factor (VEGF) in mechanisms of cutaneous remodeling induced by fractional CO(2) laser treatment. The dorsal skin of Kunming mice was exposed to a single-pass fractional CO(2) laser treatment. Biopsies were taken 1 h, and 1, 3, 7, 14, 28 and 56 days after treatment. Skin samples VEGF expression was evaluated by immunohistochemistry and ELISA, fibroblasts by hematoxylin-eosin staining, and types I and III collagen by ELISA. Staining for VEGF was found in many types of cell including fibroblasts. The amount of VEGF in the skin of laser-treated areas had increased significantly compared to that in the control areas on days 1 and 3 (P < 0.05, P < 0.01, respectively), then decreased by day 7 after treatment and returned to the baseline level. The number of fibroblasts in the skin of the laser-treated areas had increased significantly compared to that in control areas on days 3, 7, 14, 28 and 56 after irradiation (P < 0.05, P < 0.01, P < 0.01, P < 0.01, P < 0.01, respectively). The amount of type I collagen was significantly higher in the skin of the laser-treated areas compared to that in control areas from day 28 to day 56 (P < 0.05, respectively), and type III collagen was significantly higher from day 3 to day 56 (P < 0.05, P < 0.05, P < 0.05, P < 0.05, P < 0.01, respectively). There was a positive correlation between the level of VEGF and fibroblast proliferation early stage after laser treatment (r = 0.853, P < 0.01), but there was no correlation after the first week (r = -0.124, P > 0.05). The amounts of type I and III collagen showed no significant correlations with the expression of VEGF in the late stages after laser treatment (r = 0.417, P > 0.05 and r = 0.340, P > 0.05, respectively). The results suggest that VEGF might be mainly involved in the early stages of wound healing, including the stages of acute inflammation, fibroblast proliferation and vessel formation induced by fractional CO(2) laser resurfacing.

CO₂ Laser Irradiation Restores Collagen and VEGF Expressions of HPdLF on LPS Contaminated Titanium Surface

“…7and 8). These observations conform to a recent report suggesting the eff ect of irradiation promoting VEGF expression in the early stage of the wound healing 16,17) .…”

“…At the event of anagenesis, VEGF is necessary for gingiva and bone regeneration after peri-implantitis with vascular inducer 16) . Also, CO 2 laser promotes activation of the neurogenesis of pulp cell and production of vascular endothelial growth factor (VEGF) 17,18) . As above it is necessary to carry out in a cellular level how the priodontal fibroblasts which underwent laser radiation on a titanium surface perform anagenesis as expected.…”

Vascular patterns in mature hypertrophic burn scars treated with fractional CO2 laser

“…This effect may have greater clinical efficacy for scars which have maintained erythema for longer than one year and have exhibited regression of the previously described large vessels. Interestingly, the activating effect of fractional CO 2 laser was shown in an animal study to result in an initial increase in skin VEGF expression 1 and 3 days after treatment, with a decrease in levels by day 7 after treatment . This may correspond with the well‐known side effect of temporary post‐treatment erythema.…”

Angiofibroma of Inferior Turbinate as an Unusual Complication of CO2 Laser Turbinoplasty

“…It is postulated that CO 2 laser could induce the increase of secretion of vascular endothelial growth factor and basic fibroblast growth factor. 7, 8 Nowak et al 7 showed the basic fibroblast growth factor secretion significantly increased with application more than 6 W of energy in fibroblast. However, there is still no report about vacsular endothelial growth factor secretion according to different types of laser.…”